Amino acid dipepetide frequency for Streptomyces sp. CdTB01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.514AlaAla: 21.514 ± 0.15
1.113AlaCys: 1.113 ± 0.02
8.312AlaAsp: 8.312 ± 0.062
8.536AlaGlu: 8.536 ± 0.081
3.568AlaPhe: 3.568 ± 0.04
12.787AlaGly: 12.787 ± 0.083
3.112AlaHis: 3.112 ± 0.035
3.363AlaIle: 3.363 ± 0.04
2.886AlaLys: 2.886 ± 0.044
14.784AlaLeu: 14.784 ± 0.109
2.445AlaMet: 2.445 ± 0.03
1.924AlaAsn: 1.924 ± 0.03
6.954AlaPro: 6.954 ± 0.069
3.928AlaGln: 3.928 ± 0.041
10.427AlaArg: 10.427 ± 0.078
6.168AlaSer: 6.168 ± 0.052
7.056AlaThr: 7.056 ± 0.056
12.451AlaVal: 12.451 ± 0.083
1.954AlaTrp: 1.954 ± 0.029
2.852AlaTyr: 2.852 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
1.104CysAla: 1.104 ± 0.021
0.103CysCys: 0.103 ± 0.006
0.47CysAsp: 0.47 ± 0.014
0.386CysGlu: 0.386 ± 0.013
0.225CysPhe: 0.225 ± 0.009
0.936CysGly: 0.936 ± 0.02
0.206CysHis: 0.206 ± 0.008
0.167CysIle: 0.167 ± 0.009
0.124CysLys: 0.124 ± 0.008
0.793CysLeu: 0.793 ± 0.018
0.115CysMet: 0.115 ± 0.006
0.136CysAsn: 0.136 ± 0.007
0.517CysPro: 0.517 ± 0.014
0.166CysGln: 0.166 ± 0.008
0.611CysArg: 0.611 ± 0.018
0.447CysSer: 0.447 ± 0.012
0.524CysThr: 0.524 ± 0.015
0.684CysVal: 0.684 ± 0.015
0.129CysTrp: 0.129 ± 0.006
0.16CysTyr: 0.16 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.685AspAla: 7.685 ± 0.069
0.438AspCys: 0.438 ± 0.012
3.524AspAsp: 3.524 ± 0.039
3.746AspGlu: 3.746 ± 0.044
1.639AspPhe: 1.639 ± 0.027
6.309AspGly: 6.309 ± 0.06
1.441AspHis: 1.441 ± 0.025
1.839AspIle: 1.839 ± 0.027
1.19AspLys: 1.19 ± 0.023
6.321AspLeu: 6.321 ± 0.053
0.852AspMet: 0.852 ± 0.017
0.993AspAsn: 0.993 ± 0.02
4.372AspPro: 4.372 ± 0.046
1.467AspGln: 1.467 ± 0.024
4.878AspArg: 4.878 ± 0.051
2.523AspSer: 2.523 ± 0.033
3.277AspThr: 3.277 ± 0.039
4.865AspVal: 4.865 ± 0.041
1.059AspTrp: 1.059 ± 0.022
1.163AspTyr: 1.163 ± 0.021
0.0AspXaa: 0.0 ± 0.0
Glu
7.276GluAla: 7.276 ± 0.078
0.346GluCys: 0.346 ± 0.011
2.787GluAsp: 2.787 ± 0.038
3.565GluGlu: 3.565 ± 0.052
1.417GluPhe: 1.417 ± 0.025
4.171GluGly: 4.171 ± 0.042
1.542GluHis: 1.542 ± 0.025
2.202GluIle: 2.202 ± 0.031
1.361GluLys: 1.361 ± 0.024
6.585GluLeu: 6.585 ± 0.059
0.857GluMet: 0.857 ± 0.019
0.968GluAsn: 0.968 ± 0.02
3.225GluPro: 3.225 ± 0.043
2.21GluGln: 2.21 ± 0.031
5.437GluArg: 5.437 ± 0.049
2.368GluSer: 2.368 ± 0.031
2.831GluThr: 2.831 ± 0.034
4.373GluVal: 4.373 ± 0.047
0.753GluTrp: 0.753 ± 0.017
1.072GluTyr: 1.072 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.654PheAla: 3.654 ± 0.041
0.259PheCys: 0.259 ± 0.011
1.942PheAsp: 1.942 ± 0.032
1.386PheGlu: 1.386 ± 0.02
0.877PhePhe: 0.877 ± 0.019
2.992PheGly: 2.992 ± 0.04
0.645PheHis: 0.645 ± 0.016
0.694PheIle: 0.694 ± 0.016
0.537PheLys: 0.537 ± 0.014
2.657PheLeu: 2.657 ± 0.038
0.415PheMet: 0.415 ± 0.014
0.562PheAsn: 0.562 ± 0.017
1.38PhePro: 1.38 ± 0.024
0.674PheGln: 0.674 ± 0.014
1.851PheArg: 1.851 ± 0.025
1.473PheSer: 1.473 ± 0.026
2.033PheThr: 2.033 ± 0.029
2.292PheVal: 2.292 ± 0.033
0.44PheTrp: 0.44 ± 0.012
0.581PheTyr: 0.581 ± 0.015
0.0PheXaa: 0.0 ± 0.0
Gly
10.85GlyAla: 10.85 ± 0.072
0.838GlyCys: 0.838 ± 0.02
5.099GlyAsp: 5.099 ± 0.058
4.946GlyGlu: 4.946 ± 0.045
2.88GlyPhe: 2.88 ± 0.039
8.579GlyGly: 8.579 ± 0.065
2.434GlyHis: 2.434 ± 0.031
3.367GlyIle: 3.367 ± 0.038
2.442GlyLys: 2.442 ± 0.037
9.434GlyLeu: 9.434 ± 0.065
2.016GlyMet: 2.016 ± 0.032
1.748GlyAsn: 1.748 ± 0.03
4.987GlyPro: 4.987 ± 0.045
2.631GlyGln: 2.631 ± 0.035
7.819GlyArg: 7.819 ± 0.058
5.391GlySer: 5.391 ± 0.048
6.389GlyThr: 6.389 ± 0.058
7.443GlyVal: 7.443 ± 0.057
1.738GlyTrp: 1.738 ± 0.031
2.3GlyTyr: 2.3 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.869HisAla: 2.869 ± 0.033
0.229HisCys: 0.229 ± 0.009
1.43HisAsp: 1.43 ± 0.025
1.253HisGlu: 1.253 ± 0.019
0.658HisPhe: 0.658 ± 0.015
2.504HisGly: 2.504 ± 0.027
0.762HisHis: 0.762 ± 0.021
0.688HisIle: 0.688 ± 0.016
0.389HisLys: 0.389 ± 0.013
2.495HisLeu: 2.495 ± 0.032
0.36HisMet: 0.36 ± 0.014
0.399HisAsn: 0.399 ± 0.013
1.883HisPro: 1.883 ± 0.03
0.672HisGln: 0.672 ± 0.015
2.167HisArg: 2.167 ± 0.027
1.02HisSer: 1.02 ± 0.019
1.442HisThr: 1.442 ± 0.026
1.789HisVal: 1.789 ± 0.024
0.393HisTrp: 0.393 ± 0.011
0.502HisTyr: 0.502 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.722IleAla: 4.722 ± 0.049
0.271IleCys: 0.271 ± 0.009
2.174IleAsp: 2.174 ± 0.034
1.879IleGlu: 1.879 ± 0.03
0.654IlePhe: 0.654 ± 0.019
3.422IleGly: 3.422 ± 0.039
0.603IleHis: 0.603 ± 0.014
0.827IleIle: 0.827 ± 0.02
0.72IleLys: 0.72 ± 0.016
2.408IleLeu: 2.408 ± 0.035
0.44IleMet: 0.44 ± 0.014
0.69IleAsn: 0.69 ± 0.016
1.692IlePro: 1.692 ± 0.025
0.704IleGln: 0.704 ± 0.019
2.185IleArg: 2.185 ± 0.026
1.606IleSer: 1.606 ± 0.023
2.206IleThr: 2.206 ± 0.029
2.755IleVal: 2.755 ± 0.035
0.377IleTrp: 0.377 ± 0.012
0.521IleTyr: 0.521 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
3.012LysAla: 3.012 ± 0.045
0.117LysCys: 0.117 ± 0.007
1.37LysAsp: 1.37 ± 0.026
1.168LysGlu: 1.168 ± 0.022
0.46LysPhe: 0.46 ± 0.014
1.906LysGly: 1.906 ± 0.033
0.429LysHis: 0.429 ± 0.012
0.855LysIle: 0.855 ± 0.017
0.889LysLys: 0.889 ± 0.026
1.989LysLeu: 1.989 ± 0.03
0.361LysMet: 0.361 ± 0.012
0.542LysAsn: 0.542 ± 0.016
1.326LysPro: 1.326 ± 0.027
0.711LysGln: 0.711 ± 0.016
1.37LysArg: 1.37 ± 0.023
1.132LysSer: 1.132 ± 0.021
1.329LysThr: 1.329 ± 0.027
1.978LysVal: 1.978 ± 0.031
0.296LysTrp: 0.296 ± 0.012
0.481LysTyr: 0.481 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
15.274LeuAla: 15.274 ± 0.105
0.863LeuCys: 0.863 ± 0.018
6.682LeuAsp: 6.682 ± 0.053
4.62LeuGlu: 4.62 ± 0.046
2.646LeuPhe: 2.646 ± 0.036
9.319LeuGly: 9.319 ± 0.067
2.433LeuHis: 2.433 ± 0.031
3.2LeuIle: 3.2 ± 0.037
2.108LeuLys: 2.108 ± 0.031
11.601LeuLeu: 11.601 ± 0.095
1.65LeuMet: 1.65 ± 0.021
1.741LeuAsn: 1.741 ± 0.022
6.563LeuPro: 6.563 ± 0.058
2.314LeuGln: 2.314 ± 0.032
8.73LeuArg: 8.73 ± 0.072
5.302LeuSer: 5.302 ± 0.054
7.147LeuThr: 7.147 ± 0.061
8.892LeuVal: 8.892 ± 0.067
1.366LeuTrp: 1.366 ± 0.024
1.941LeuTyr: 1.941 ± 0.026
0.0LeuXaa: 0.0 ± 0.0
Met
2.197MetAla: 2.197 ± 0.033
0.144MetCys: 0.144 ± 0.008
0.881MetAsp: 0.881 ± 0.018
0.734MetGlu: 0.734 ± 0.016
0.465MetPhe: 0.465 ± 0.014
1.318MetGly: 1.318 ± 0.022
0.373MetHis: 0.373 ± 0.013
0.664MetIle: 0.664 ± 0.018
0.415MetLys: 0.415 ± 0.012
1.672MetLeu: 1.672 ± 0.022
0.306MetMet: 0.306 ± 0.012
0.459MetAsn: 0.459 ± 0.013
1.126MetPro: 1.126 ± 0.02
0.446MetGln: 0.446 ± 0.014
1.425MetArg: 1.425 ± 0.023
1.319MetSer: 1.319 ± 0.02
1.586MetThr: 1.586 ± 0.023
1.259MetVal: 1.259 ± 0.022
0.231MetTrp: 0.231 ± 0.009
0.322MetTyr: 0.322 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
2.243AsnAla: 2.243 ± 0.028
0.165AsnCys: 0.165 ± 0.008
0.974AsnAsp: 0.974 ± 0.021
0.803AsnGlu: 0.803 ± 0.016
0.498AsnPhe: 0.498 ± 0.014
1.939AsnGly: 1.939 ± 0.033
0.401AsnHis: 0.401 ± 0.012
0.65AsnIle: 0.65 ± 0.017
0.427AsnLys: 0.427 ± 0.014
1.639AsnLeu: 1.639 ± 0.031
0.305AsnMet: 0.305 ± 0.011
0.429AsnAsn: 0.429 ± 0.014
1.312AsnPro: 1.312 ± 0.024
0.531AsnGln: 0.531 ± 0.014
1.244AsnArg: 1.244 ± 0.021
0.979AsnSer: 0.979 ± 0.022
1.123AsnThr: 1.123 ± 0.022
1.389AsnVal: 1.389 ± 0.025
0.318AsnTrp: 0.318 ± 0.01
0.395AsnTyr: 0.395 ± 0.012
0.0AsnXaa: 0.0 ± 0.0
Pro
8.578ProAla: 8.578 ± 0.064
0.348ProCys: 0.348 ± 0.011
4.378ProAsp: 4.378 ± 0.044
4.151ProGlu: 4.151 ± 0.045
1.528ProPhe: 1.528 ± 0.025
6.37ProGly: 6.37 ± 0.055
1.486ProHis: 1.486 ± 0.025
1.23ProIle: 1.23 ± 0.024
1.172ProLys: 1.172 ± 0.025
5.442ProLeu: 5.442 ± 0.049
0.981ProMet: 0.981 ± 0.019
0.906ProAsn: 0.906 ± 0.019
3.446ProPro: 3.446 ± 0.056
1.79ProGln: 1.79 ± 0.034
3.986ProArg: 3.986 ± 0.039
3.213ProSer: 3.213 ± 0.04
3.2ProThr: 3.2 ± 0.035
5.464ProVal: 5.464 ± 0.049
0.89ProTrp: 0.89 ± 0.018
1.464ProTyr: 1.464 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.821GlnAla: 3.821 ± 0.044
0.188GlnCys: 0.188 ± 0.008
1.435GlnAsp: 1.435 ± 0.023
1.452GlnGlu: 1.452 ± 0.026
0.689GlnPhe: 0.689 ± 0.016
2.368GlnGly: 2.368 ± 0.031
0.681GlnHis: 0.681 ± 0.016
1.071GlnIle: 1.071 ± 0.019
0.641GlnLys: 0.641 ± 0.017
3.054GlnLeu: 3.054 ± 0.037
0.491GlnMet: 0.491 ± 0.014
0.505GlnAsn: 0.505 ± 0.015
1.7GlnPro: 1.7 ± 0.034
1.283GlnGln: 1.283 ± 0.03
2.356GlnArg: 2.356 ± 0.03
1.316GlnSer: 1.316 ± 0.023
1.416GlnThr: 1.416 ± 0.023
2.394GlnVal: 2.394 ± 0.034
0.482GlnTrp: 0.482 ± 0.014
0.647GlnTyr: 0.647 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
9.969ArgAla: 9.969 ± 0.081
0.589ArgCys: 0.589 ± 0.015
4.212ArgAsp: 4.212 ± 0.045
4.71ArgGlu: 4.71 ± 0.045
2.327ArgPhe: 2.327 ± 0.028
5.678ArgGly: 5.678 ± 0.05
2.249ArgHis: 2.249 ± 0.035
3.167ArgIle: 3.167 ± 0.035
1.593ArgLys: 1.593 ± 0.027
9.18ArgLeu: 9.18 ± 0.073
1.726ArgMet: 1.726 ± 0.023
1.298ArgAsn: 1.298 ± 0.024
5.032ArgPro: 5.032 ± 0.046
2.4ArgGln: 2.4 ± 0.034
7.893ArgArg: 7.893 ± 0.07
3.959ArgSer: 3.959 ± 0.038
5.505ArgThr: 5.505 ± 0.049
5.92ArgVal: 5.92 ± 0.048
1.397ArgTrp: 1.397 ± 0.022
1.817ArgTyr: 1.817 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
6.892SerAla: 6.892 ± 0.061
0.423SerCys: 0.423 ± 0.013
2.71SerAsp: 2.71 ± 0.033
2.288SerGlu: 2.288 ± 0.031
1.494SerPhe: 1.494 ± 0.024
5.989SerGly: 5.989 ± 0.051
1.016SerHis: 1.016 ± 0.02
1.377SerIle: 1.377 ± 0.024
1.06SerLys: 1.06 ± 0.021
4.832SerLeu: 4.832 ± 0.043
1.078SerMet: 1.078 ± 0.022
0.888SerAsn: 0.888 ± 0.02
3.17SerPro: 3.17 ± 0.039
1.269SerGln: 1.269 ± 0.023
3.631SerArg: 3.631 ± 0.037
2.979SerSer: 2.979 ± 0.045
3.159SerThr: 3.159 ± 0.035
4.34SerVal: 4.34 ± 0.04
0.918SerTrp: 0.918 ± 0.023
1.297SerTyr: 1.297 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
9.141ThrAla: 9.141 ± 0.071
0.451ThrCys: 0.451 ± 0.015
3.711ThrAsp: 3.711 ± 0.04
3.112ThrGlu: 3.112 ± 0.034
1.67ThrPhe: 1.67 ± 0.029
6.728ThrGly: 6.728 ± 0.051
1.284ThrHis: 1.284 ± 0.021
1.666ThrIle: 1.666 ± 0.029
1.212ThrLys: 1.212 ± 0.024
5.858ThrLeu: 5.858 ± 0.053
0.939ThrMet: 0.939 ± 0.019
1.03ThrAsn: 1.03 ± 0.021
4.166ThrPro: 4.166 ± 0.044
1.429ThrGln: 1.429 ± 0.024
3.981ThrArg: 3.981 ± 0.045
3.303ThrSer: 3.303 ± 0.039
3.964ThrThr: 3.964 ± 0.043
6.301ThrVal: 6.301 ± 0.051
0.973ThrTrp: 0.973 ± 0.02
1.4ThrTyr: 1.4 ± 0.022
0.0ThrXaa: 0.0 ± 0.0
Val
10.756ValAla: 10.756 ± 0.073
0.774ValCys: 0.774 ± 0.018
5.026ValAsp: 5.026 ± 0.042
4.631ValGlu: 4.631 ± 0.049
2.479ValPhe: 2.479 ± 0.036
6.551ValGly: 6.551 ± 0.055
2.002ValHis: 2.002 ± 0.028
2.746ValIle: 2.746 ± 0.04
1.741ValLys: 1.741 ± 0.029
9.704ValLeu: 9.704 ± 0.073
1.424ValMet: 1.424 ± 0.024
1.668ValAsn: 1.668 ± 0.026
5.283ValPro: 5.283 ± 0.047
2.111ValGln: 2.111 ± 0.03
7.407ValArg: 7.407 ± 0.054
4.327ValSer: 4.327 ± 0.042
5.831ValThr: 5.831 ± 0.052
8.129ValVal: 8.129 ± 0.067
1.207ValTrp: 1.207 ± 0.023
1.643ValTyr: 1.643 ± 0.023
0.0ValXaa: 0.0 ± 0.0
Trp
1.729TrpAla: 1.729 ± 0.025
0.159TrpCys: 0.159 ± 0.008
0.85TrpAsp: 0.85 ± 0.018
0.707TrpGlu: 0.707 ± 0.019
0.514TrpPhe: 0.514 ± 0.014
1.097TrpGly: 1.097 ± 0.023
0.397TrpHis: 0.397 ± 0.012
0.587TrpIle: 0.587 ± 0.014
0.381TrpLys: 0.381 ± 0.012
1.831TrpLeu: 1.831 ± 0.026
0.311TrpMet: 0.311 ± 0.011
0.439TrpAsn: 0.439 ± 0.014
0.812TrpPro: 0.812 ± 0.017
0.645TrpGln: 0.645 ± 0.015
1.37TrpArg: 1.37 ± 0.025
0.993TrpSer: 0.993 ± 0.022
1.077TrpThr: 1.077 ± 0.021
0.987TrpVal: 0.987 ± 0.02
0.356TrpTrp: 0.356 ± 0.011
0.405TrpTyr: 0.405 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.848TyrAla: 2.848 ± 0.035
0.182TyrCys: 0.182 ± 0.009
1.616TyrAsp: 1.616 ± 0.029
1.275TyrGlu: 1.275 ± 0.022
0.658TyrPhe: 0.658 ± 0.016
2.366TyrGly: 2.366 ± 0.033
0.408TyrHis: 0.408 ± 0.012
0.499TyrIle: 0.499 ± 0.014
0.432TyrLys: 0.432 ± 0.014
2.111TyrLeu: 2.111 ± 0.029
0.269TyrMet: 0.269 ± 0.01
0.44TyrAsn: 0.44 ± 0.013
1.076TyrPro: 1.076 ± 0.022
0.611TyrGln: 0.611 ± 0.014
1.853TyrArg: 1.853 ± 0.028
0.991TyrSer: 0.991 ± 0.019
1.222TyrThr: 1.222 ± 0.024
1.734TyrVal: 1.734 ± 0.023
0.371TyrTrp: 0.371 ± 0.013
0.472TyrTyr: 0.472 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8721 proteins (2818862 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski