Bluegill hepatitis B virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Metahepadnavirus

Average proteome isoelectric point is 9.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A193AUV0|A0A193AUV0_9HEPA Core OS=Bluegill hepatitis B virus OX=2169918 PE=4 SV=1
MM1 pKa = 7.66GGAQSDD7 pKa = 4.2PVADD11 pKa = 3.64AVNILLATEE20 pKa = 4.22KK21 pKa = 11.08NKK23 pKa = 9.11TLPSAARR30 pKa = 11.84IDD32 pKa = 3.85IANTPQAKK40 pKa = 9.64RR41 pKa = 11.84LAEE44 pKa = 4.21LARR47 pKa = 11.84QHH49 pKa = 5.44QQNLQPPIQAPPPPKK64 pKa = 9.53PPPIAIPPLATDD76 pKa = 4.38AAPGPVLTRR85 pKa = 11.84RR86 pKa = 11.84TNDD89 pKa = 2.96RR90 pKa = 11.84KK91 pKa = 9.41KK92 pKa = 8.34TPVYY96 pKa = 10.33HH97 pKa = 5.58VTTAPTAAATAATPTSPPQMTGFFSGWEE125 pKa = 3.77LGFPLVLQVVFFLWTKK141 pKa = 7.88IHH143 pKa = 6.51EE144 pKa = 4.95IIGKK148 pKa = 9.48LDD150 pKa = 3.32SWWIFLSSPEE160 pKa = 4.42CLTGSGGLGLHH171 pKa = 6.07PQISPHH177 pKa = 6.7LPTNCQPGCPGYY189 pKa = 9.83PWTCLRR195 pKa = 11.84RR196 pKa = 11.84FIIFLCILALFVILSLVYY214 pKa = 10.52LDD216 pKa = 4.01WIGMLFCCCCNGSDD230 pKa = 4.79CTNCTCSGSNGKK242 pKa = 8.91CCCVCKK248 pKa = 10.77DD249 pKa = 4.57CANCDD254 pKa = 3.28QCSWAFVKK262 pKa = 10.62HH263 pKa = 5.51LWALASAHH271 pKa = 5.97FSWLSSLEE279 pKa = 4.11SLSSISAHH287 pKa = 4.96SFKK290 pKa = 11.05VSVVLLIWMMWWWGITLTSIWQVSFMLFTTYY321 pKa = 10.95YY322 pKa = 9.54FLWGSTT328 pKa = 3.39

Molecular weight: 36.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A193AU86|A0A193AU86_9HEPA Core OS=Bluegill hepatitis B virus OX=2169918 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.47CIFSFLAQCQFGMVPKK18 pKa = 10.43NFYY21 pKa = 10.86HH22 pKa = 6.62FGFWHH27 pKa = 6.21GAKK30 pKa = 10.17PLGTTPTAFLNRR42 pKa = 11.84YY43 pKa = 8.63ISHH46 pKa = 7.33ALLAACQHH54 pKa = 5.33ARR56 pKa = 11.84YY57 pKa = 8.69LARR60 pKa = 11.84CQLYY64 pKa = 9.93KK65 pKa = 10.55SVSRR69 pKa = 11.84EE70 pKa = 3.48ISSFWHH76 pKa = 6.07SANSSGLGRR85 pKa = 11.84GPIFRR90 pKa = 11.84SLRR93 pKa = 11.84SRR95 pKa = 11.84EE96 pKa = 3.86PKK98 pKa = 10.42GRR100 pKa = 11.84GIEE103 pKa = 3.78ARR105 pKa = 11.84ARR107 pKa = 11.84VV108 pKa = 3.49

Molecular weight: 12.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1674

105

769

279.0

31.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.049 ± 0.55

2.33 ± 1.067

3.763 ± 0.517

2.389 ± 0.16

5.137 ± 0.629

6.272 ± 0.248

4.421 ± 0.886

5.615 ± 0.309

4.301 ± 0.22

10.932 ± 0.807

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.912 ± 0.135

3.166 ± 0.142

6.93 ± 0.667

4.421 ± 0.329

6.332 ± 1.161

8.363 ± 0.168

6.153 ± 0.527

5.376 ± 0.959

2.509 ± 0.632

2.628 ± 0.394
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski