Tomato yellow leaf curl virus - Mild

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato yellow leaf curl virus

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q88943|Q88943_9GEMI Transcriptional activator protein OS=Tomato yellow leaf curl virus - Mild OX=220944 PE=3 SV=1
MM1 pKa = 7.71APPKK5 pKa = 10.19RR6 pKa = 11.84FQINCKK12 pKa = 10.27NYY14 pKa = 8.62FLTYY18 pKa = 7.84PHH20 pKa = 7.2CSLTNEE26 pKa = 4.19EE27 pKa = 5.1ALSQLKK33 pKa = 10.25NLEE36 pKa = 4.01TPTNKK41 pKa = 10.13KK42 pKa = 9.34YY43 pKa = 10.81IKK45 pKa = 10.03VCRR48 pKa = 11.84EE49 pKa = 3.48LHH51 pKa = 6.31EE52 pKa = 4.82NGEE55 pKa = 4.28PHH57 pKa = 6.66LHH59 pKa = 6.03VLIQFEE65 pKa = 5.24GKK67 pKa = 10.04FKK69 pKa = 10.78CQNQRR74 pKa = 11.84FFDD77 pKa = 3.95LVSPGRR83 pKa = 11.84AAHH86 pKa = 6.01FHH88 pKa = 6.69PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.1VKK101 pKa = 11.22SYY103 pKa = 11.11VDD105 pKa = 3.73KK106 pKa = 11.65DD107 pKa = 3.25GDD109 pKa = 3.98TVDD112 pKa = 3.07WGEE115 pKa = 3.79FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQSANDD132 pKa = 3.81AYY134 pKa = 10.72AAALNSGSKK143 pKa = 10.24SEE145 pKa = 3.97ALRR148 pKa = 11.84IIKK151 pKa = 9.9EE152 pKa = 4.3LVPKK156 pKa = 10.43DD157 pKa = 4.13YY158 pKa = 10.83ILQFHH163 pKa = 6.68NLNSNLDD170 pKa = 4.08RR171 pKa = 11.84IFQEE175 pKa = 4.33PPAPYY180 pKa = 9.57ISPFLSSSFNQVPDD194 pKa = 3.69EE195 pKa = 4.38LEE197 pKa = 3.9VWVSEE202 pKa = 4.02NVMSSAARR210 pKa = 11.84PWRR213 pKa = 11.84PNSIIIEE220 pKa = 4.33GDD222 pKa = 3.13SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.26YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTSSYY311 pKa = 10.83RR312 pKa = 11.84EE313 pKa = 3.96YY314 pKa = 11.26LDD316 pKa = 3.39EE317 pKa = 4.86EE318 pKa = 4.64KK319 pKa = 11.05NISLKK324 pKa = 10.6NWALKK329 pKa = 10.38NATFVTLYY337 pKa = 10.04EE338 pKa = 4.24PLFASINQGPTQDD351 pKa = 3.72SQEE354 pKa = 4.24EE355 pKa = 4.36TNKK358 pKa = 10.73AA359 pKa = 3.23

Molecular weight: 40.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q88942|Q88942_9GEMI Replication-associated protein (Fragment) OS=Tomato yellow leaf curl virus - Mild OX=220944 PE=3 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.44RR4 pKa = 11.84PGDD7 pKa = 3.95IIISTPVSKK16 pKa = 10.56VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.07SPYY27 pKa = 10.0SSRR30 pKa = 11.84AAVPIVQGTNKK41 pKa = 9.27RR42 pKa = 11.84RR43 pKa = 11.84SWTYY47 pKa = 9.3RR48 pKa = 11.84PMYY51 pKa = 9.36RR52 pKa = 11.84KK53 pKa = 9.08PRR55 pKa = 11.84IYY57 pKa = 10.75RR58 pKa = 11.84MYY60 pKa = 10.32RR61 pKa = 11.84SPDD64 pKa = 3.21VPRR67 pKa = 11.84GCEE70 pKa = 4.34GPCKK74 pKa = 9.32VQSYY78 pKa = 7.04EE79 pKa = 3.67QRR81 pKa = 11.84DD82 pKa = 4.2DD83 pKa = 3.51IKK85 pKa = 9.71HH86 pKa = 5.35TGIVRR91 pKa = 11.84CVSDD95 pKa = 3.31VTRR98 pKa = 11.84GSGITHH104 pKa = 6.35RR105 pKa = 11.84VGKK108 pKa = 9.5RR109 pKa = 11.84FCVKK113 pKa = 10.37SIYY116 pKa = 10.32FLGKK120 pKa = 9.15VWMDD124 pKa = 3.41EE125 pKa = 4.15NIKK128 pKa = 10.04KK129 pKa = 9.97QNHH132 pKa = 4.95TNQVMFFLVRR142 pKa = 11.84DD143 pKa = 3.63RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 10.91GNSPMDD153 pKa = 3.85FGQVFNMFDD162 pKa = 3.99NEE164 pKa = 4.11PSTATVKK171 pKa = 10.85NDD173 pKa = 2.82LRR175 pKa = 11.84DD176 pKa = 3.36RR177 pKa = 11.84FQVMRR182 pKa = 11.84KK183 pKa = 7.65FHH185 pKa = 6.03ATVIGGPSGMKK196 pKa = 9.5EE197 pKa = 3.54QALVKK202 pKa = 10.34RR203 pKa = 11.84FFKK206 pKa = 10.71INSHH210 pKa = 4.14VTYY213 pKa = 10.54NHH215 pKa = 5.79QEE217 pKa = 3.49AAKK220 pKa = 10.4YY221 pKa = 9.25EE222 pKa = 4.13NHH224 pKa = 6.46TEE226 pKa = 3.99NALLLYY232 pKa = 7.29MACTHH237 pKa = 7.07ASNPVYY243 pKa = 10.08ATMKK247 pKa = 9.61IRR249 pKa = 11.84IYY251 pKa = 10.55FYY253 pKa = 11.2DD254 pKa = 4.06SISNN258 pKa = 3.66

Molecular weight: 30.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1102

100

359

183.7

21.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.537 ± 0.648

2.087 ± 0.416

5.082 ± 0.384

4.628 ± 0.713

4.537 ± 0.392

4.9 ± 0.421

4.265 ± 0.779

6.443 ± 0.803

5.989 ± 0.585

6.624 ± 1.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.617

6.261 ± 0.527

5.898 ± 0.46

5.354 ± 0.746

6.352 ± 1.191

8.621 ± 1.389

5.082 ± 0.695

5.626 ± 0.929

1.361 ± 0.241

3.902 ± 0.501
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski