Parabacteroides gordonii MS-1 = DSM 23371

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Tannerellaceae; Parabacteroides; Parabacteroides gordonii

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5316 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F5JA38|A0A0F5JA38_9BACT Uncharacterized protein OS=Parabacteroides gordonii MS-1 = DSM 23371 OX=1203610 GN=HMPREF1536_03481 PE=4 SV=1
MM1 pKa = 6.9EE2 pKa = 5.64AKK4 pKa = 10.07VLSEE8 pKa = 3.7AKK10 pKa = 10.47VYY12 pKa = 10.65VGTYY16 pKa = 9.99AKK18 pKa = 10.32YY19 pKa = 10.81NNGSLFGAWLDD30 pKa = 3.89LSDD33 pKa = 4.65YY34 pKa = 10.83SDD36 pKa = 3.73KK37 pKa = 11.66EE38 pKa = 4.09EE39 pKa = 4.45FYY41 pKa = 9.88EE42 pKa = 4.99ACRR45 pKa = 11.84EE46 pKa = 4.05LHH48 pKa = 6.63KK49 pKa = 11.13DD50 pKa = 3.63EE51 pKa = 5.18EE52 pKa = 4.44DD53 pKa = 3.48AEE55 pKa = 4.74YY56 pKa = 10.09MFQDD60 pKa = 3.69WEE62 pKa = 4.32NVPEE66 pKa = 4.11GLIGEE71 pKa = 4.39SWISEE76 pKa = 4.08NFFSLRR82 pKa = 11.84DD83 pKa = 3.67AVEE86 pKa = 4.73DD87 pKa = 4.54LDD89 pKa = 4.64DD90 pKa = 4.21TEE92 pKa = 4.6QEE94 pKa = 5.44AFFVWCNYY102 pKa = 9.34KK103 pKa = 10.33SHH105 pKa = 7.4DD106 pKa = 4.5LSEE109 pKa = 4.32EE110 pKa = 4.08DD111 pKa = 4.37ADD113 pKa = 4.86DD114 pKa = 5.36LIRR117 pKa = 11.84DD118 pKa = 3.54FRR120 pKa = 11.84DD121 pKa = 3.54EE122 pKa = 4.08YY123 pKa = 10.76RR124 pKa = 11.84GQYY127 pKa = 10.56DD128 pKa = 3.55DD129 pKa = 5.61EE130 pKa = 5.06EE131 pKa = 5.71DD132 pKa = 3.22FAYY135 pKa = 10.08EE136 pKa = 4.43IIEE139 pKa = 4.11EE140 pKa = 4.66CYY142 pKa = 10.66DD143 pKa = 3.52LPEE146 pKa = 4.02FAKK149 pKa = 10.18TYY151 pKa = 10.62FDD153 pKa = 3.75YY154 pKa = 11.44EE155 pKa = 4.0KK156 pKa = 10.43FARR159 pKa = 11.84DD160 pKa = 5.04LFMCDD165 pKa = 2.54YY166 pKa = 9.94WFDD169 pKa = 3.78DD170 pKa = 3.9GFVFRR175 pKa = 11.84AAA177 pKa = 3.87

Molecular weight: 21.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F5JAY9|A0A0F5JAY9_9BACT RNA polymerase sigma-70 factor OS=Parabacteroides gordonii MS-1 = DSM 23371 OX=1203610 GN=HMPREF1536_02479 PE=3 SV=1
MM1 pKa = 7.17EE2 pKa = 5.83RR3 pKa = 11.84KK4 pKa = 9.3RR5 pKa = 11.84NYY7 pKa = 8.95IGKK10 pKa = 8.93VRR12 pKa = 11.84DD13 pKa = 3.33WADD16 pKa = 3.0DD17 pKa = 3.52RR18 pKa = 11.84LRR20 pKa = 11.84RR21 pKa = 11.84MCGRR25 pKa = 11.84ITPGKK30 pKa = 8.59RR31 pKa = 11.84LAVILVMFFFLGGLSIYY48 pKa = 9.28ITVSSIYY55 pKa = 10.61NIGKK59 pKa = 9.2RR60 pKa = 11.84DD61 pKa = 3.47GRR63 pKa = 11.84RR64 pKa = 11.84LQIEE68 pKa = 5.25HH69 pKa = 6.75IRR71 pKa = 11.84QLPLHH76 pKa = 6.66GNDD79 pKa = 3.14TTKK82 pKa = 10.74SINPLNRR89 pKa = 11.84PQYY92 pKa = 10.4GRR94 pKa = 11.84STEE97 pKa = 4.05EE98 pKa = 3.56

Molecular weight: 11.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5316

0

5316

2028514

21

2493

381.6

42.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.974 ± 0.03

1.11 ± 0.011

5.683 ± 0.022

6.459 ± 0.03

4.539 ± 0.023

6.937 ± 0.03

1.685 ± 0.014

6.806 ± 0.032

6.562 ± 0.02

9.12 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.571 ± 0.014

5.307 ± 0.025

3.854 ± 0.019

3.424 ± 0.016

4.42 ± 0.022

6.349 ± 0.027

5.949 ± 0.031

6.452 ± 0.026

1.297 ± 0.012

4.503 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski