Freshwater phage uvFW-CGR-AMDFOS-S50-C341

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0XUQ9|A0A1B0XUQ9_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMDFOS-S50-C341 OX=1838158 GN=uvFWCGRAMDFOSS50C341_015 PE=4 SV=1
MM1 pKa = 7.07ITATCINPDD10 pKa = 3.87CAYY13 pKa = 9.87KK14 pKa = 10.63GYY16 pKa = 9.66PVDD19 pKa = 4.1IDD21 pKa = 3.97GSPDD25 pKa = 3.22PVMCGVCGQEE35 pKa = 6.07DD36 pKa = 5.04EE37 pKa = 4.73LTDD40 pKa = 3.83PRR42 pKa = 11.84PDD44 pKa = 3.94PGTTPTEE51 pKa = 4.03PP52 pKa = 3.38

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0XUM7|A0A1B0XUM7_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMDFOS-S50-C341 OX=1838158 GN=uvFWCGRAMDFOSS50C341_010 PE=4 SV=1
MM1 pKa = 6.88TTDD4 pKa = 3.64LTIQVDD10 pKa = 4.47GVKK13 pKa = 10.29EE14 pKa = 3.76AVKK17 pKa = 10.59YY18 pKa = 10.56LNQVEE23 pKa = 4.21PGFRR27 pKa = 11.84KK28 pKa = 10.16AYY30 pKa = 7.31VANMKK35 pKa = 9.82EE36 pKa = 4.32VARR39 pKa = 11.84PMTEE43 pKa = 3.75AMKK46 pKa = 10.57SQYY49 pKa = 10.83DD50 pKa = 3.73DD51 pKa = 3.56NRR53 pKa = 11.84FPSGTKK59 pKa = 10.25RR60 pKa = 11.84NWAPEE65 pKa = 3.75GRR67 pKa = 11.84QVFPLSASNAVRR79 pKa = 11.84GVSLRR84 pKa = 11.84VNNKK88 pKa = 9.24KK89 pKa = 10.35VGAAFSVMQKK99 pKa = 10.2NPAAAIFDD107 pKa = 3.51IAGRR111 pKa = 11.84ANVNPLGAAFSTKK124 pKa = 10.25FGRR127 pKa = 11.84SASRR131 pKa = 11.84VIWPVFEE138 pKa = 4.23TKK140 pKa = 9.9IVQISEE146 pKa = 4.54EE147 pKa = 3.92IQKK150 pKa = 9.75VVNGVMAEE158 pKa = 4.05VNKK161 pKa = 10.36NMKK164 pKa = 10.15VII166 pKa = 3.86

Molecular weight: 18.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

3902

52

517

177.4

19.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.456 ± 0.968

1.051 ± 0.266

5.1 ± 0.325

4.895 ± 0.517

4.177 ± 0.306

7.304 ± 0.417

0.999 ± 0.327

5.407 ± 0.376

4.152 ± 0.526

7.817 ± 0.549

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.998 ± 0.278

4.69 ± 0.265

5.074 ± 0.442

4.28 ± 0.366

3.947 ± 0.428

5.997 ± 0.542

8.739 ± 0.712

7.022 ± 0.549

1.486 ± 0.179

3.409 ± 0.484
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski