Amino acid dipepetide frequency for Lactobacillus phage PM411

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.862AlaAla: 5.862 ± 1.275
0.234AlaCys: 0.234 ± 0.102
6.518AlaAsp: 6.518 ± 0.8
3.845AlaGlu: 3.845 ± 0.415
3.329AlaPhe: 3.329 ± 0.527
7.034AlaGly: 7.034 ± 0.578
1.407AlaHis: 1.407 ± 0.236
5.299AlaIle: 5.299 ± 0.44
6.753AlaLys: 6.753 ± 0.759
6.002AlaLeu: 6.002 ± 0.534
2.251AlaMet: 2.251 ± 0.431
4.924AlaAsn: 4.924 ± 0.572
2.86AlaPro: 2.86 ± 0.495
3.47AlaGln: 3.47 ± 0.458
1.876AlaArg: 1.876 ± 0.248
6.284AlaSer: 6.284 ± 0.717
7.972AlaThr: 7.972 ± 0.797
5.299AlaVal: 5.299 ± 0.412
0.938AlaTrp: 0.938 ± 0.281
2.767AlaTyr: 2.767 ± 0.325
0.0AlaXaa: 0.0 ± 0.0
Cys
0.141CysAla: 0.141 ± 0.07
0.094CysCys: 0.094 ± 0.058
0.188CysAsp: 0.188 ± 0.091
0.422CysGlu: 0.422 ± 0.145
0.234CysPhe: 0.234 ± 0.108
0.234CysGly: 0.234 ± 0.108
0.094CysHis: 0.094 ± 0.066
0.094CysIle: 0.094 ± 0.063
0.516CysLys: 0.516 ± 0.187
0.75CysLeu: 0.75 ± 0.198
0.0CysMet: 0.0 ± 0.0
0.094CysAsn: 0.094 ± 0.065
0.141CysPro: 0.141 ± 0.119
0.281CysGln: 0.281 ± 0.131
0.234CysArg: 0.234 ± 0.121
0.328CysSer: 0.328 ± 0.132
0.188CysThr: 0.188 ± 0.1
0.094CysVal: 0.094 ± 0.066
0.047CysTrp: 0.047 ± 0.044
0.234CysTyr: 0.234 ± 0.087
0.0CysXaa: 0.0 ± 0.0
Asp
4.924AspAla: 4.924 ± 0.603
0.422AspCys: 0.422 ± 0.152
6.096AspAsp: 6.096 ± 0.826
4.127AspGlu: 4.127 ± 0.52
3.329AspPhe: 3.329 ± 0.538
5.674AspGly: 5.674 ± 0.558
1.079AspHis: 1.079 ± 0.233
3.283AspIle: 3.283 ± 0.399
4.174AspLys: 4.174 ± 0.49
5.862AspLeu: 5.862 ± 0.45
1.735AspMet: 1.735 ± 0.374
3.001AspAsn: 3.001 ± 0.465
2.063AspPro: 2.063 ± 0.448
3.376AspGln: 3.376 ± 0.401
1.829AspArg: 1.829 ± 0.261
5.533AspSer: 5.533 ± 0.596
3.705AspThr: 3.705 ± 0.462
3.705AspVal: 3.705 ± 0.365
1.125AspTrp: 1.125 ± 0.2
2.673AspTyr: 2.673 ± 0.418
0.0AspXaa: 0.0 ± 0.0
Glu
3.798GluAla: 3.798 ± 0.305
0.516GluCys: 0.516 ± 0.181
3.189GluAsp: 3.189 ± 0.477
2.345GluGlu: 2.345 ± 0.391
1.923GluPhe: 1.923 ± 0.295
1.782GluGly: 1.782 ± 0.232
1.313GluHis: 1.313 ± 0.242
3.798GluIle: 3.798 ± 0.462
3.892GluLys: 3.892 ± 0.499
5.393GluLeu: 5.393 ± 0.59
1.782GluMet: 1.782 ± 0.227
2.814GluAsn: 2.814 ± 0.3
0.844GluPro: 0.844 ± 0.224
3.142GluGln: 3.142 ± 0.367
1.829GluArg: 1.829 ± 0.295
3.189GluSer: 3.189 ± 0.363
3.423GluThr: 3.423 ± 0.399
2.907GluVal: 2.907 ± 0.324
0.422GluTrp: 0.422 ± 0.124
1.923GluTyr: 1.923 ± 0.314
0.0GluXaa: 0.0 ± 0.0
Phe
3.236PheAla: 3.236 ± 0.466
0.094PheCys: 0.094 ± 0.066
3.189PheAsp: 3.189 ± 0.372
2.11PheGlu: 2.11 ± 0.362
1.313PhePhe: 1.313 ± 0.289
2.673PheGly: 2.673 ± 0.34
0.563PheHis: 0.563 ± 0.155
1.688PheIle: 1.688 ± 0.296
3.189PheLys: 3.189 ± 0.403
2.298PheLeu: 2.298 ± 0.404
0.75PheMet: 0.75 ± 0.191
2.72PheAsn: 2.72 ± 0.374
0.938PhePro: 0.938 ± 0.212
1.079PheGln: 1.079 ± 0.207
0.891PheArg: 0.891 ± 0.198
2.579PheSer: 2.579 ± 0.447
2.438PheThr: 2.438 ± 0.313
2.72PheVal: 2.72 ± 0.467
0.469PheTrp: 0.469 ± 0.146
1.688PheTyr: 1.688 ± 0.236
0.0PheXaa: 0.0 ± 0.0
Gly
4.455GlyAla: 4.455 ± 0.584
0.0GlyCys: 0.0 ± 0.0
4.08GlyAsp: 4.08 ± 0.441
3.564GlyGlu: 3.564 ± 0.356
3.048GlyPhe: 3.048 ± 0.406
4.549GlyGly: 4.549 ± 0.643
1.36GlyHis: 1.36 ± 0.214
3.705GlyIle: 3.705 ± 0.448
6.284GlyLys: 6.284 ± 0.714
6.096GlyLeu: 6.096 ± 0.578
2.016GlyMet: 2.016 ± 0.281
3.798GlyAsn: 3.798 ± 0.359
1.172GlyPro: 1.172 ± 0.285
2.673GlyGln: 2.673 ± 0.471
2.814GlyArg: 2.814 ± 0.329
5.018GlySer: 5.018 ± 0.504
5.627GlyThr: 5.627 ± 0.533
5.674GlyVal: 5.674 ± 0.509
1.172GlyTrp: 1.172 ± 0.246
3.236GlyTyr: 3.236 ± 0.409
0.0GlyXaa: 0.0 ± 0.0
His
1.125HisAla: 1.125 ± 0.254
0.094HisCys: 0.094 ± 0.068
1.125HisAsp: 1.125 ± 0.262
0.891HisGlu: 0.891 ± 0.199
0.657HisPhe: 0.657 ± 0.136
1.547HisGly: 1.547 ± 0.282
0.469HisHis: 0.469 ± 0.149
1.032HisIle: 1.032 ± 0.262
1.125HisLys: 1.125 ± 0.24
1.125HisLeu: 1.125 ± 0.241
0.188HisMet: 0.188 ± 0.1
1.125HisAsn: 1.125 ± 0.206
0.563HisPro: 0.563 ± 0.151
0.703HisGln: 0.703 ± 0.17
0.75HisArg: 0.75 ± 0.215
1.407HisSer: 1.407 ± 0.294
1.032HisThr: 1.032 ± 0.192
1.125HisVal: 1.125 ± 0.232
0.61HisTrp: 0.61 ± 0.161
0.797HisTyr: 0.797 ± 0.217
0.0HisXaa: 0.0 ± 0.0
Ile
5.487IleAla: 5.487 ± 0.567
0.516IleCys: 0.516 ± 0.187
5.205IleAsp: 5.205 ± 0.678
3.845IleGlu: 3.845 ± 0.379
2.11IlePhe: 2.11 ± 0.354
3.658IleGly: 3.658 ± 0.404
0.938IleHis: 0.938 ± 0.209
3.423IleIle: 3.423 ± 0.433
5.44IleLys: 5.44 ± 0.42
3.283IleLeu: 3.283 ± 0.35
1.125IleMet: 1.125 ± 0.271
4.361IleAsn: 4.361 ± 0.508
1.735IlePro: 1.735 ± 0.294
2.907IleGln: 2.907 ± 0.389
1.923IleArg: 1.923 ± 0.352
4.127IleSer: 4.127 ± 0.468
4.924IleThr: 4.924 ± 0.597
3.892IleVal: 3.892 ± 0.336
0.516IleTrp: 0.516 ± 0.134
2.345IleTyr: 2.345 ± 0.388
0.0IleXaa: 0.0 ± 0.0
Lys
6.846LysAla: 6.846 ± 1.042
0.188LysCys: 0.188 ± 0.099
4.127LysAsp: 4.127 ± 0.572
3.376LysGlu: 3.376 ± 0.404
2.626LysPhe: 2.626 ± 0.339
4.455LysGly: 4.455 ± 0.705
1.688LysHis: 1.688 ± 0.344
4.314LysIle: 4.314 ± 0.448
4.736LysLys: 4.736 ± 0.661
6.143LysLeu: 6.143 ± 0.406
2.485LysMet: 2.485 ± 0.362
3.517LysAsn: 3.517 ± 0.443
2.438LysPro: 2.438 ± 0.411
4.174LysGln: 4.174 ± 0.375
3.376LysArg: 3.376 ± 0.406
6.237LysSer: 6.237 ± 1.029
4.877LysThr: 4.877 ± 0.622
5.111LysVal: 5.111 ± 0.515
0.75LysTrp: 0.75 ± 0.156
2.814LysTyr: 2.814 ± 0.351
0.0LysXaa: 0.0 ± 0.0
Leu
7.175LeuAla: 7.175 ± 0.579
0.516LeuCys: 0.516 ± 0.151
5.487LeuAsp: 5.487 ± 0.504
4.08LeuGlu: 4.08 ± 0.483
2.204LeuPhe: 2.204 ± 0.416
5.299LeuGly: 5.299 ± 0.462
1.454LeuHis: 1.454 ± 0.229
5.487LeuIle: 5.487 ± 0.509
6.237LeuLys: 6.237 ± 0.527
4.642LeuLeu: 4.642 ± 0.501
1.688LeuMet: 1.688 ± 0.344
5.533LeuAsn: 5.533 ± 0.498
3.564LeuPro: 3.564 ± 0.496
3.47LeuGln: 3.47 ± 0.409
2.532LeuArg: 2.532 ± 0.435
5.815LeuSer: 5.815 ± 0.469
6.424LeuThr: 6.424 ± 0.534
4.22LeuVal: 4.22 ± 0.427
0.75LeuTrp: 0.75 ± 0.21
1.876LeuTyr: 1.876 ± 0.293
0.0LeuXaa: 0.0 ± 0.0
Met
2.673MetAla: 2.673 ± 0.393
0.0MetCys: 0.0 ± 0.0
1.313MetAsp: 1.313 ± 0.311
0.891MetGlu: 0.891 ± 0.185
0.75MetPhe: 0.75 ± 0.171
1.735MetGly: 1.735 ± 0.309
0.094MetHis: 0.094 ± 0.065
1.547MetIle: 1.547 ± 0.288
2.157MetLys: 2.157 ± 0.38
1.923MetLeu: 1.923 ± 0.292
0.563MetMet: 0.563 ± 0.148
1.547MetAsn: 1.547 ± 0.26
0.938MetPro: 0.938 ± 0.249
0.985MetGln: 0.985 ± 0.25
1.266MetArg: 1.266 ± 0.212
1.97MetSer: 1.97 ± 0.403
2.11MetThr: 2.11 ± 0.218
1.407MetVal: 1.407 ± 0.257
0.141MetTrp: 0.141 ± 0.098
0.657MetTyr: 0.657 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
5.346AsnAla: 5.346 ± 0.824
0.188AsnCys: 0.188 ± 0.097
2.954AsnAsp: 2.954 ± 0.308
2.954AsnGlu: 2.954 ± 0.415
1.876AsnPhe: 1.876 ± 0.351
6.471AsnGly: 6.471 ± 0.543
0.938AsnHis: 0.938 ± 0.239
2.814AsnIle: 2.814 ± 0.418
3.564AsnLys: 3.564 ± 0.448
3.517AsnLeu: 3.517 ± 0.385
0.844AsnMet: 0.844 ± 0.185
2.86AsnAsn: 2.86 ± 0.434
2.298AsnPro: 2.298 ± 0.341
2.954AsnGln: 2.954 ± 0.423
2.767AsnArg: 2.767 ± 0.314
4.22AsnSer: 4.22 ± 0.57
3.986AsnThr: 3.986 ± 0.38
3.798AsnVal: 3.798 ± 0.555
0.657AsnTrp: 0.657 ± 0.201
1.876AsnTyr: 1.876 ± 0.31
0.0AsnXaa: 0.0 ± 0.0
Pro
3.095ProAla: 3.095 ± 0.46
0.047ProCys: 0.047 ± 0.045
2.438ProAsp: 2.438 ± 0.4
2.532ProGlu: 2.532 ± 0.338
0.797ProPhe: 0.797 ± 0.181
1.876ProGly: 1.876 ± 0.339
0.469ProHis: 0.469 ± 0.166
1.782ProIle: 1.782 ± 0.246
1.923ProLys: 1.923 ± 0.296
2.157ProLeu: 2.157 ± 0.326
0.938ProMet: 0.938 ± 0.235
1.688ProAsn: 1.688 ± 0.253
0.563ProPro: 0.563 ± 0.147
0.891ProGln: 0.891 ± 0.206
1.125ProArg: 1.125 ± 0.22
1.641ProSer: 1.641 ± 0.266
2.251ProThr: 2.251 ± 0.395
2.532ProVal: 2.532 ± 0.323
0.094ProTrp: 0.094 ± 0.061
1.219ProTyr: 1.219 ± 0.304
0.0ProXaa: 0.0 ± 0.0
Gln
5.018GlnAla: 5.018 ± 0.68
0.094GlnCys: 0.094 ± 0.062
1.36GlnAsp: 1.36 ± 0.274
1.641GlnGlu: 1.641 ± 0.276
2.063GlnPhe: 2.063 ± 0.29
2.532GlnGly: 2.532 ± 0.366
0.844GlnHis: 0.844 ± 0.22
3.986GlnIle: 3.986 ± 0.373
2.251GlnLys: 2.251 ± 0.295
4.174GlnLeu: 4.174 ± 0.513
1.172GlnMet: 1.172 ± 0.236
1.641GlnAsn: 1.641 ± 0.269
1.735GlnPro: 1.735 ± 0.356
2.298GlnGln: 2.298 ± 0.281
1.735GlnArg: 1.735 ± 0.261
3.048GlnSer: 3.048 ± 0.36
3.751GlnThr: 3.751 ± 0.525
3.939GlnVal: 3.939 ± 0.379
0.75GlnTrp: 0.75 ± 0.203
2.251GlnTyr: 2.251 ± 0.348
0.0GlnXaa: 0.0 ± 0.0
Arg
3.048ArgAla: 3.048 ± 0.537
0.047ArgCys: 0.047 ± 0.04
2.532ArgAsp: 2.532 ± 0.398
2.016ArgGlu: 2.016 ± 0.351
1.219ArgPhe: 1.219 ± 0.193
1.547ArgGly: 1.547 ± 0.325
0.844ArgHis: 0.844 ± 0.191
2.251ArgIle: 2.251 ± 0.416
2.392ArgLys: 2.392 ± 0.394
2.298ArgLeu: 2.298 ± 0.29
1.032ArgMet: 1.032 ± 0.188
1.782ArgAsn: 1.782 ± 0.334
1.407ArgPro: 1.407 ± 0.274
1.36ArgGln: 1.36 ± 0.246
1.641ArgArg: 1.641 ± 0.362
1.876ArgSer: 1.876 ± 0.286
2.485ArgThr: 2.485 ± 0.319
2.673ArgVal: 2.673 ± 0.328
0.375ArgTrp: 0.375 ± 0.15
1.923ArgTyr: 1.923 ± 0.306
0.0ArgXaa: 0.0 ± 0.0
Ser
5.393SerAla: 5.393 ± 0.618
0.281SerCys: 0.281 ± 0.11
4.877SerAsp: 4.877 ± 0.6
3.001SerGlu: 3.001 ± 0.485
2.579SerPhe: 2.579 ± 0.43
4.596SerGly: 4.596 ± 0.765
1.079SerHis: 1.079 ± 0.178
4.642SerIle: 4.642 ± 0.494
5.674SerLys: 5.674 ± 0.978
6.471SerLeu: 6.471 ± 0.538
2.11SerMet: 2.11 ± 0.46
3.845SerAsn: 3.845 ± 0.443
1.641SerPro: 1.641 ± 0.296
4.033SerGln: 4.033 ± 0.565
1.876SerArg: 1.876 ± 0.277
5.393SerSer: 5.393 ± 0.517
5.533SerThr: 5.533 ± 0.655
4.127SerVal: 4.127 ± 0.41
1.125SerTrp: 1.125 ± 0.332
2.392SerTyr: 2.392 ± 0.325
0.0SerXaa: 0.0 ± 0.0
Thr
8.019ThrAla: 8.019 ± 0.763
0.281ThrCys: 0.281 ± 0.117
5.58ThrAsp: 5.58 ± 0.704
3.095ThrGlu: 3.095 ± 0.382
2.814ThrPhe: 2.814 ± 0.301
5.721ThrGly: 5.721 ± 0.641
0.891ThrHis: 0.891 ± 0.212
5.674ThrIle: 5.674 ± 0.562
5.346ThrLys: 5.346 ± 0.461
6.002ThrLeu: 6.002 ± 0.688
1.219ThrMet: 1.219 ± 0.206
4.267ThrAsn: 4.267 ± 0.39
2.485ThrPro: 2.485 ± 0.346
3.189ThrGln: 3.189 ± 0.475
1.923ThrArg: 1.923 ± 0.278
5.111ThrSer: 5.111 ± 0.626
5.205ThrThr: 5.205 ± 0.724
5.205ThrVal: 5.205 ± 0.744
0.891ThrTrp: 0.891 ± 0.223
2.767ThrTyr: 2.767 ± 0.456
0.0ThrXaa: 0.0 ± 0.0
Val
5.768ValAla: 5.768 ± 0.525
0.422ValCys: 0.422 ± 0.136
4.502ValAsp: 4.502 ± 0.568
3.189ValGlu: 3.189 ± 0.307
1.641ValPhe: 1.641 ± 0.226
4.783ValGly: 4.783 ± 0.556
1.313ValHis: 1.313 ± 0.184
4.549ValIle: 4.549 ± 0.427
5.252ValLys: 5.252 ± 0.497
5.299ValLeu: 5.299 ± 0.48
1.923ValMet: 1.923 ± 0.305
3.845ValAsn: 3.845 ± 0.786
1.501ValPro: 1.501 ± 0.264
2.438ValGln: 2.438 ± 0.34
2.063ValArg: 2.063 ± 0.29
3.658ValSer: 3.658 ± 0.347
5.862ValThr: 5.862 ± 0.477
3.751ValVal: 3.751 ± 0.519
0.469ValTrp: 0.469 ± 0.122
2.532ValTyr: 2.532 ± 0.368
0.0ValXaa: 0.0 ± 0.0
Trp
0.703TrpAla: 0.703 ± 0.21
0.094TrpCys: 0.094 ± 0.067
0.703TrpAsp: 0.703 ± 0.19
0.563TrpGlu: 0.563 ± 0.176
0.422TrpPhe: 0.422 ± 0.133
0.75TrpGly: 0.75 ± 0.196
0.141TrpHis: 0.141 ± 0.068
0.657TrpIle: 0.657 ± 0.175
1.079TrpLys: 1.079 ± 0.311
1.36TrpLeu: 1.36 ± 0.255
0.141TrpMet: 0.141 ± 0.079
0.985TrpAsn: 0.985 ± 0.303
0.094TrpPro: 0.094 ± 0.066
0.75TrpGln: 0.75 ± 0.241
0.516TrpArg: 0.516 ± 0.149
1.219TrpSer: 1.219 ± 0.365
0.891TrpThr: 0.891 ± 0.218
0.516TrpVal: 0.516 ± 0.131
0.234TrpTrp: 0.234 ± 0.105
0.422TrpTyr: 0.422 ± 0.18
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.814TyrAla: 2.814 ± 0.364
0.281TyrCys: 0.281 ± 0.117
2.251TyrAsp: 2.251 ± 0.3
1.735TyrGlu: 1.735 ± 0.327
1.688TyrPhe: 1.688 ± 0.346
3.423TyrGly: 3.423 ± 0.326
0.516TyrHis: 0.516 ± 0.183
1.735TyrIle: 1.735 ± 0.323
2.298TyrLys: 2.298 ± 0.341
3.705TyrLeu: 3.705 ± 0.475
0.657TyrMet: 0.657 ± 0.168
2.532TyrAsn: 2.532 ± 0.437
1.079TyrPro: 1.079 ± 0.221
2.11TyrGln: 2.11 ± 0.339
1.782TyrArg: 1.782 ± 0.299
2.016TyrSer: 2.016 ± 0.285
3.001TyrThr: 3.001 ± 0.457
2.063TyrVal: 2.063 ± 0.267
0.703TyrTrp: 0.703 ± 0.139
1.407TyrTyr: 1.407 ± 0.295
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (21326 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski