Amino acid dipepetide frequency for Nostoc phage N1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.646AlaAla: 3.646 ± 0.763
0.599AlaCys: 0.599 ± 0.188
3.102AlaAsp: 3.102 ± 0.354
3.102AlaGlu: 3.102 ± 0.378
2.667AlaPhe: 2.667 ± 0.462
3.592AlaGly: 3.592 ± 0.456
0.544AlaHis: 0.544 ± 0.214
6.912AlaIle: 6.912 ± 0.571
3.156AlaLys: 3.156 ± 0.445
5.17AlaLeu: 5.17 ± 0.61
1.361AlaMet: 1.361 ± 0.328
3.864AlaAsn: 3.864 ± 0.57
1.687AlaPro: 1.687 ± 0.217
2.014AlaGln: 2.014 ± 0.383
1.959AlaArg: 1.959 ± 0.292
4.136AlaSer: 4.136 ± 0.696
3.483AlaThr: 3.483 ± 0.514
3.048AlaVal: 3.048 ± 0.416
0.49AlaTrp: 0.49 ± 0.166
2.231AlaTyr: 2.231 ± 0.322
0.0AlaXaa: 0.0 ± 0.0
Cys
0.381CysAla: 0.381 ± 0.127
0.272CysCys: 0.272 ± 0.094
0.762CysAsp: 0.762 ± 0.199
0.871CysGlu: 0.871 ± 0.271
0.653CysPhe: 0.653 ± 0.171
0.49CysGly: 0.49 ± 0.172
0.218CysHis: 0.218 ± 0.108
0.925CysIle: 0.925 ± 0.256
0.653CysLys: 0.653 ± 0.174
0.871CysLeu: 0.871 ± 0.209
0.109CysMet: 0.109 ± 0.07
0.381CysAsn: 0.381 ± 0.13
0.327CysPro: 0.327 ± 0.118
0.599CysGln: 0.599 ± 0.177
0.599CysArg: 0.599 ± 0.177
0.49CysSer: 0.49 ± 0.158
0.435CysThr: 0.435 ± 0.136
0.435CysVal: 0.435 ± 0.171
0.272CysTrp: 0.272 ± 0.103
0.599CysTyr: 0.599 ± 0.171
0.0CysXaa: 0.0 ± 0.0
Asp
2.776AspAla: 2.776 ± 0.439
0.49AspCys: 0.49 ± 0.131
3.429AspAsp: 3.429 ± 0.385
3.483AspGlu: 3.483 ± 0.517
3.483AspPhe: 3.483 ± 0.473
3.81AspGly: 3.81 ± 0.525
0.925AspHis: 0.925 ± 0.207
4.136AspIle: 4.136 ± 0.487
3.646AspLys: 3.646 ± 0.436
5.878AspLeu: 5.878 ± 0.596
1.034AspMet: 1.034 ± 0.21
3.429AspAsn: 3.429 ± 0.477
2.776AspPro: 2.776 ± 0.4
1.905AspGln: 1.905 ± 0.303
2.014AspArg: 2.014 ± 0.395
5.17AspSer: 5.17 ± 0.527
3.265AspThr: 3.265 ± 0.432
4.027AspVal: 4.027 ± 0.468
1.143AspTrp: 1.143 ± 0.277
2.558AspTyr: 2.558 ± 0.334
0.0AspXaa: 0.0 ± 0.0
Glu
3.211GluAla: 3.211 ± 0.426
0.653GluCys: 0.653 ± 0.213
2.503GluAsp: 2.503 ± 0.438
2.939GluGlu: 2.939 ± 0.464
3.211GluPhe: 3.211 ± 0.383
2.884GluGly: 2.884 ± 0.436
1.088GluHis: 1.088 ± 0.279
4.952GluIle: 4.952 ± 0.577
4.408GluLys: 4.408 ± 0.628
5.442GluLeu: 5.442 ± 0.494
1.252GluMet: 1.252 ± 0.246
4.898GluAsn: 4.898 ± 0.474
1.578GluPro: 1.578 ± 0.263
2.449GluGln: 2.449 ± 0.398
2.503GluArg: 2.503 ± 0.333
4.19GluSer: 4.19 ± 0.672
3.918GluThr: 3.918 ± 0.43
2.83GluVal: 2.83 ± 0.461
0.816GluTrp: 0.816 ± 0.236
2.884GluTyr: 2.884 ± 0.443
0.0GluXaa: 0.0 ± 0.0
Phe
2.122PheAla: 2.122 ± 0.445
0.599PheCys: 0.599 ± 0.166
3.32PheAsp: 3.32 ± 0.423
2.612PheGlu: 2.612 ± 0.373
1.905PhePhe: 1.905 ± 0.378
2.884PheGly: 2.884 ± 0.389
0.599PheHis: 0.599 ± 0.153
4.027PheIle: 4.027 ± 0.438
3.973PheLys: 3.973 ± 0.526
4.136PheLeu: 4.136 ± 0.488
0.98PheMet: 0.98 ± 0.226
3.537PheAsn: 3.537 ± 0.422
2.014PhePro: 2.014 ± 0.312
1.687PheGln: 1.687 ± 0.277
1.687PheArg: 1.687 ± 0.298
3.32PheSer: 3.32 ± 0.379
4.027PheThr: 4.027 ± 0.417
2.612PheVal: 2.612 ± 0.329
0.707PheTrp: 0.707 ± 0.189
1.741PheTyr: 1.741 ± 0.289
0.0PheXaa: 0.0 ± 0.0
Gly
3.537GlyAla: 3.537 ± 0.545
0.599GlyCys: 0.599 ± 0.205
3.374GlyAsp: 3.374 ± 0.404
2.721GlyGlu: 2.721 ± 0.394
3.81GlyPhe: 3.81 ± 0.587
3.918GlyGly: 3.918 ± 0.574
0.816GlyHis: 0.816 ± 0.297
4.517GlyIle: 4.517 ± 0.431
5.061GlyLys: 5.061 ± 0.509
4.898GlyLeu: 4.898 ± 0.538
0.925GlyMet: 0.925 ± 0.195
4.299GlyAsn: 4.299 ± 0.57
0.0GlyPro: 0.0 ± 0.0
2.068GlyGln: 2.068 ± 0.32
2.068GlyArg: 2.068 ± 0.318
4.027GlySer: 4.027 ± 0.507
4.571GlyThr: 4.571 ± 0.645
5.116GlyVal: 5.116 ± 0.587
1.143GlyTrp: 1.143 ± 0.268
3.32GlyTyr: 3.32 ± 0.475
0.0GlyXaa: 0.0 ± 0.0
His
0.653HisAla: 0.653 ± 0.177
0.381HisCys: 0.381 ± 0.145
0.762HisAsp: 0.762 ± 0.188
0.49HisGlu: 0.49 ± 0.176
0.218HisPhe: 0.218 ± 0.109
1.415HisGly: 1.415 ± 0.357
0.381HisHis: 0.381 ± 0.159
1.415HisIle: 1.415 ± 0.27
0.816HisLys: 0.816 ± 0.223
1.905HisLeu: 1.905 ± 0.303
0.109HisMet: 0.109 ± 0.075
0.816HisAsn: 0.816 ± 0.186
1.197HisPro: 1.197 ± 0.242
0.599HisGln: 0.599 ± 0.215
0.871HisArg: 0.871 ± 0.234
0.98HisSer: 0.98 ± 0.219
0.762HisThr: 0.762 ± 0.258
0.925HisVal: 0.925 ± 0.196
0.544HisTrp: 0.544 ± 0.184
0.707HisTyr: 0.707 ± 0.157
0.0HisXaa: 0.0 ± 0.0
Ile
6.912IleAla: 6.912 ± 0.614
0.653IleCys: 0.653 ± 0.184
6.912IleAsp: 6.912 ± 0.627
6.095IleGlu: 6.095 ± 0.602
2.993IlePhe: 2.993 ± 0.405
3.918IleGly: 3.918 ± 0.53
1.361IleHis: 1.361 ± 0.36
5.714IleIle: 5.714 ± 0.523
6.15IleLys: 6.15 ± 0.624
6.204IleLeu: 6.204 ± 0.57
1.143IleMet: 1.143 ± 0.26
5.497IleAsn: 5.497 ± 0.599
2.993IlePro: 2.993 ± 0.421
2.83IleGln: 2.83 ± 0.358
3.048IleArg: 3.048 ± 0.45
6.095IleSer: 6.095 ± 0.552
4.354IleThr: 4.354 ± 0.548
5.388IleVal: 5.388 ± 0.575
0.544IleTrp: 0.544 ± 0.171
3.048IleTyr: 3.048 ± 0.419
0.0IleXaa: 0.0 ± 0.0
Lys
4.408LysAla: 4.408 ± 0.461
0.327LysCys: 0.327 ± 0.132
3.973LysAsp: 3.973 ± 0.55
4.408LysGlu: 4.408 ± 0.519
2.395LysPhe: 2.395 ± 0.357
3.102LysGly: 3.102 ± 0.374
1.959LysHis: 1.959 ± 0.373
5.932LysIle: 5.932 ± 0.541
5.17LysLys: 5.17 ± 0.695
6.15LysLeu: 6.15 ± 0.7
1.361LysMet: 1.361 ± 0.298
4.626LysAsn: 4.626 ± 0.541
3.864LysPro: 3.864 ± 0.509
2.721LysGln: 2.721 ± 0.426
3.156LysArg: 3.156 ± 0.564
4.354LysSer: 4.354 ± 0.529
4.408LysThr: 4.408 ± 0.539
3.864LysVal: 3.864 ± 0.515
0.435LysTrp: 0.435 ± 0.148
3.211LysTyr: 3.211 ± 0.439
0.0LysXaa: 0.0 ± 0.0
Leu
4.245LeuAla: 4.245 ± 0.553
0.816LeuCys: 0.816 ± 0.197
5.497LeuAsp: 5.497 ± 0.511
6.422LeuGlu: 6.422 ± 0.659
4.463LeuPhe: 4.463 ± 0.579
4.789LeuGly: 4.789 ± 0.445
1.034LeuHis: 1.034 ± 0.212
5.878LeuIle: 5.878 ± 0.633
6.639LeuLys: 6.639 ± 0.78
7.238LeuLeu: 7.238 ± 0.644
2.177LeuMet: 2.177 ± 0.371
5.279LeuAsn: 5.279 ± 0.447
3.918LeuPro: 3.918 ± 0.557
3.592LeuGln: 3.592 ± 0.479
3.374LeuArg: 3.374 ± 0.471
6.912LeuSer: 6.912 ± 0.655
5.714LeuThr: 5.714 ± 0.59
4.082LeuVal: 4.082 ± 0.451
0.599LeuTrp: 0.599 ± 0.142
3.646LeuTyr: 3.646 ± 0.418
0.0LeuXaa: 0.0 ± 0.0
Met
0.816MetAla: 0.816 ± 0.286
0.163MetCys: 0.163 ± 0.1
0.707MetAsp: 0.707 ± 0.19
0.871MetGlu: 0.871 ± 0.251
0.762MetPhe: 0.762 ± 0.156
0.871MetGly: 0.871 ± 0.248
0.109MetHis: 0.109 ± 0.068
2.014MetIle: 2.014 ± 0.296
1.85MetLys: 1.85 ± 0.35
1.796MetLeu: 1.796 ± 0.386
0.925MetMet: 0.925 ± 0.227
1.959MetAsn: 1.959 ± 0.355
0.599MetPro: 0.599 ± 0.182
0.762MetGln: 0.762 ± 0.199
0.925MetArg: 0.925 ± 0.217
2.449MetSer: 2.449 ± 0.371
1.088MetThr: 1.088 ± 0.238
1.306MetVal: 1.306 ± 0.247
0.163MetTrp: 0.163 ± 0.088
0.599MetTyr: 0.599 ± 0.144
0.0MetXaa: 0.0 ± 0.0
Asn
3.918AsnAla: 3.918 ± 0.493
0.599AsnCys: 0.599 ± 0.209
4.082AsnAsp: 4.082 ± 0.414
3.374AsnGlu: 3.374 ± 0.535
2.558AsnPhe: 2.558 ± 0.307
4.299AsnGly: 4.299 ± 0.523
0.816AsnHis: 0.816 ± 0.22
5.769AsnIle: 5.769 ± 0.678
4.027AsnLys: 4.027 ± 0.498
4.844AsnLeu: 4.844 ± 0.529
1.85AsnMet: 1.85 ± 0.307
3.864AsnAsn: 3.864 ± 0.51
2.231AsnPro: 2.231 ± 0.358
2.449AsnGln: 2.449 ± 0.39
2.83AsnArg: 2.83 ± 0.422
4.517AsnSer: 4.517 ± 0.58
3.429AsnThr: 3.429 ± 0.374
4.082AsnVal: 4.082 ± 0.542
0.544AsnTrp: 0.544 ± 0.145
2.286AsnTyr: 2.286 ± 0.332
0.0AsnXaa: 0.0 ± 0.0
Pro
1.796ProAla: 1.796 ± 0.236
0.435ProCys: 0.435 ± 0.17
2.558ProAsp: 2.558 ± 0.404
3.918ProGlu: 3.918 ± 0.576
1.796ProPhe: 1.796 ± 0.308
2.884ProGly: 2.884 ± 0.4
0.599ProHis: 0.599 ± 0.221
2.776ProIle: 2.776 ± 0.348
2.612ProLys: 2.612 ± 0.331
2.558ProLeu: 2.558 ± 0.424
0.599ProMet: 0.599 ± 0.206
1.959ProAsn: 1.959 ± 0.271
1.578ProPro: 1.578 ± 0.316
1.741ProGln: 1.741 ± 0.307
1.578ProArg: 1.578 ± 0.273
2.503ProSer: 2.503 ± 0.325
2.231ProThr: 2.231 ± 0.384
2.667ProVal: 2.667 ± 0.282
0.218ProTrp: 0.218 ± 0.128
1.197ProTyr: 1.197 ± 0.216
0.0ProXaa: 0.0 ± 0.0
Gln
2.503GlnAla: 2.503 ± 0.418
0.218GlnCys: 0.218 ± 0.104
1.578GlnAsp: 1.578 ± 0.312
1.633GlnGlu: 1.633 ± 0.343
1.524GlnPhe: 1.524 ± 0.356
2.612GlnGly: 2.612 ± 0.385
0.925GlnHis: 0.925 ± 0.204
3.048GlnIle: 3.048 ± 0.378
2.068GlnLys: 2.068 ± 0.331
3.701GlnLeu: 3.701 ± 0.462
1.034GlnMet: 1.034 ± 0.236
2.503GlnAsn: 2.503 ± 0.367
1.633GlnPro: 1.633 ± 0.239
2.231GlnGln: 2.231 ± 0.425
2.014GlnArg: 2.014 ± 0.386
3.265GlnSer: 3.265 ± 0.461
2.395GlnThr: 2.395 ± 0.39
2.503GlnVal: 2.503 ± 0.348
0.707GlnTrp: 0.707 ± 0.231
1.633GlnTyr: 1.633 ± 0.327
0.0GlnXaa: 0.0 ± 0.0
Arg
1.687ArgAla: 1.687 ± 0.305
0.653ArgCys: 0.653 ± 0.196
1.796ArgAsp: 1.796 ± 0.308
2.395ArgGlu: 2.395 ± 0.335
2.721ArgPhe: 2.721 ± 0.418
2.286ArgGly: 2.286 ± 0.387
0.707ArgHis: 0.707 ± 0.181
3.592ArgIle: 3.592 ± 0.438
3.048ArgLys: 3.048 ± 0.585
5.007ArgLeu: 5.007 ± 0.575
0.871ArgMet: 0.871 ± 0.207
1.796ArgAsn: 1.796 ± 0.319
0.871ArgPro: 0.871 ± 0.232
1.741ArgGln: 1.741 ± 0.317
0.98ArgArg: 0.98 ± 0.28
2.231ArgSer: 2.231 ± 0.272
2.34ArgThr: 2.34 ± 0.405
2.558ArgVal: 2.558 ± 0.38
0.435ArgTrp: 0.435 ± 0.174
1.741ArgTyr: 1.741 ± 0.294
0.0ArgXaa: 0.0 ± 0.0
Ser
3.918SerAla: 3.918 ± 0.529
0.599SerCys: 0.599 ± 0.183
5.17SerAsp: 5.17 ± 0.524
3.537SerGlu: 3.537 ± 0.491
4.354SerPhe: 4.354 ± 0.403
6.041SerGly: 6.041 ± 0.547
0.98SerHis: 0.98 ± 0.206
5.17SerIle: 5.17 ± 0.498
4.517SerLys: 4.517 ± 0.527
5.279SerLeu: 5.279 ± 0.707
1.633SerMet: 1.633 ± 0.297
3.646SerAsn: 3.646 ± 0.469
3.32SerPro: 3.32 ± 0.384
2.177SerGln: 2.177 ± 0.32
2.884SerArg: 2.884 ± 0.306
5.007SerSer: 5.007 ± 0.482
3.918SerThr: 3.918 ± 0.527
6.15SerVal: 6.15 ± 0.677
0.707SerTrp: 0.707 ± 0.219
3.211SerTyr: 3.211 ± 0.368
0.0SerXaa: 0.0 ± 0.0
Thr
4.354ThrAla: 4.354 ± 0.674
0.599ThrCys: 0.599 ± 0.156
3.374ThrAsp: 3.374 ± 0.442
3.156ThrGlu: 3.156 ± 0.43
2.34ThrPhe: 2.34 ± 0.3
4.245ThrGly: 4.245 ± 0.665
1.469ThrHis: 1.469 ± 0.395
6.15ThrIle: 6.15 ± 0.519
3.102ThrLys: 3.102 ± 0.45
5.061ThrLeu: 5.061 ± 0.608
0.871ThrMet: 0.871 ± 0.189
3.32ThrAsn: 3.32 ± 0.445
3.973ThrPro: 3.973 ± 0.473
2.177ThrGln: 2.177 ± 0.397
2.286ThrArg: 2.286 ± 0.332
4.082ThrSer: 4.082 ± 0.564
5.116ThrThr: 5.116 ± 0.81
4.245ThrVal: 4.245 ± 0.501
0.707ThrTrp: 0.707 ± 0.192
2.503ThrTyr: 2.503 ± 0.334
0.0ThrXaa: 0.0 ± 0.0
Val
3.265ValAla: 3.265 ± 0.538
0.925ValCys: 0.925 ± 0.262
3.537ValAsp: 3.537 ± 0.473
3.537ValGlu: 3.537 ± 0.465
4.027ValPhe: 4.027 ± 0.498
4.245ValGly: 4.245 ± 0.494
0.49ValHis: 0.49 ± 0.18
4.517ValIle: 4.517 ± 0.487
4.463ValLys: 4.463 ± 0.523
5.279ValLeu: 5.279 ± 0.481
1.252ValMet: 1.252 ± 0.242
3.81ValAsn: 3.81 ± 0.526
2.177ValPro: 2.177 ± 0.386
2.231ValGln: 2.231 ± 0.306
2.503ValArg: 2.503 ± 0.422
5.497ValSer: 5.497 ± 0.548
4.408ValThr: 4.408 ± 0.615
4.027ValVal: 4.027 ± 0.381
0.435ValTrp: 0.435 ± 0.135
2.122ValTyr: 2.122 ± 0.396
0.0ValXaa: 0.0 ± 0.0
Trp
0.544TrpAla: 0.544 ± 0.193
0.218TrpCys: 0.218 ± 0.101
0.925TrpAsp: 0.925 ± 0.212
0.871TrpGlu: 0.871 ± 0.222
0.707TrpPhe: 0.707 ± 0.199
0.49TrpGly: 0.49 ± 0.15
0.054TrpHis: 0.054 ± 0.048
0.871TrpIle: 0.871 ± 0.253
0.871TrpLys: 0.871 ± 0.219
1.034TrpLeu: 1.034 ± 0.227
0.435TrpMet: 0.435 ± 0.138
0.49TrpAsn: 0.49 ± 0.163
0.0TrpPro: 0.0 ± 0.0
0.707TrpGln: 0.707 ± 0.187
0.435TrpArg: 0.435 ± 0.141
0.762TrpSer: 0.762 ± 0.207
0.599TrpThr: 0.599 ± 0.195
0.925TrpVal: 0.925 ± 0.185
0.109TrpTrp: 0.109 ± 0.068
0.327TrpTyr: 0.327 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.068TyrAla: 2.068 ± 0.303
0.544TyrCys: 0.544 ± 0.198
2.014TyrAsp: 2.014 ± 0.263
2.177TyrGlu: 2.177 ± 0.337
1.905TyrPhe: 1.905 ± 0.304
2.014TyrGly: 2.014 ± 0.375
0.871TyrHis: 0.871 ± 0.229
3.537TyrIle: 3.537 ± 0.377
3.646TyrLys: 3.646 ± 0.447
3.864TyrLeu: 3.864 ± 0.385
0.762TyrMet: 0.762 ± 0.181
2.395TyrAsn: 2.395 ± 0.331
1.469TyrPro: 1.469 ± 0.357
3.048TyrGln: 3.048 ± 0.459
1.741TyrArg: 1.741 ± 0.263
2.231TyrSer: 2.231 ± 0.276
2.667TyrThr: 2.667 ± 0.401
1.959TyrVal: 1.959 ± 0.313
0.653TyrTrp: 0.653 ± 0.196
2.177TyrTyr: 2.177 ± 0.327
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (18376 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski