Influenza A virus (A/Shanghai/02/2013(H7N9))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; H7N9 subtype

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4NQM4|R4NQM4_9INFA Matrix protein 2 OS=Influenza A virus (A/Shanghai/02/2013(H7N9)) OX=1332244 GN=M2 PE=3 SV=1
MM1 pKa = 7.38SLLTEE6 pKa = 4.41VEE8 pKa = 4.5TPTRR12 pKa = 11.84TGWEE16 pKa = 4.58CNCSGSSEE24 pKa = 4.19PLVVAANIIGILHH37 pKa = 7.21LILWILDD44 pKa = 3.39RR45 pKa = 11.84LFFKK49 pKa = 10.77CIYY52 pKa = 10.14RR53 pKa = 11.84RR54 pKa = 11.84FKK56 pKa = 10.97YY57 pKa = 10.01GLKK60 pKa = 10.11RR61 pKa = 11.84GPSTEE66 pKa = 3.92GMPEE70 pKa = 3.98SMRR73 pKa = 11.84EE74 pKa = 3.94EE75 pKa = 4.07YY76 pKa = 10.41RR77 pKa = 11.84QEE79 pKa = 3.67QQNAVDD85 pKa = 3.55VDD87 pKa = 3.97DD88 pKa = 4.06GHH90 pKa = 6.57FVNIEE95 pKa = 3.82LKK97 pKa = 10.68

Molecular weight: 11.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4NFR6|R4NFR6_9INFA Neuraminidase OS=Influenza A virus (A/Shanghai/02/2013(H7N9)) OX=1332244 GN=NA PE=1 SV=1
MM1 pKa = 7.21EE2 pKa = 4.72QEE4 pKa = 4.77QDD6 pKa = 3.96TPWTQSTEE14 pKa = 4.23HH15 pKa = 6.9INTQKK20 pKa = 10.74KK21 pKa = 9.91EE22 pKa = 4.06SGQRR26 pKa = 11.84TQRR29 pKa = 11.84LEE31 pKa = 4.12HH32 pKa = 6.79PNSIQLMDD40 pKa = 3.93HH41 pKa = 5.9YY42 pKa = 11.17LRR44 pKa = 11.84TTSRR48 pKa = 11.84VGMHH52 pKa = 5.87KK53 pKa = 10.36RR54 pKa = 11.84IVYY57 pKa = 7.32WKK59 pKa = 9.84QWLSLKK65 pKa = 10.64NLTQGSLKK73 pKa = 9.9TRR75 pKa = 11.84VSKK78 pKa = 10.39RR79 pKa = 11.84WKK81 pKa = 10.3LFSKK85 pKa = 9.91QEE87 pKa = 4.12WINN90 pKa = 3.33

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

4784

90

759

398.7

45.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.041 ± 0.388

1.86 ± 0.365

4.62 ± 0.224

7.525 ± 0.67

3.909 ± 0.333

6.417 ± 0.46

1.693 ± 0.136

6.501 ± 0.271

5.769 ± 0.54

7.839 ± 0.425

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.888 ± 0.387

5.456 ± 0.453

3.804 ± 0.233

4.055 ± 0.389

7.128 ± 0.566

7.274 ± 0.331

6.605 ± 0.338

5.372 ± 0.547

1.589 ± 0.19

2.655 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski