Nocardioides lianchengensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5063 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7B3E9|A0A1G7B3E9_9ACTN NAD kinase OS=Nocardioides lianchengensis OX=1045774 GN=nadK PE=3 SV=1
MM1 pKa = 7.8LLRR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 9.19IALAVCATAITAPVLSSCGFNYY28 pKa = 9.34ATDD31 pKa = 3.68QPYY34 pKa = 11.18DD35 pKa = 3.52PAAGTNNRR43 pKa = 11.84DD44 pKa = 3.05ARR46 pKa = 11.84VDD48 pKa = 3.4VLAAVVVAGQDD59 pKa = 3.46DD60 pKa = 4.43RR61 pKa = 11.84GTLVAGLANNDD72 pKa = 4.41DD73 pKa = 4.31EE74 pKa = 5.74PISLTSVVGEE84 pKa = 4.1GLTAEE89 pKa = 4.79FDD91 pKa = 4.63PIEE94 pKa = 4.51VPASGYY100 pKa = 11.03VNLADD105 pKa = 3.98EE106 pKa = 4.66EE107 pKa = 4.95VHH109 pKa = 7.0VEE111 pKa = 4.06GSFAAGDD118 pKa = 3.85VLKK121 pKa = 11.13LSLDD125 pKa = 3.61LDD127 pKa = 4.02NGEE130 pKa = 4.45SVALEE135 pKa = 4.1VPVVTNCDD143 pKa = 3.11EE144 pKa = 4.19FAGFDD149 pKa = 3.39TSTGEE154 pKa = 4.21GAEE157 pKa = 4.38SEE159 pKa = 4.37DD160 pKa = 3.43PAYY163 pKa = 10.29SCEE166 pKa = 4.13YY167 pKa = 10.4AEE169 pKa = 5.31HH170 pKa = 6.76EE171 pKa = 4.82GGHH174 pKa = 5.79

Molecular weight: 18.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6TG42|A0A1G6TG42_9ACTN Uncharacterized conserved protein YbjT contains NAD(P)-binding and DUF2867 domains OS=Nocardioides lianchengensis OX=1045774 GN=BKA20_003077 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5063

0

5063

1642176

29

3353

324.3

34.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.323 ± 0.047

0.669 ± 0.008

6.592 ± 0.032

5.648 ± 0.033

2.645 ± 0.018

9.334 ± 0.031

2.054 ± 0.018

3.09 ± 0.024

1.824 ± 0.027

10.401 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.652 ± 0.013

1.658 ± 0.018

5.688 ± 0.029

2.677 ± 0.018

7.69 ± 0.043

5.25 ± 0.027

6.434 ± 0.04

9.869 ± 0.033

1.518 ± 0.015

1.982 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski