Rhodopirellula sp. SWK7

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rhodopirellula; unclassified Rhodopirellula

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7238 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M5TDH5|M5TDH5_9BACT DinB family protein OS=Rhodopirellula sp. SWK7 OX=595460 GN=RRSWK_05618 PE=3 SV=1
MM1 pKa = 6.54TTTVLTLDD9 pKa = 3.5SKK11 pKa = 10.88RR12 pKa = 11.84WYY14 pKa = 10.06DD15 pKa = 3.87PNSSSYY21 pKa = 9.11KK22 pKa = 7.15TTGAMKK28 pKa = 10.27VGYY31 pKa = 9.41DD32 pKa = 3.52SEE34 pKa = 4.63GSYY37 pKa = 10.23EE38 pKa = 4.2QIRR41 pKa = 11.84CSYY44 pKa = 10.77RR45 pKa = 11.84FILPIGYY52 pKa = 9.88ADD54 pKa = 4.53LFITSAVLALTVDD67 pKa = 3.79ADD69 pKa = 4.12TPNYY73 pKa = 10.67GYY75 pKa = 8.91GTDD78 pKa = 4.07TIIGPHH84 pKa = 7.04DD85 pKa = 3.58ATTAFGSIDD94 pKa = 3.84LTPYY98 pKa = 9.72ATVNLLNPKK107 pKa = 10.08GIEE110 pKa = 4.04DD111 pKa = 3.74NYY113 pKa = 10.75LGYY116 pKa = 10.93GRR118 pKa = 11.84DD119 pKa = 3.65YY120 pKa = 11.0TVTQGTISVVNVDD133 pKa = 3.56LTTAVNAAIAAGKK146 pKa = 9.36IDD148 pKa = 3.6NGGINIIWDD157 pKa = 3.75QHH159 pKa = 5.7PDD161 pKa = 2.59WYY163 pKa = 10.84GYY165 pKa = 11.0DD166 pKa = 3.51LFGDD170 pKa = 4.0NNASPTLTLTHH181 pKa = 6.24VVPPDD186 pKa = 3.58PSPYY190 pKa = 10.53GLGDD194 pKa = 3.33EE195 pKa = 4.44TVLFRR200 pKa = 11.84GATLATDD207 pKa = 4.56LGPHH211 pKa = 6.37EE212 pKa = 4.8NDD214 pKa = 3.19GTLVGGLTTVADD226 pKa = 4.19AGEE229 pKa = 3.99QGIDD233 pKa = 3.41AFDD236 pKa = 3.58MDD238 pKa = 4.51GVDD241 pKa = 3.96NKK243 pKa = 10.7LVANTFSTDD252 pKa = 3.79SEE254 pKa = 4.34WTVAAWVKK262 pKa = 10.54INSGNQKK269 pKa = 10.26GCWIKK274 pKa = 11.14SNSSASDD281 pKa = 3.35EE282 pKa = 4.4YY283 pKa = 11.68GFGLGIGNTNFDD295 pKa = 3.67TNGRR299 pKa = 11.84KK300 pKa = 9.79LIVLRR305 pKa = 11.84SGVSWHH311 pKa = 5.56VSSYY315 pKa = 10.56TFASDD320 pKa = 3.12GWHH323 pKa = 6.93HH324 pKa = 6.7IAMTRR329 pKa = 11.84SGTTNKK335 pKa = 9.82VYY337 pKa = 11.02VDD339 pKa = 3.87GVLKK343 pKa = 9.36YY344 pKa = 8.79TVAFGSNSDD353 pKa = 3.15GDD355 pKa = 4.14KK356 pKa = 10.77ISFGGFDD363 pKa = 3.64VLSSYY368 pKa = 10.96YY369 pKa = 9.84RR370 pKa = 11.84YY371 pKa = 10.17CSNRR375 pKa = 11.84MDD377 pKa = 4.34DD378 pKa = 3.8CCQFSRR384 pKa = 11.84VLSQYY389 pKa = 10.3EE390 pKa = 4.06IEE392 pKa = 4.38TLASRR397 pKa = 11.84RR398 pKa = 11.84NYY400 pKa = 10.96DD401 pKa = 3.37NTWGLNGLAHH411 pKa = 7.48RR412 pKa = 11.84YY413 pKa = 8.35CATLTDD419 pKa = 5.88DD420 pKa = 3.36VDD422 pKa = 3.86DD423 pKa = 4.3WFGTAAGAFQGSMGTEE439 pKa = 3.47ADD441 pKa = 3.68AGHH444 pKa = 7.09GGTKK448 pKa = 9.97AYY450 pKa = 10.6AFNDD454 pKa = 3.5SNDD457 pKa = 3.53YY458 pKa = 11.22VSFAEE463 pKa = 4.92HH464 pKa = 6.35YY465 pKa = 10.92SPGTSDD471 pKa = 3.94FAFSCWFRR479 pKa = 11.84LDD481 pKa = 4.5ASGTTNKK488 pKa = 9.57WIYY491 pKa = 11.06SNYY494 pKa = 9.9NGNSSIAIFTNSGKK508 pKa = 9.85IRR510 pKa = 11.84VLIRR514 pKa = 11.84DD515 pKa = 3.47EE516 pKa = 4.88SNDD519 pKa = 3.15EE520 pKa = 3.93VRR522 pKa = 11.84FDD524 pKa = 3.79STTTPAANTWYY535 pKa = 9.98HH536 pKa = 6.97LAVTWDD542 pKa = 3.65ASDD545 pKa = 3.23HH546 pKa = 4.73QARR549 pKa = 11.84LFINGTYY556 pKa = 7.39EE557 pKa = 3.77TSAINASIGNVNLTGGGVPCLGAYY581 pKa = 9.55SAGTWDD587 pKa = 3.04SNYY590 pKa = 10.02RR591 pKa = 11.84FAGGIEE597 pKa = 3.97DD598 pKa = 3.68VMIFRR603 pKa = 11.84SEE605 pKa = 3.82ISDD608 pKa = 3.43ATIASIASNPPGWDD622 pKa = 3.2GVEE625 pKa = 4.13VSAVVATIMLDD636 pKa = 3.63DD637 pKa = 3.89VTMSAAAVSVVSGEE651 pKa = 4.17SGIVLDD657 pKa = 4.82DD658 pKa = 3.58VSMAAWGVSCVSSEE672 pKa = 4.1STIVLDD678 pKa = 4.68DD679 pKa = 3.58VAMDD683 pKa = 3.74SLGTQLSVARR693 pKa = 11.84ASATITLDD701 pKa = 3.57DD702 pKa = 3.51VTMIASADD710 pKa = 4.05SIVDD714 pKa = 3.58ASSLIILDD722 pKa = 4.16DD723 pKa = 3.58VTMVAVAGAVGNASSTITLDD743 pKa = 3.61DD744 pKa = 3.42VSMITISEE752 pKa = 4.66LIVVGGASITLDD764 pKa = 3.76DD765 pKa = 4.37VSMSAAGEE773 pKa = 4.3STVTGVSTIVLDD785 pKa = 4.02NVTLSSGSQTSVSATSTIVLDD806 pKa = 4.31DD807 pKa = 4.08VEE809 pKa = 5.2LSIDD813 pKa = 3.56VPSNNRR819 pKa = 11.84ISVVLDD825 pKa = 3.62DD826 pKa = 3.96VTMASIGANIVGADD840 pKa = 3.51ATIVLDD846 pKa = 4.91DD847 pKa = 3.72ISVTVVAAVSSLTGDD862 pKa = 3.72DD863 pKa = 3.66VLFPNDD869 pKa = 4.96LFPGDD874 pKa = 4.29LFTTEE879 pKa = 4.04VFVV882 pKa = 4.36

Molecular weight: 93.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M5TUN0|M5TUN0_9BACT Uncharacterized protein OS=Rhodopirellula sp. SWK7 OX=595460 GN=RRSWK_00083 PE=4 SV=1
MM1 pKa = 6.58VRR3 pKa = 11.84LWIARR8 pKa = 11.84VRR10 pKa = 11.84IGSLVGGGFLTRR22 pKa = 11.84SASVSVILSALGVRR36 pKa = 11.84LIRR39 pKa = 11.84VILTAA44 pKa = 4.64

Molecular weight: 4.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7238

0

7238

2568549

34

9407

354.9

39.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.431 ± 0.031

1.135 ± 0.015

6.543 ± 0.037

6.162 ± 0.031

3.698 ± 0.021

7.721 ± 0.042

2.223 ± 0.019

5.18 ± 0.02

3.38 ± 0.035

9.131 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.347 ± 0.019

3.46 ± 0.027

5.148 ± 0.026

3.736 ± 0.018

6.683 ± 0.04

7.055 ± 0.03

5.907 ± 0.043

7.217 ± 0.023

1.485 ± 0.015

2.358 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski