Clostridium sp. CAG:75

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2286 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5T3J6|R5T3J6_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:75 OX=1262836 GN=BN771_00442 PE=4 SV=1
MM1 pKa = 7.3LHH3 pKa = 6.87GGTDD7 pKa = 3.72LSVSXXIYY15 pKa = 9.99QYY17 pKa = 8.45QVRR20 pKa = 11.84QTTVDD25 pKa = 3.05TFEE28 pKa = 5.15IFMATDD34 pKa = 4.09GEE36 pKa = 4.3PEE38 pKa = 4.08EE39 pKa = 4.76IEE41 pKa = 4.61GLFCEE46 pKa = 5.75FIRR49 pKa = 11.84DD50 pKa = 3.94SLVGEE55 pKa = 3.91AAFLFRR61 pKa = 11.84YY62 pKa = 9.68YY63 pKa = 10.59EE64 pKa = 3.76QLLPDD69 pKa = 4.26EE70 pKa = 4.79LTGKK74 pKa = 10.48LKK76 pKa = 10.38FFWCMIPEE84 pKa = 3.93QTGDD88 pKa = 3.63VEE90 pKa = 5.22SVADD94 pKa = 3.54QQ95 pKa = 3.41

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5T1E0|R5T1E0_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:75 OX=1262836 GN=BN771_01355 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.02KK10 pKa = 9.86SRR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.65GFRR19 pKa = 11.84KK20 pKa = 10.11RR21 pKa = 11.84MQTANGRR28 pKa = 11.84KK29 pKa = 8.71VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.26GRR39 pKa = 11.84HH40 pKa = 5.5KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2286

0

2286

692856

30

1729

303.1

34.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.505 ± 0.057

1.492 ± 0.022

5.806 ± 0.047

7.099 ± 0.062

3.818 ± 0.039

6.802 ± 0.045

1.882 ± 0.023

7.224 ± 0.054

7.191 ± 0.062

8.651 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.16 ± 0.028

4.218 ± 0.038

3.121 ± 0.028

3.85 ± 0.039

4.429 ± 0.049

5.874 ± 0.056

5.657 ± 0.044

7.056 ± 0.045

0.868 ± 0.019

4.265 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski