Anaerobacterium chartisolvens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Anaerobacterium

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A369APH6|A0A369APH6_9FIRM Phosphoesterase OS=Anaerobacterium chartisolvens OX=1297424 GN=DFR58_12642 PE=3 SV=1
MM1 pKa = 7.49SFLKK5 pKa = 10.6EE6 pKa = 3.62RR7 pKa = 11.84VSYY10 pKa = 10.96LKK12 pKa = 10.97GLAEE16 pKa = 4.36GMQLDD21 pKa = 3.79VSTNEE26 pKa = 3.72GKK28 pKa = 10.34LLKK31 pKa = 10.61AIIEE35 pKa = 4.38VMDD38 pKa = 4.55DD39 pKa = 2.97MALAVDD45 pKa = 5.31DD46 pKa = 4.14IEE48 pKa = 4.71EE49 pKa = 4.17VQEE52 pKa = 3.92QLSEE56 pKa = 4.1QVEE59 pKa = 4.73DD60 pKa = 4.08INDD63 pKa = 3.71DD64 pKa = 3.59LAEE67 pKa = 4.08VEE69 pKa = 4.42KK70 pKa = 11.03AVFDD74 pKa = 4.32EE75 pKa = 5.63DD76 pKa = 6.12DD77 pKa = 3.67EE78 pKa = 4.89HH79 pKa = 7.08CCCEE83 pKa = 5.1DD84 pKa = 4.09EE85 pKa = 5.78IEE87 pKa = 5.15CPYY90 pKa = 10.85CNEE93 pKa = 5.25KK94 pKa = 8.79ITLDD98 pKa = 3.69EE99 pKa = 4.67NLIADD104 pKa = 4.65DD105 pKa = 5.57GSTLKK110 pKa = 10.91CPSCGKK116 pKa = 10.04EE117 pKa = 4.65IEE119 pKa = 4.37IDD121 pKa = 3.37WDD123 pKa = 4.57CEE125 pKa = 4.2CEE127 pKa = 4.03DD128 pKa = 4.08CSEE131 pKa = 4.2HH132 pKa = 6.94EE133 pKa = 4.31KK134 pKa = 11.14

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A369BHK0|A0A369BHK0_9FIRM Uncharacterized protein OS=Anaerobacterium chartisolvens OX=1297424 GN=DFR58_101251 PE=4 SV=1
MM1 pKa = 7.78KK2 pKa = 10.11INARR6 pKa = 11.84ASQKK10 pKa = 9.53IAKK13 pKa = 9.32FLIWTAALLVIVVLFSIIIHH33 pKa = 6.12ILVKK37 pKa = 10.18GIPMLRR43 pKa = 11.84WQFLTDD49 pKa = 3.4IPRR52 pKa = 11.84DD53 pKa = 3.53MGRR56 pKa = 11.84SGGIASSIVGTLFVTAVAVIVATPFGIGTAIYY88 pKa = 7.98LTEE91 pKa = 3.9YY92 pKa = 8.99TRR94 pKa = 11.84EE95 pKa = 3.97GRR97 pKa = 11.84VTRR100 pKa = 11.84IIRR103 pKa = 11.84FSAEE107 pKa = 3.78SLAGIPSIVYY117 pKa = 10.07GLFGFIFFVIYY128 pKa = 10.64LGMGWSILSGGLTMAIMILPTIIRR152 pKa = 11.84TSEE155 pKa = 3.55EE156 pKa = 3.94AIRR159 pKa = 11.84TVPQLYY165 pKa = 9.82RR166 pKa = 11.84EE167 pKa = 4.34VGFSLGATKK176 pKa = 9.31WQAIIRR182 pKa = 11.84TVLPSAIPGIVNGVILSIGRR202 pKa = 11.84CVAEE206 pKa = 4.06TAAVILTAGSALRR219 pKa = 11.84MPASLLSPTRR229 pKa = 11.84TMAVHH234 pKa = 6.62FYY236 pKa = 10.04ILARR240 pKa = 11.84EE241 pKa = 4.8GISMDD246 pKa = 3.49NAYY249 pKa = 8.16GTAALLIILILLINVGFNMLVNRR272 pKa = 11.84FIAKK276 pKa = 9.44GRR278 pKa = 3.67

Molecular weight: 30.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4253

0

4253

1430626

27

8081

336.4

37.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.178 ± 0.043

1.35 ± 0.017

5.541 ± 0.028

6.908 ± 0.042

4.221 ± 0.034

7.378 ± 0.041

1.566 ± 0.016

8.37 ± 0.038

7.0 ± 0.039

8.806 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.81 ± 0.021

4.847 ± 0.029

3.34 ± 0.023

2.832 ± 0.018

4.488 ± 0.031

6.865 ± 0.042

4.914 ± 0.041

6.656 ± 0.03

0.933 ± 0.016

3.996 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski