candidate division MSBL1 archaeon SCGC-AAA261F17

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133V6J2|A0A133V6J2_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA261F17 OX=1698274 GN=AKJ44_01525 PE=4 SV=1
MM1 pKa = 7.15YY2 pKa = 10.58LNGIGSGAEE11 pKa = 3.88NARR14 pKa = 11.84EE15 pKa = 4.06NPSGPSGPEE24 pKa = 3.34IEE26 pKa = 4.28NVRR29 pKa = 11.84EE30 pKa = 3.81NSTRR34 pKa = 11.84PSTPEE39 pKa = 3.51AEE41 pKa = 4.41NARR44 pKa = 11.84EE45 pKa = 4.0NSTGQPPASWWNEE58 pKa = 3.63HH59 pKa = 6.75DD60 pKa = 3.5LHH62 pKa = 7.21AIPGLEE68 pKa = 3.98NASVEE73 pKa = 4.26DD74 pKa = 4.14LPTAEE79 pKa = 4.15EE80 pKa = 4.39VEE82 pKa = 4.37NILFNWPVFEE92 pKa = 5.6NIWTGCWIDD101 pKa = 3.41WNGSYY106 pKa = 10.85QDD108 pKa = 3.71VLNILKK114 pKa = 10.29DD115 pKa = 3.85FNIEE119 pKa = 3.83YY120 pKa = 10.63HH121 pKa = 6.11FGEE124 pKa = 4.18NNKK127 pKa = 10.52LIINTPGIFYY137 pKa = 9.8KK138 pKa = 10.78KK139 pKa = 9.34PGEE142 pKa = 4.74PIPDD146 pKa = 3.11WMTLYY151 pKa = 11.04DD152 pKa = 3.65NSEE155 pKa = 3.88YY156 pKa = 10.78RR157 pKa = 11.84IWNYY161 pKa = 10.91SDD163 pKa = 3.43TLNSLYY169 pKa = 10.42CSRR172 pKa = 11.84YY173 pKa = 9.78LDD175 pKa = 3.35NLGIRR180 pKa = 11.84NGIAIIPVFTATPPLIEE197 pKa = 4.57AKK199 pKa = 10.41VVVPVRR205 pKa = 11.84KK206 pKa = 9.68GLAYY210 pKa = 9.61YY211 pKa = 10.04IPTAGEE217 pKa = 3.69RR218 pKa = 11.84AHH220 pKa = 7.06WAGIWYY226 pKa = 9.73ALNLKK231 pKa = 10.04PGGSNPYY238 pKa = 8.28YY239 pKa = 9.69TDD241 pKa = 4.1PEE243 pKa = 4.44SEE245 pKa = 4.04PLGDD249 pKa = 5.59IEE251 pKa = 4.22IHH253 pKa = 5.17WRR255 pKa = 11.84LGEE258 pKa = 4.11GEE260 pKa = 4.39YY261 pKa = 10.97VDD263 pKa = 4.43WLYY266 pKa = 10.71TPQDD270 pKa = 4.0GEE272 pKa = 4.73PVMTWSDD279 pKa = 3.11NGEE282 pKa = 4.04PVDD285 pKa = 3.58WW286 pKa = 5.27

Molecular weight: 32.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133V6Y7|A0A133V6Y7_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA261F17 OX=1698274 GN=AKJ44_01165 PE=4 SV=1
MM1 pKa = 7.66GSWQRR6 pKa = 11.84KK7 pKa = 7.66ALIALFYY14 pKa = 10.45PFTLLMIVAGFTAFVLLVFDD34 pKa = 5.04FSTFFAATVALCFFSFSATILYY56 pKa = 10.28LIFRR60 pKa = 11.84PVIKK64 pKa = 10.19LLNVRR69 pKa = 11.84RR70 pKa = 11.84IFLGLVVAADD80 pKa = 3.51VLAILSLGTLLLRR93 pKa = 11.84GIVV96 pKa = 3.26

Molecular weight: 10.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

559

0

559

134315

59

1253

240.3

26.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.903 ± 0.138

0.939 ± 0.047

5.583 ± 0.091

9.559 ± 0.145

3.533 ± 0.08

7.499 ± 0.109

1.846 ± 0.046

6.99 ± 0.086

7.421 ± 0.146

9.42 ± 0.121

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.178 ± 0.05

3.385 ± 0.074

4.209 ± 0.077

2.527 ± 0.061

5.871 ± 0.127

6.055 ± 0.08

5.043 ± 0.097

6.973 ± 0.083

1.167 ± 0.047

2.898 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski