Amino acid dipepetide frequency for Microbacterium maritypicum MF109

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.235AlaAla: 20.235 ± 0.244	0.626AlaCys: 0.626 ± 0.027	8.422AlaAsp: 8.422 ± 0.094	8.342AlaGlu: 8.342 ± 0.102	4.104AlaPhe: 4.104 ± 0.068	11.767AlaGly: 11.767 ± 0.106	2.502AlaHis: 2.502 ± 0.046	6.224AlaIle: 6.224 ± 0.082	2.723AlaLys: 2.723 ± 0.061	14.299AlaLeu: 14.299 ± 0.14	2.691AlaMet: 2.691 ± 0.05	2.301AlaAsn: 2.301 ± 0.051	6.655AlaPro: 6.655 ± 0.101	3.964AlaGln: 3.964 ± 0.059	8.766AlaArg: 8.766 ± 0.108	7.371AlaSer: 7.371 ± 0.095	7.501AlaThr: 7.501 ± 0.105	11.861AlaVal: 11.861 ± 0.136	1.839AlaTrp: 1.839 ± 0.047	2.381AlaTyr: 2.381 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.676CysAla: 0.676 ± 0.025	0.043CysCys: 0.043 ± 0.007	0.297CysAsp: 0.297 ± 0.014	0.245CysGlu: 0.245 ± 0.015	0.179CysPhe: 0.179 ± 0.01	0.529CysGly: 0.529 ± 0.02	0.112CysHis: 0.112 ± 0.01	0.214CysIle: 0.214 ± 0.013	0.055CysLys: 0.055 ± 0.007	0.422CysLeu: 0.422 ± 0.02	0.075CysMet: 0.075 ± 0.008	0.102CysAsn: 0.102 ± 0.009	0.263CysPro: 0.263 ± 0.017	0.101CysGln: 0.101 ± 0.008	0.328CysArg: 0.328 ± 0.016	0.266CysSer: 0.266 ± 0.015	0.329CysThr: 0.329 ± 0.02	0.42CysVal: 0.42 ± 0.02	0.062CysTrp: 0.062 ± 0.007	0.098CysTyr: 0.098 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	9.279AspAla: 9.279 ± 0.102	0.231AspCys: 0.231 ± 0.014	4.596AspAsp: 4.596 ± 0.068	4.514AspGlu: 4.514 ± 0.066	1.672AspPhe: 1.672 ± 0.036	6.217AspGly: 6.217 ± 0.074	1.134AspHis: 1.134 ± 0.029	2.647AspIle: 2.647 ± 0.05	1.033AspLys: 1.033 ± 0.034	6.2AspLeu: 6.2 ± 0.084	0.855AspMet: 0.855 ± 0.027	0.959AspAsn: 0.959 ± 0.03	4.179AspPro: 4.179 ± 0.064	1.56AspGln: 1.56 ± 0.039	4.513AspArg: 4.513 ± 0.072	2.686AspSer: 2.686 ± 0.046	2.967AspThr: 2.967 ± 0.054	5.476AspVal: 5.476 ± 0.072	0.985AspTrp: 0.985 ± 0.032	1.211AspTyr: 1.211 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	7.21GluAla: 7.21 ± 0.095	0.247GluCys: 0.247 ± 0.014	2.807GluAsp: 2.807 ± 0.052	3.201GluGlu: 3.201 ± 0.068	1.678GluPhe: 1.678 ± 0.036	4.352GluGly: 4.352 ± 0.062	1.389GluHis: 1.389 ± 0.036	2.971GluIle: 2.971 ± 0.053	1.563GluLys: 1.563 ± 0.038	6.239GluLeu: 6.239 ± 0.075	0.956GluMet: 0.956 ± 0.026	1.328GluAsn: 1.328 ± 0.035	2.868GluPro: 2.868 ± 0.055	2.218GluGln: 2.218 ± 0.042	5.051GluArg: 5.051 ± 0.084	3.106GluSer: 3.106 ± 0.05	3.18GluThr: 3.18 ± 0.053	4.661GluVal: 4.661 ± 0.063	0.917GluTrp: 0.917 ± 0.032	1.238GluTyr: 1.238 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	4.457PheAla: 4.457 ± 0.069	0.17PheCys: 0.17 ± 0.012	2.411PheAsp: 2.411 ± 0.043	1.716PheGlu: 1.716 ± 0.039	1.127PhePhe: 1.127 ± 0.039	3.428PheGly: 3.428 ± 0.056	0.551PheHis: 0.551 ± 0.024	1.246PheIle: 1.246 ± 0.034	0.42PheLys: 0.42 ± 0.019	3.036PheLeu: 3.036 ± 0.054	0.456PheMet: 0.456 ± 0.021	0.667PheAsn: 0.667 ± 0.024	1.446PhePro: 1.446 ± 0.035	0.822PheGln: 0.822 ± 0.028	1.901PheArg: 1.901 ± 0.046	1.698PheSer: 1.698 ± 0.038	2.229PheThr: 2.229 ± 0.049	2.819PheVal: 2.819 ± 0.051	0.506PheTrp: 0.506 ± 0.02	0.637PheTyr: 0.637 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	10.922GlyAla: 10.922 ± 0.113	0.533GlyCys: 0.533 ± 0.022	5.081GlyAsp: 5.081 ± 0.073	5.019GlyGlu: 5.019 ± 0.069	3.31GlyPhe: 3.31 ± 0.052	7.489GlyGly: 7.489 ± 0.1	1.707GlyHis: 1.707 ± 0.037	5.128GlyIle: 5.128 ± 0.065	2.057GlyLys: 2.057 ± 0.048	8.634GlyLeu: 8.634 ± 0.09	2.058GlyMet: 2.058 ± 0.045	1.668GlyAsn: 1.668 ± 0.045	3.548GlyPro: 3.548 ± 0.061	2.369GlyGln: 2.369 ± 0.048	6.047GlyArg: 6.047 ± 0.076	5.254GlySer: 5.254 ± 0.077	5.862GlyThr: 5.862 ± 0.095	8.008GlyVal: 8.008 ± 0.085	1.709GlyTrp: 1.709 ± 0.041	2.17GlyTyr: 2.17 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.357HisAla: 2.357 ± 0.049	0.095HisCys: 0.095 ± 0.009	1.362HisAsp: 1.362 ± 0.035	1.139HisGlu: 1.139 ± 0.027	0.564HisPhe: 0.564 ± 0.019	1.926HisGly: 1.926 ± 0.045	0.535HisHis: 0.535 ± 0.022	0.761HisIle: 0.761 ± 0.025	0.267HisLys: 0.267 ± 0.016	1.982HisLeu: 1.982 ± 0.046	0.311HisMet: 0.311 ± 0.018	0.324HisAsn: 0.324 ± 0.017	1.456HisPro: 1.456 ± 0.036	0.474HisGln: 0.474 ± 0.02	1.584HisArg: 1.584 ± 0.039	0.952HisSer: 0.952 ± 0.027	0.992HisThr: 0.992 ± 0.027	1.582HisVal: 1.582 ± 0.035	0.267HisTrp: 0.267 ± 0.018	0.385HisTyr: 0.385 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	7.772IleAla: 7.772 ± 0.075	0.238IleCys: 0.238 ± 0.015	3.841IleAsp: 3.841 ± 0.06	3.003IleGlu: 3.003 ± 0.046	1.266IlePhe: 1.266 ± 0.035	5.078IleGly: 5.078 ± 0.068	0.722IleHis: 0.722 ± 0.028	2.047IleIle: 2.047 ± 0.051	0.73IleLys: 0.73 ± 0.03	4.063IleLeu: 4.063 ± 0.063	0.69IleMet: 0.69 ± 0.025	0.907IleAsn: 0.907 ± 0.026	2.594IlePro: 2.594 ± 0.049	1.011IleGln: 1.011 ± 0.029	2.921IleArg: 2.921 ± 0.053	2.522IleSer: 2.522 ± 0.05	2.999IleThr: 2.999 ± 0.055	4.982IleVal: 4.982 ± 0.067	0.557IleTrp: 0.557 ± 0.023	0.764IleTyr: 0.764 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	2.48LysAla: 2.48 ± 0.058	0.059LysCys: 0.059 ± 0.007	1.196LysAsp: 1.196 ± 0.04	1.016LysGlu: 1.016 ± 0.036	0.497LysPhe: 0.497 ± 0.021	1.579LysGly: 1.579 ± 0.042	0.419LysHis: 0.419 ± 0.021	1.011LysIle: 1.011 ± 0.031	0.838LysLys: 0.838 ± 0.036	1.804LysLeu: 1.804 ± 0.036	0.405LysMet: 0.405 ± 0.018	0.529LysAsn: 0.529 ± 0.022	1.161LysPro: 1.161 ± 0.041	0.679LysGln: 0.679 ± 0.028	1.382LysArg: 1.382 ± 0.04	1.145LysSer: 1.145 ± 0.032	1.294LysThr: 1.294 ± 0.036	1.653LysVal: 1.653 ± 0.04	0.241LysTrp: 0.241 ± 0.014	0.432LysTyr: 0.432 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	14.235LeuAla: 14.235 ± 0.127	0.514LeuCys: 0.514 ± 0.023	6.709LeuAsp: 6.709 ± 0.082	4.919LeuGlu: 4.919 ± 0.069	3.014LeuPhe: 3.014 ± 0.064	9.109LeuGly: 9.109 ± 0.101	1.855LeuHis: 1.855 ± 0.04	4.934LeuIle: 4.934 ± 0.065	1.659LeuLys: 1.659 ± 0.047	10.298LeuLeu: 10.298 ± 0.141	1.729LeuMet: 1.729 ± 0.037	1.751LeuAsn: 1.751 ± 0.043	5.532LeuPro: 5.532 ± 0.069	2.57LeuGln: 2.57 ± 0.048	7.64LeuArg: 7.64 ± 0.102	5.967LeuSer: 5.967 ± 0.074	6.382LeuThr: 6.382 ± 0.07	9.035LeuVal: 9.035 ± 0.103	1.345LeuTrp: 1.345 ± 0.036	1.59LeuTyr: 1.59 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.091MetAla: 2.091 ± 0.043	0.093MetCys: 0.093 ± 0.009	0.848MetAsp: 0.848 ± 0.026	0.681MetGlu: 0.681 ± 0.022	0.591MetPhe: 0.591 ± 0.023	1.29MetGly: 1.29 ± 0.033	0.366MetHis: 0.366 ± 0.019	0.993MetIle: 0.993 ± 0.03	0.46MetLys: 0.46 ± 0.02	2.091MetLeu: 2.091 ± 0.049	0.392MetMet: 0.392 ± 0.02	0.531MetAsn: 0.531 ± 0.023	1.204MetPro: 1.204 ± 0.035	0.552MetGln: 0.552 ± 0.023	1.413MetArg: 1.413 ± 0.04	1.55MetSer: 1.55 ± 0.033	1.783MetThr: 1.783 ± 0.036	1.379MetVal: 1.379 ± 0.033	0.225MetTrp: 0.225 ± 0.013	0.279MetTyr: 0.279 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.556AsnAla: 2.556 ± 0.049	0.113AsnCys: 0.113 ± 0.01	1.224AsnAsp: 1.224 ± 0.038	0.965AsnGlu: 0.965 ± 0.029	0.585AsnPhe: 0.585 ± 0.022	1.925AsnGly: 1.925 ± 0.046	0.356AsnHis: 0.356 ± 0.018	0.852AsnIle: 0.852 ± 0.029	0.371AsnLys: 0.371 ± 0.021	1.821AsnLeu: 1.821 ± 0.036	0.313AsnMet: 0.313 ± 0.017	0.429AsnAsn: 0.429 ± 0.022	1.511AsnPro: 1.511 ± 0.034	0.54AsnGln: 0.54 ± 0.021	1.226AsnArg: 1.226 ± 0.034	1.023AsnSer: 1.023 ± 0.028	1.187AsnThr: 1.187 ± 0.037	1.63AsnVal: 1.63 ± 0.041	0.322AsnTrp: 0.322 ± 0.018	0.43AsnTyr: 0.43 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	6.88ProAla: 6.88 ± 0.089	0.192ProCys: 0.192 ± 0.013	3.708ProAsp: 3.708 ± 0.059	3.924ProGlu: 3.924 ± 0.062	1.766ProPhe: 1.766 ± 0.039	4.697ProGly: 4.697 ± 0.066	1.131ProHis: 1.131 ± 0.03	2.146ProIle: 2.146 ± 0.043	1.001ProLys: 1.001 ± 0.031	5.057ProLeu: 5.057 ± 0.065	0.902ProMet: 0.902 ± 0.027	0.971ProAsn: 0.971 ± 0.032	2.123ProPro: 2.123 ± 0.056	1.585ProGln: 1.585 ± 0.042	3.298ProArg: 3.298 ± 0.052	3.166ProSer: 3.166 ± 0.056	3.406ProThr: 3.406 ± 0.057	4.914ProVal: 4.914 ± 0.08	0.853ProTrp: 0.853 ± 0.028	1.018ProTyr: 1.018 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	3.527GlnAla: 3.527 ± 0.061	0.111GlnCys: 0.111 ± 0.01	1.307GlnAsp: 1.307 ± 0.035	1.427GlnGlu: 1.427 ± 0.036	0.862GlnPhe: 0.862 ± 0.029	2.105GlnGly: 2.105 ± 0.042	0.61GlnHis: 0.61 ± 0.022	1.439GlnIle: 1.439 ± 0.035	0.682GlnLys: 0.682 ± 0.023	3.063GlnLeu: 3.063 ± 0.06	0.541GlnMet: 0.541 ± 0.021	0.724GlnAsn: 0.724 ± 0.027	1.385GlnPro: 1.385 ± 0.036	1.141GlnGln: 1.141 ± 0.038	2.368GlnArg: 2.368 ± 0.054	1.444GlnSer: 1.444 ± 0.038	1.666GlnThr: 1.666 ± 0.037	2.367GlnVal: 2.367 ± 0.043	0.452GlnTrp: 0.452 ± 0.021	0.582GlnTyr: 0.582 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.76ArgAla: 8.76 ± 0.113	0.303ArgCys: 0.303 ± 0.016	4.282ArgAsp: 4.282 ± 0.062	4.349ArgGlu: 4.349 ± 0.064	2.37ArgPhe: 2.37 ± 0.042	5.467ArgGly: 5.467 ± 0.071	1.538ArgHis: 1.538 ± 0.034	3.912ArgIle: 3.912 ± 0.059	1.4ArgLys: 1.4 ± 0.037	7.061ArgLeu: 7.061 ± 0.1	1.862ArgMet: 1.862 ± 0.039	1.29ArgAsn: 1.29 ± 0.033	3.423ArgPro: 3.423 ± 0.057	1.953ArgGln: 1.953 ± 0.037	6.556ArgArg: 6.556 ± 0.101	4.11ArgSer: 4.11 ± 0.06	4.369ArgThr: 4.369 ± 0.059	5.789ArgVal: 5.789 ± 0.074	1.214ArgTrp: 1.214 ± 0.032	1.485ArgTyr: 1.485 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	7.362SerAla: 7.362 ± 0.088	0.263SerCys: 0.263 ± 0.016	3.247SerAsp: 3.247 ± 0.053	2.877SerGlu: 2.877 ± 0.056	1.968SerPhe: 1.968 ± 0.038	5.808SerGly: 5.808 ± 0.085	1.005SerHis: 1.005 ± 0.028	2.832SerIle: 2.832 ± 0.052	1.116SerLys: 1.116 ± 0.034	5.34SerLeu: 5.34 ± 0.079	1.328SerMet: 1.328 ± 0.031	1.07SerAsn: 1.07 ± 0.032	2.972SerPro: 2.972 ± 0.054	1.336SerGln: 1.336 ± 0.032	3.836SerArg: 3.836 ± 0.064	3.514SerSer: 3.514 ± 0.063	3.767SerThr: 3.767 ± 0.055	4.689SerVal: 4.689 ± 0.068	0.986SerTrp: 0.986 ± 0.029	1.108SerTyr: 1.108 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	8.268ThrAla: 8.268 ± 0.104	0.294ThrCys: 0.294 ± 0.018	3.721ThrAsp: 3.721 ± 0.06	3.151ThrGlu: 3.151 ± 0.055	1.957ThrPhe: 1.957 ± 0.042	5.794ThrGly: 5.794 ± 0.084	1.109ThrHis: 1.109 ± 0.036	3.058ThrIle: 3.058 ± 0.053	1.215ThrLys: 1.215 ± 0.037	6.149ThrLeu: 6.149 ± 0.07	1.066ThrMet: 1.066 ± 0.028	1.142ThrAsn: 1.142 ± 0.032	4.042ThrPro: 4.042 ± 0.066	1.54ThrGln: 1.54 ± 0.038	3.806ThrArg: 3.806 ± 0.057	3.482ThrSer: 3.482 ± 0.062	4.129ThrThr: 4.129 ± 0.075	5.913ThrVal: 5.913 ± 0.086	0.917ThrTrp: 0.917 ± 0.031	1.131ThrTyr: 1.131 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	11.431ValAla: 11.431 ± 0.112	0.479ValCys: 0.479 ± 0.02	5.706ValAsp: 5.706 ± 0.076	4.905ValGlu: 4.905 ± 0.078	2.942ValPhe: 2.942 ± 0.049	7.055ValGly: 7.055 ± 0.09	1.663ValHis: 1.663 ± 0.039	4.726ValIle: 4.726 ± 0.071	1.596ValLys: 1.596 ± 0.041	9.544ValLeu: 9.544 ± 0.1	1.538ValMet: 1.538 ± 0.038	1.792ValAsn: 1.792 ± 0.039	4.663ValPro: 4.663 ± 0.063	2.318ValGln: 2.318 ± 0.041	6.082ValArg: 6.082 ± 0.078	5.132ValSer: 5.132 ± 0.071	5.733ValThr: 5.733 ± 0.09	8.662ValVal: 8.662 ± 0.11	1.182ValTrp: 1.182 ± 0.034	1.469ValTyr: 1.469 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.658TrpAla: 1.658 ± 0.041	0.095TrpCys: 0.095 ± 0.008	0.834TrpAsp: 0.834 ± 0.027	0.719TrpGlu: 0.719 ± 0.026	0.617TrpPhe: 0.617 ± 0.026	1.148TrpGly: 1.148 ± 0.032	0.342TrpHis: 0.342 ± 0.018	0.817TrpIle: 0.817 ± 0.029	0.319TrpLys: 0.319 ± 0.016	1.699TrpLeu: 1.699 ± 0.04	0.363TrpMet: 0.363 ± 0.02	0.456TrpAsn: 0.456 ± 0.021	0.701TrpPro: 0.701 ± 0.027	0.55TrpGln: 0.55 ± 0.022	1.193TrpArg: 1.193 ± 0.035	0.952TrpSer: 0.952 ± 0.031	0.939TrpThr: 0.939 ± 0.029	1.193TrpVal: 1.193 ± 0.032	0.396TrpTrp: 0.396 ± 0.018	0.298TrpTyr: 0.298 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.419TyrAla: 2.419 ± 0.048	0.119TyrCys: 0.119 ± 0.01	1.316TyrAsp: 1.316 ± 0.038	1.067TyrGlu: 1.067 ± 0.026	0.674TyrPhe: 0.674 ± 0.027	1.812TyrGly: 1.812 ± 0.04	0.267TyrHis: 0.267 ± 0.018	0.749TyrIle: 0.749 ± 0.027	0.314TyrLys: 0.314 ± 0.016	1.983TyrLeu: 1.983 ± 0.043	0.283TyrMet: 0.283 ± 0.014	0.439TyrAsn: 0.439 ± 0.018	1.01TyrPro: 1.01 ± 0.029	0.52TyrGln: 0.52 ± 0.023	1.581TyrArg: 1.581 ± 0.038	1.085TyrSer: 1.085 ± 0.036	1.168TyrThr: 1.168 ± 0.034	1.585TyrVal: 1.585 ± 0.038	0.315TyrTrp: 0.315 ± 0.016	0.42TyrTyr: 0.42 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3856 proteins (1203482 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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