Anaerosphaera aminiphila DSM 21120

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Anaerosphaera; Anaerosphaera aminiphila

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1915 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5P9P3|A0A1M5P9P3_9FIRM Putative rRNA methylase OS=Anaerosphaera aminiphila DSM 21120 OX=1120995 GN=SAMN02745245_00218 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.45LDD4 pKa = 4.15IKK6 pKa = 11.13DD7 pKa = 4.0FLQSPDD13 pKa = 3.15YY14 pKa = 11.52SMEE17 pKa = 4.03FEE19 pKa = 4.35GQLIEE24 pKa = 4.45QNSHH28 pKa = 6.17YY29 pKa = 10.92DD30 pKa = 3.28IDD32 pKa = 4.33DD33 pKa = 4.49LNLIMPIDD41 pKa = 3.76YY42 pKa = 10.8SGTVFNLGNEE52 pKa = 4.1ILLDD56 pKa = 3.64LCVKK60 pKa = 10.36YY61 pKa = 10.43KK62 pKa = 11.26YY63 pKa = 8.73NTQCDD68 pKa = 3.58RR69 pKa = 11.84CLKK72 pKa = 10.13PMVEE76 pKa = 3.86IVEE79 pKa = 4.62TNLEE83 pKa = 3.74AHH85 pKa = 6.43FFKK88 pKa = 10.97NLEE91 pKa = 4.1EE92 pKa = 4.19VFEE95 pKa = 4.9DD96 pKa = 4.04EE97 pKa = 5.39ASIEE101 pKa = 4.08YY102 pKa = 10.58FEE104 pKa = 6.26LEE106 pKa = 4.15DD107 pKa = 3.87SQIFLDD113 pKa = 5.32DD114 pKa = 5.24LIISQIITSIPFKK127 pKa = 10.56SLCDD131 pKa = 3.79EE132 pKa = 4.19NCKK135 pKa = 10.57GICPKK140 pKa = 10.47CGKK143 pKa = 9.43NLNEE147 pKa = 4.44GSCNCNEE154 pKa = 4.35EE155 pKa = 5.01LEE157 pKa = 4.16IDD159 pKa = 3.67PRR161 pKa = 11.84LGKK164 pKa = 10.84LMDD167 pKa = 4.62LFNDD171 pKa = 3.8EE172 pKa = 4.45EE173 pKa = 4.54VV174 pKa = 3.23

Molecular weight: 20.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5SF01|A0A1M5SF01_9FIRM Subtilase family protein OS=Anaerosphaera aminiphila DSM 21120 OX=1120995 GN=SAMN02745245_01171 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.31NKK10 pKa = 8.92QRR12 pKa = 11.84KK13 pKa = 7.85RR14 pKa = 11.84EE15 pKa = 3.9HH16 pKa = 6.14GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84SIIKK32 pKa = 9.78ARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.17GRR39 pKa = 11.84KK40 pKa = 8.67ILSAA44 pKa = 4.02

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1915

0

1915

608245

42

2547

317.6

35.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.567 ± 0.053

0.849 ± 0.021

5.775 ± 0.045

8.041 ± 0.067

4.521 ± 0.039

6.478 ± 0.049

1.235 ± 0.021

9.395 ± 0.066

8.474 ± 0.052

9.311 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.028

6.022 ± 0.046

2.863 ± 0.029

2.093 ± 0.033

3.439 ± 0.035

6.822 ± 0.045

5.054 ± 0.047

6.762 ± 0.043

0.603 ± 0.015

4.131 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski