Tortoise microvirus 60

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6H6|A0A4P8W6H6_9VIRU Uncharacterized protein OS=Tortoise microvirus 60 OX=2583166 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.68NSIYY6 pKa = 10.44CLYY9 pKa = 10.5NVLSHH14 pKa = 7.2RR15 pKa = 11.84YY16 pKa = 8.89GDD18 pKa = 4.07VVCFPSDD25 pKa = 2.9AFAVQRR31 pKa = 11.84LRR33 pKa = 11.84EE34 pKa = 4.13SLKK37 pKa = 10.31PEE39 pKa = 3.69QLIEE43 pKa = 5.24LEE45 pKa = 4.37LCNLGYY51 pKa = 10.06IDD53 pKa = 5.01IEE55 pKa = 4.33SGMIEE60 pKa = 3.67THH62 pKa = 5.88APIRR66 pKa = 11.84IPIPQEE72 pKa = 3.71FSPVPIDD79 pKa = 4.75DD80 pKa = 3.92YY81 pKa = 11.9EE82 pKa = 4.11KK83 pKa = 11.2

Molecular weight: 9.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1FVZ7|A0A4V1FVZ7_9VIRU Replication initiation protein OS=Tortoise microvirus 60 OX=2583166 PE=4 SV=1
MM1 pKa = 7.31SCVNPVTIRR10 pKa = 11.84VNDD13 pKa = 3.66TNYY16 pKa = 10.77DD17 pKa = 3.82VPCRR21 pKa = 11.84RR22 pKa = 11.84CMSCRR27 pKa = 11.84LDD29 pKa = 3.47YY30 pKa = 10.76QIYY33 pKa = 8.15LTFCAEE39 pKa = 3.95NEE41 pKa = 4.21LYY43 pKa = 10.53DD44 pKa = 4.24CYY46 pKa = 10.94RR47 pKa = 11.84SGLGASFCTFTYY59 pKa = 10.73DD60 pKa = 4.83DD61 pKa = 3.95DD62 pKa = 5.53HH63 pKa = 8.62IPVNGSLCKK72 pKa = 9.8KK73 pKa = 10.16DD74 pKa = 3.57LQNFLKK80 pKa = 9.99TGRR83 pKa = 11.84KK84 pKa = 7.99NLEE87 pKa = 3.59RR88 pKa = 11.84SGFPASFKK96 pKa = 10.85YY97 pKa = 9.78LACGEE102 pKa = 4.35YY103 pKa = 10.45GDD105 pKa = 4.39KK106 pKa = 10.56FSRR109 pKa = 11.84PHH111 pKa = 4.56YY112 pKa = 9.0HH113 pKa = 5.69VAFIGLSDD121 pKa = 3.6VLVNEE126 pKa = 4.94FFRR129 pKa = 11.84PAWKK133 pKa = 10.01HH134 pKa = 4.57GLVDD138 pKa = 3.74IGVLQSGGMRR148 pKa = 11.84YY149 pKa = 8.92ILKK152 pKa = 9.7YY153 pKa = 8.28CTKK156 pKa = 10.69AVLGNKK162 pKa = 9.59AKK164 pKa = 10.38EE165 pKa = 4.07LYY167 pKa = 9.96DD168 pKa = 3.97DD169 pKa = 4.96KK170 pKa = 11.5GLEE173 pKa = 4.03RR174 pKa = 11.84PFITRR179 pKa = 11.84SCKK182 pKa = 9.24MGYY185 pKa = 9.96EE186 pKa = 3.79YY187 pKa = 10.83LYY189 pKa = 11.03RR190 pKa = 11.84NIDD193 pKa = 4.23DD194 pKa = 3.65IVANNFTYY202 pKa = 10.63LKK204 pKa = 10.55AGIRR208 pKa = 11.84RR209 pKa = 11.84PIPKK213 pKa = 9.87YY214 pKa = 10.74LRR216 pKa = 11.84DD217 pKa = 3.71KK218 pKa = 11.19FDD220 pKa = 3.35LFKK223 pKa = 11.26NYY225 pKa = 10.36DD226 pKa = 4.19SLPMILRR233 pKa = 11.84IKK235 pKa = 10.68AEE237 pKa = 4.04SDD239 pKa = 3.25LYY241 pKa = 11.13GYY243 pKa = 9.72PDD245 pKa = 3.34YY246 pKa = 11.18RR247 pKa = 11.84DD248 pKa = 3.04WQRR251 pKa = 11.84IKK253 pKa = 10.95NYY255 pKa = 9.62NRR257 pKa = 11.84EE258 pKa = 3.87QGLISAARR266 pKa = 11.84SKK268 pKa = 10.86GYY270 pKa = 9.53PVDD273 pKa = 4.36DD274 pKa = 3.49SSFRR278 pKa = 11.84LTARR282 pKa = 11.84SVSSSGKK289 pKa = 9.62EE290 pKa = 3.63LAKK293 pKa = 10.63LAFRR297 pKa = 11.84RR298 pKa = 11.84SS299 pKa = 3.33

Molecular weight: 34.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1723

68

579

246.1

27.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.125 ± 1.64

1.567 ± 0.85

7.197 ± 0.472

4.701 ± 0.977

5.92 ± 0.462

6.094 ± 0.574

1.277 ± 0.375

4.875 ± 0.507

4.411 ± 1.172

8.299 ± 0.789

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.96 ± 0.513

5.514 ± 0.468

4.295 ± 1.099

3.656 ± 0.897

5.63 ± 0.707

10.215 ± 1.195

4.643 ± 0.698

5.165 ± 0.714

0.638 ± 0.169

4.817 ± 0.963
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski