Staphylococcus phage IME1318_01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; unclassified Azeredovirinae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JNL4|A0A1W6JNL4_9CAUD Capsid and scaffold protein OS=Staphylococcus phage IME1318_01 OX=1965486 PE=4 SV=1
MM1 pKa = 7.16RR2 pKa = 11.84QSVNLQLFNYY12 pKa = 9.63LYY14 pKa = 10.72TKK16 pKa = 10.58FEE18 pKa = 4.15EE19 pKa = 4.53LGVPIIRR26 pKa = 11.84TSEE29 pKa = 3.87LNQALPYY36 pKa = 9.96PFIAIQSIRR45 pKa = 11.84DD46 pKa = 3.96DD47 pKa = 3.34ISRR50 pKa = 11.84LTFDD54 pKa = 4.47SYY56 pKa = 11.69GGSPTAIIHH65 pKa = 5.51IWCTEE70 pKa = 3.88DD71 pKa = 5.35DD72 pKa = 3.63KK73 pKa = 12.06GKK75 pKa = 10.32NDD77 pKa = 3.4EE78 pKa = 5.54LYY80 pKa = 10.43IQVQSILLDD89 pKa = 4.41EE90 pKa = 5.09IEE92 pKa = 4.42LDD94 pKa = 4.18GYY96 pKa = 9.24TLTLPQISVNEE107 pKa = 4.14STEE110 pKa = 3.56QDD112 pKa = 3.37TNQTLSHH119 pKa = 5.09TTISVEE125 pKa = 4.34YY126 pKa = 10.0ASHH129 pKa = 6.55

Molecular weight: 14.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JNK7|A0A1W6JNK7_9CAUD Uncharacterized protein OS=Staphylococcus phage IME1318_01 OX=1965486 PE=4 SV=1
MM1 pKa = 7.4YY2 pKa = 10.0YY3 pKa = 10.68SKK5 pKa = 11.42GGTNEE10 pKa = 3.49MNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84NKK18 pKa = 10.01KK19 pKa = 8.78EE20 pKa = 3.64RR21 pKa = 11.84QQEE24 pKa = 3.93LTIWLMVASLIIQIIALIKK43 pKa = 9.91TFFF46 pKa = 4.01

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

13946

39

1257

193.7

22.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.736 ± 0.518

0.588 ± 0.096

6.669 ± 0.315

7.178 ± 0.47

3.786 ± 0.169

5.579 ± 0.182

1.836 ± 0.138

7.393 ± 0.302

8.712 ± 0.42

7.113 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.61 ± 0.133

7.006 ± 0.309

2.725 ± 0.232

4.159 ± 0.271

4.474 ± 0.307

6.052 ± 0.317

6.339 ± 0.24

6.296 ± 0.375

1.262 ± 0.12

4.489 ± 0.374
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski