Fibrobacter sp. UWEL

Taxonomy: cellular organisms; Bacteria; FCB group; Fibrobacteres; Fibrobacteria; Fibrobacterales; Fibrobacteraceae; Fibrobacter; unclassified Fibrobacter

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2928 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6SFG6|A0A1M6SFG6_9BACT LexA repressor OS=Fibrobacter sp. UWEL OX=1896209 GN=lexA PE=3 SV=1
MM1 pKa = 7.7SYY3 pKa = 10.59NYY5 pKa = 10.16HH6 pKa = 7.03DD7 pKa = 5.65AIKK10 pKa = 9.71EE11 pKa = 4.03DD12 pKa = 4.27CEE14 pKa = 4.31TAIKK18 pKa = 10.31EE19 pKa = 4.01YY20 pKa = 10.9LDD22 pKa = 3.5YY23 pKa = 11.43HH24 pKa = 7.38KK25 pKa = 11.18DD26 pKa = 3.45EE27 pKa = 4.5VKK29 pKa = 10.79GMSKK33 pKa = 8.71EE34 pKa = 4.04TLTEE38 pKa = 4.02KK39 pKa = 10.54FHH41 pKa = 7.02DD42 pKa = 4.65AFWIDD47 pKa = 4.7DD48 pKa = 4.11SVTGNASGSYY58 pKa = 9.05TFSSYY63 pKa = 9.62EE64 pKa = 3.83AEE66 pKa = 4.14QNLAGNWDD74 pKa = 4.03LLGEE78 pKa = 4.28AMSEE82 pKa = 4.31FCCEE86 pKa = 3.65CDD88 pKa = 4.46AIAKK92 pKa = 8.26GAEE95 pKa = 4.03WADD98 pKa = 3.39VTIRR102 pKa = 11.84CYY104 pKa = 11.04LLDD107 pKa = 4.02EE108 pKa = 5.33GIEE111 pKa = 4.02KK112 pKa = 10.68AMEE115 pKa = 4.07EE116 pKa = 4.31LEE118 pKa = 4.15EE119 pKa = 5.59LIDD122 pKa = 3.82SAIEE126 pKa = 3.82EE127 pKa = 4.5SEE129 pKa = 4.22EE130 pKa = 3.89EE131 pKa = 4.37PEE133 pKa = 3.99EE134 pKa = 4.66SEE136 pKa = 4.46AA137 pKa = 4.93

Molecular weight: 15.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M7ARS9|A0A1M7ARS9_9BACT 50S ribosomal protein L3 OS=Fibrobacter sp. UWEL OX=1896209 GN=rplC PE=3 SV=1
MM1 pKa = 7.46ASKK4 pKa = 10.84RR5 pKa = 11.84MIEE8 pKa = 3.95KK9 pKa = 10.41CKK11 pKa = 9.75RR12 pKa = 11.84TPKK15 pKa = 9.04YY16 pKa = 8.5TVRR19 pKa = 11.84GYY21 pKa = 10.37NRR23 pKa = 11.84CKK25 pKa = 10.15RR26 pKa = 11.84CGRR29 pKa = 11.84PHH31 pKa = 7.62AFMRR35 pKa = 11.84RR36 pKa = 11.84FGLCRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.78MALAGEE52 pKa = 4.53IPGITKK58 pKa = 10.48SSWW61 pKa = 2.78

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2928

0

2928

1075629

26

6068

367.4

40.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.42 ± 0.052

1.333 ± 0.021

6.024 ± 0.046

6.507 ± 0.039

4.702 ± 0.03

7.27 ± 0.045

1.726 ± 0.02

5.897 ± 0.038

6.642 ± 0.051

8.76 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.02

4.683 ± 0.029

3.85 ± 0.029

3.063 ± 0.024

4.377 ± 0.037

6.759 ± 0.054

5.288 ± 0.039

7.113 ± 0.036

1.24 ± 0.016

3.639 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski