Halothiobacillus sp. XI15

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Halothiobacillaceae; Halothiobacillus; unclassified Halothiobacillus

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2012 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W1RTT8|A0A0W1RTT8_9GAMM HTH arsR-type domain-containing protein OS=Halothiobacillus sp. XI15 OX=1766620 GN=AUR63_02155 PE=4 SV=1
MM1 pKa = 7.62WAGQATAITIDD12 pKa = 3.65QKK14 pKa = 11.37PLIVQQALPPNVMLLLDD31 pKa = 4.8DD32 pKa = 4.4SGSMLRR38 pKa = 11.84EE39 pKa = 3.91YY40 pKa = 10.39MPDD43 pKa = 3.06ASYY46 pKa = 11.48LSDD49 pKa = 3.36TSDD52 pKa = 3.18QGMQSASVNYY62 pKa = 9.36IYY64 pKa = 10.98YY65 pKa = 9.93DD66 pKa = 3.37GSVTYY71 pKa = 9.1EE72 pKa = 4.16PPVKK76 pKa = 10.56ADD78 pKa = 3.03GTYY81 pKa = 10.84YY82 pKa = 11.03SDD84 pKa = 2.82ITTFPTLPLDD94 pKa = 4.22GFDD97 pKa = 4.46SSSSTADD104 pKa = 3.0VTRR107 pKa = 11.84YY108 pKa = 9.79YY109 pKa = 11.57SNGSYY114 pKa = 11.39ANVNFPFFRR123 pKa = 11.84AFCPPDD129 pKa = 3.35TTQSVVHH136 pKa = 6.29YY137 pKa = 8.95GDD139 pKa = 5.0CYY141 pKa = 10.81SATYY145 pKa = 10.71YY146 pKa = 11.27NDD148 pKa = 3.46ADD150 pKa = 4.0HH151 pKa = 6.93VFLFQDD157 pKa = 3.41SGNNLYY163 pKa = 10.71YY164 pKa = 10.83YY165 pKa = 10.23EE166 pKa = 5.66ADD168 pKa = 3.13ALAFVYY174 pKa = 7.51TTPDD178 pKa = 2.87GSGGYY183 pKa = 9.14IEE185 pKa = 5.89NYY187 pKa = 9.73VATNCADD194 pKa = 3.92LPTDD198 pKa = 3.94AQATCADD205 pKa = 4.27DD206 pKa = 5.38AATQQNVGNWFGYY219 pKa = 9.6YY220 pKa = 7.37RR221 pKa = 11.84TRR223 pKa = 11.84MLAAKK228 pKa = 9.48SGLMNGFADD237 pKa = 3.86VSAEE241 pKa = 3.61FRR243 pKa = 11.84VGFGSINGNSADD255 pKa = 3.96YY256 pKa = 10.51IRR258 pKa = 11.84NITAGEE264 pKa = 4.22CGDD267 pKa = 3.95VFSASCRR274 pKa = 11.84YY275 pKa = 10.45GFVTDD280 pKa = 3.62TNNNNEE286 pKa = 4.29LAGVATWGDD295 pKa = 3.52GAAGTRR301 pKa = 11.84KK302 pKa = 10.35DD303 pKa = 4.52EE304 pKa = 4.27FWGWLEE310 pKa = 5.33DD311 pKa = 4.55AGGDD315 pKa = 3.41QNTPLRR321 pKa = 11.84EE322 pKa = 3.94TLEE325 pKa = 4.34AAGEE329 pKa = 4.31YY330 pKa = 10.54YY331 pKa = 9.15RR332 pKa = 11.84TGQPWSSRR340 pKa = 11.84DD341 pKa = 3.41SSGTSQEE348 pKa = 4.28YY349 pKa = 10.63ACRR352 pKa = 11.84QSYY355 pKa = 8.66TILTSDD361 pKa = 3.77GFWNEE366 pKa = 3.65VGAPDD371 pKa = 4.82PDD373 pKa = 3.56VGNADD378 pKa = 3.76GTSGATLGDD387 pKa = 4.39DD388 pKa = 4.35GSGTMTAPNGIEE400 pKa = 3.89YY401 pKa = 8.75TYY403 pKa = 10.82EE404 pKa = 3.95AVDD407 pKa = 4.37PYY409 pKa = 11.56SDD411 pKa = 4.75DD412 pKa = 3.99FSNTLADD419 pKa = 3.12VAMYY423 pKa = 9.66YY424 pKa = 9.45WKK426 pKa = 10.59NDD428 pKa = 2.85LRR430 pKa = 11.84TGLTNQVPTTNEE442 pKa = 3.97DD443 pKa = 3.5PAFWQHH449 pKa = 5.0MTTFAIGLGYY459 pKa = 10.64DD460 pKa = 3.56PLDD463 pKa = 3.97ANGNVIDD470 pKa = 4.47SDD472 pKa = 5.32AVFDD476 pKa = 4.04WANGGDD482 pKa = 4.34PISGFAWPEE491 pKa = 3.73PASGSINNIADD502 pKa = 3.7MLHH505 pKa = 6.37AAVNGHH511 pKa = 6.08GGFYY515 pKa = 10.12SAKK518 pKa = 9.87SSQAFAAAISDD529 pKa = 3.76AVGRR533 pKa = 11.84TAEE536 pKa = 4.16RR537 pKa = 11.84VGTGASLAANSTRR550 pKa = 11.84LEE552 pKa = 4.4TGTVTYY558 pKa = 9.42QANYY562 pKa = 7.51FTGTWVGDD570 pKa = 3.61LKK572 pKa = 11.03AYY574 pKa = 9.68PVDD577 pKa = 3.98PLTKK581 pKa = 10.34QIATYY586 pKa = 9.32PDD588 pKa = 3.2WSAADD593 pKa = 3.63NLLAEE598 pKa = 4.93PADD601 pKa = 3.74SCAASAGEE609 pKa = 3.97VCPDD613 pKa = 2.49GRR615 pKa = 11.84AIYY618 pKa = 9.0TYY620 pKa = 9.74ATSDD624 pKa = 3.45ANLGNNDD631 pKa = 3.13PQYY634 pKa = 11.22RR635 pKa = 11.84EE636 pKa = 4.01FLASDD641 pKa = 3.63VASLSNAQQAALGGTTTEE659 pKa = 3.92RR660 pKa = 11.84QAIVDD665 pKa = 4.18FLRR668 pKa = 11.84GDD670 pKa = 3.62TTNAEE675 pKa = 4.44ANGGNYY681 pKa = 9.0RR682 pKa = 11.84DD683 pKa = 3.92RR684 pKa = 11.84STALGDD690 pKa = 3.53IVNSQPVYY698 pKa = 10.58VGQPDD703 pKa = 4.38PNLHH707 pKa = 5.5TARR710 pKa = 11.84SFDD713 pKa = 3.31GVEE716 pKa = 4.76FYY718 pKa = 10.43PAFAEE723 pKa = 5.2DD724 pKa = 4.37PEE726 pKa = 5.86GDD728 pKa = 4.05NDD730 pKa = 3.77NQGIDD735 pKa = 3.84PEE737 pKa = 4.93GGSNTRR743 pKa = 11.84EE744 pKa = 3.82PLVYY748 pKa = 10.24VAANDD753 pKa = 3.87GMLHH757 pKa = 6.27GFDD760 pKa = 5.48AITGEE765 pKa = 4.1EE766 pKa = 5.15LYY768 pKa = 11.1AFMPGAVIEE777 pKa = 4.53AGVKK781 pKa = 10.08KK782 pKa = 10.43LADD785 pKa = 4.02PDD787 pKa = 3.74YY788 pKa = 10.7GANPGHH794 pKa = 6.41EE795 pKa = 4.16FFNDD799 pKa = 3.76GEE801 pKa = 4.43LTVADD806 pKa = 4.38VYY808 pKa = 11.27IDD810 pKa = 3.88LPYY813 pKa = 10.94TNGSDD818 pKa = 3.91VQWRR822 pKa = 11.84TVLVGTTGRR831 pKa = 11.84GEE833 pKa = 4.28AKK835 pKa = 9.27TVYY838 pKa = 10.55ALDD841 pKa = 3.35ITDD844 pKa = 4.05PADD847 pKa = 4.47IRR849 pKa = 11.84FLWEE853 pKa = 3.7RR854 pKa = 11.84SAGDD858 pKa = 3.25GGANAGYY865 pKa = 9.15IGQMIGKK872 pKa = 8.34PLVVQTANGEE882 pKa = 4.05WSVLIGNGYY891 pKa = 10.46NSANDD896 pKa = 3.57SAALLQFDD904 pKa = 4.53VGTGALDD911 pKa = 3.31VHH913 pKa = 5.58VTNDD917 pKa = 4.0GITGNGLAAPATFDD931 pKa = 3.74YY932 pKa = 10.62TDD934 pKa = 4.22SPDD937 pKa = 4.2GIQDD941 pKa = 3.2VAYY944 pKa = 10.42AGDD947 pKa = 3.78LNGWVWRR954 pKa = 11.84FDD956 pKa = 3.73LSTSTSNGVQVYY968 pKa = 8.84QATRR972 pKa = 11.84ADD974 pKa = 3.75GTAQPITAGLLLGKK988 pKa = 10.19NPATSNLWAFFGTGQYY1004 pKa = 10.8LSQGDD1009 pKa = 4.08LTDD1012 pKa = 3.63QSVQTWYY1019 pKa = 11.25GLIVEE1024 pKa = 4.87SADD1027 pKa = 3.08TSLPVSSGDD1036 pKa = 3.33GRR1038 pKa = 11.84SVLVEE1043 pKa = 3.67RR1044 pKa = 11.84DD1045 pKa = 3.33IVDD1048 pKa = 3.57EE1049 pKa = 4.0QSNGTFTARR1058 pKa = 11.84AFSQGAANDD1067 pKa = 3.74MDD1069 pKa = 5.18GKK1071 pKa = 10.49SGWYY1075 pKa = 9.04IDD1077 pKa = 5.91LISPDD1082 pKa = 3.36NGAEE1086 pKa = 4.2GEE1088 pKa = 4.4RR1089 pKa = 11.84MVEE1092 pKa = 3.84PNQFQGEE1099 pKa = 4.59VLVGTSVIPDD1109 pKa = 3.42AADD1112 pKa = 2.84ICNPSGRR1119 pKa = 11.84GFIMGIDD1126 pKa = 3.82PFDD1129 pKa = 3.83GTNVEE1134 pKa = 4.29QAFVDD1139 pKa = 3.89YY1140 pKa = 11.29NGDD1143 pKa = 3.78GVVDD1147 pKa = 4.64GSDD1150 pKa = 3.98VITGDD1155 pKa = 4.05DD1156 pKa = 3.6GVTYY1160 pKa = 10.63AVGAIGFNRR1169 pKa = 11.84LPNAPIFVGNTMLISFDD1186 pKa = 4.27DD1187 pKa = 4.52GSTASVEE1194 pKa = 4.14AAGGGSSGQRR1204 pKa = 11.84SSWRR1208 pKa = 11.84EE1209 pKa = 3.57IIFDD1213 pKa = 3.63

Molecular weight: 128.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W1RSW3|A0A0W1RSW3_9GAMM DNA deoxyribophosphodiesterase OS=Halothiobacillus sp. XI15 OX=1766620 GN=AUR63_03360 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.16RR14 pKa = 11.84THH16 pKa = 6.07GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 9.32TRR25 pKa = 11.84GGRR28 pKa = 11.84AIINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2012

0

2012

676788

41

2201

336.4

36.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.436 ± 0.073

0.711 ± 0.016

6.417 ± 0.051

6.962 ± 0.055

3.449 ± 0.034

8.333 ± 0.054

2.312 ± 0.027

4.894 ± 0.04

2.613 ± 0.045

10.525 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.404 ± 0.025

2.505 ± 0.029

5.049 ± 0.038

3.519 ± 0.031

7.809 ± 0.054

5.033 ± 0.035

4.97 ± 0.038

7.424 ± 0.056

1.426 ± 0.023

2.21 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski