Amino acid dipepetide frequency for Microbacterium phage Koji

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.007AlaAla: 11.007 ± 1.275
0.703AlaCys: 0.703 ± 0.228
6.245AlaAsp: 6.245 ± 0.679
5.933AlaGlu: 5.933 ± 0.726
3.123AlaPhe: 3.123 ± 0.593
8.431AlaGly: 8.431 ± 1.019
1.952AlaHis: 1.952 ± 0.367
4.294AlaIle: 4.294 ± 0.823
6.011AlaLys: 6.011 ± 1.063
11.085AlaLeu: 11.085 ± 1.455
2.42AlaMet: 2.42 ± 0.391
3.044AlaAsn: 3.044 ± 0.421
3.981AlaPro: 3.981 ± 0.596
3.591AlaGln: 3.591 ± 0.444
6.089AlaArg: 6.089 ± 0.795
4.762AlaSer: 4.762 ± 0.631
6.401AlaThr: 6.401 ± 0.642
6.948AlaVal: 6.948 ± 0.852
1.717AlaTrp: 1.717 ± 0.479
3.044AlaTyr: 3.044 ± 0.616
0.0AlaXaa: 0.0 ± 0.0
Cys
0.625CysAla: 0.625 ± 0.201
0.0CysCys: 0.0 ± 0.0
0.468CysAsp: 0.468 ± 0.186
0.546CysGlu: 0.546 ± 0.201
0.156CysPhe: 0.156 ± 0.1
0.625CysGly: 0.625 ± 0.221
0.234CysHis: 0.234 ± 0.126
0.234CysIle: 0.234 ± 0.153
0.312CysLys: 0.312 ± 0.154
0.156CysLeu: 0.156 ± 0.111
0.0CysMet: 0.0 ± 0.0
0.078CysAsn: 0.078 ± 0.072
0.546CysPro: 0.546 ± 0.247
0.312CysGln: 0.312 ± 0.164
0.625CysArg: 0.625 ± 0.263
0.468CysSer: 0.468 ± 0.204
0.625CysThr: 0.625 ± 0.213
0.39CysVal: 0.39 ± 0.155
0.234CysTrp: 0.234 ± 0.111
0.234CysTyr: 0.234 ± 0.126
0.0CysXaa: 0.0 ± 0.0
Asp
4.684AspAla: 4.684 ± 0.695
0.546AspCys: 0.546 ± 0.187
5.23AspAsp: 5.23 ± 1.107
4.137AspGlu: 4.137 ± 1.014
3.044AspPhe: 3.044 ± 0.461
4.294AspGly: 4.294 ± 0.749
1.561AspHis: 1.561 ± 0.396
3.123AspIle: 3.123 ± 0.417
2.732AspLys: 2.732 ± 0.479
5.464AspLeu: 5.464 ± 0.524
1.561AspMet: 1.561 ± 0.304
1.717AspAsn: 1.717 ± 0.352
5.074AspPro: 5.074 ± 0.663
2.264AspGln: 2.264 ± 0.396
3.123AspArg: 3.123 ± 0.554
3.669AspSer: 3.669 ± 0.466
3.357AspThr: 3.357 ± 0.642
4.684AspVal: 4.684 ± 0.555
1.874AspTrp: 1.874 ± 0.427
2.654AspTyr: 2.654 ± 0.523
0.0AspXaa: 0.0 ± 0.0
Glu
8.119GluAla: 8.119 ± 0.763
0.468GluCys: 0.468 ± 0.209
5.386GluAsp: 5.386 ± 1.24
5.621GluGlu: 5.621 ± 1.133
1.327GluPhe: 1.327 ± 0.349
3.825GluGly: 3.825 ± 0.578
0.937GluHis: 0.937 ± 0.281
1.795GluIle: 1.795 ± 0.381
1.639GluLys: 1.639 ± 0.384
5.855GluLeu: 5.855 ± 0.758
1.405GluMet: 1.405 ± 0.328
2.342GluAsn: 2.342 ± 0.375
1.561GluPro: 1.561 ± 0.438
2.576GluGln: 2.576 ± 0.386
3.903GluArg: 3.903 ± 0.639
2.342GluSer: 2.342 ± 0.427
4.528GluThr: 4.528 ± 0.534
4.45GluVal: 4.45 ± 0.748
1.639GluTrp: 1.639 ± 0.408
1.952GluTyr: 1.952 ± 0.444
0.0GluXaa: 0.0 ± 0.0
Phe
2.576PheAla: 2.576 ± 0.392
0.312PheCys: 0.312 ± 0.136
1.874PheAsp: 1.874 ± 0.419
1.483PheGlu: 1.483 ± 0.339
0.859PhePhe: 0.859 ± 0.284
3.123PheGly: 3.123 ± 0.441
0.546PheHis: 0.546 ± 0.206
1.015PheIle: 1.015 ± 0.284
1.952PheLys: 1.952 ± 0.404
2.42PheLeu: 2.42 ± 0.378
0.781PheMet: 0.781 ± 0.322
1.483PheAsn: 1.483 ± 0.283
1.405PhePro: 1.405 ± 0.279
1.015PheGln: 1.015 ± 0.305
2.108PheArg: 2.108 ± 0.331
2.42PheSer: 2.42 ± 0.419
2.498PheThr: 2.498 ± 0.463
1.483PheVal: 1.483 ± 0.361
0.39PheTrp: 0.39 ± 0.223
0.703PheTyr: 0.703 ± 0.213
0.0PheXaa: 0.0 ± 0.0
Gly
6.87GlyAla: 6.87 ± 0.82
0.703GlyCys: 0.703 ± 0.226
5.386GlyAsp: 5.386 ± 0.565
4.918GlyGlu: 4.918 ± 0.631
2.732GlyPhe: 2.732 ± 0.486
6.245GlyGly: 6.245 ± 1.096
1.249GlyHis: 1.249 ± 0.319
4.059GlyIle: 4.059 ± 0.915
4.372GlyLys: 4.372 ± 0.737
6.323GlyLeu: 6.323 ± 0.894
1.874GlyMet: 1.874 ± 0.32
2.966GlyAsn: 2.966 ± 0.504
2.654GlyPro: 2.654 ± 0.392
4.528GlyGln: 4.528 ± 0.691
4.294GlyArg: 4.294 ± 0.543
5.855GlySer: 5.855 ± 0.798
7.26GlyThr: 7.26 ± 0.858
6.167GlyVal: 6.167 ± 0.902
1.405GlyTrp: 1.405 ± 0.312
2.42GlyTyr: 2.42 ± 0.409
0.0GlyXaa: 0.0 ± 0.0
His
1.483HisAla: 1.483 ± 0.346
0.0HisCys: 0.0 ± 0.0
0.937HisAsp: 0.937 ± 0.306
1.795HisGlu: 1.795 ± 0.378
0.468HisPhe: 0.468 ± 0.199
1.952HisGly: 1.952 ± 0.43
0.468HisHis: 0.468 ± 0.24
1.015HisIle: 1.015 ± 0.342
1.327HisLys: 1.327 ± 0.345
1.327HisLeu: 1.327 ± 0.389
0.234HisMet: 0.234 ± 0.178
0.546HisAsn: 0.546 ± 0.234
1.015HisPro: 1.015 ± 0.258
0.703HisGln: 0.703 ± 0.214
0.625HisArg: 0.625 ± 0.188
0.39HisSer: 0.39 ± 0.184
1.015HisThr: 1.015 ± 0.286
1.483HisVal: 1.483 ± 0.352
0.546HisTrp: 0.546 ± 0.184
1.093HisTyr: 1.093 ± 0.259
0.0HisXaa: 0.0 ± 0.0
Ile
4.45IleAla: 4.45 ± 0.563
0.39IleCys: 0.39 ± 0.173
3.357IleAsp: 3.357 ± 0.539
2.966IleGlu: 2.966 ± 0.505
0.703IlePhe: 0.703 ± 0.272
3.357IleGly: 3.357 ± 0.876
0.937IleHis: 0.937 ± 0.299
2.498IleIle: 2.498 ± 0.795
1.874IleLys: 1.874 ± 0.427
3.123IleLeu: 3.123 ± 0.458
1.171IleMet: 1.171 ± 0.243
2.186IleAsn: 2.186 ± 0.549
2.264IlePro: 2.264 ± 0.793
2.264IleGln: 2.264 ± 0.685
2.654IleArg: 2.654 ± 0.482
2.264IleSer: 2.264 ± 0.508
4.294IleThr: 4.294 ± 0.857
3.435IleVal: 3.435 ± 0.695
0.546IleTrp: 0.546 ± 0.227
1.093IleTyr: 1.093 ± 0.302
0.0IleXaa: 0.0 ± 0.0
Lys
5.777LysAla: 5.777 ± 1.051
0.156LysCys: 0.156 ± 0.107
2.342LysAsp: 2.342 ± 0.432
3.044LysGlu: 3.044 ± 0.562
1.405LysPhe: 1.405 ± 0.265
4.45LysGly: 4.45 ± 0.777
0.937LysHis: 0.937 ± 0.257
2.03LysIle: 2.03 ± 0.389
2.498LysLys: 2.498 ± 0.583
4.528LysLeu: 4.528 ± 0.686
1.483LysMet: 1.483 ± 0.31
1.483LysAsn: 1.483 ± 0.376
3.123LysPro: 3.123 ± 0.637
1.093LysGln: 1.093 ± 0.253
3.123LysArg: 3.123 ± 0.527
1.717LysSer: 1.717 ± 0.379
3.044LysThr: 3.044 ± 0.552
3.591LysVal: 3.591 ± 0.558
0.859LysTrp: 0.859 ± 0.234
0.546LysTyr: 0.546 ± 0.22
0.0LysXaa: 0.0 ± 0.0
Leu
9.368LeuAla: 9.368 ± 0.968
0.625LeuCys: 0.625 ± 0.287
4.996LeuAsp: 4.996 ± 0.615
4.762LeuGlu: 4.762 ± 0.531
1.795LeuPhe: 1.795 ± 0.282
8.431LeuGly: 8.431 ± 0.931
1.561LeuHis: 1.561 ± 0.366
3.981LeuIle: 3.981 ± 1.038
4.372LeuLys: 4.372 ± 0.688
7.728LeuLeu: 7.728 ± 0.949
1.874LeuMet: 1.874 ± 0.367
2.966LeuAsn: 2.966 ± 0.52
5.074LeuPro: 5.074 ± 0.889
2.732LeuGln: 2.732 ± 0.43
4.684LeuArg: 4.684 ± 0.648
4.918LeuSer: 4.918 ± 0.549
4.918LeuThr: 4.918 ± 0.59
6.948LeuVal: 6.948 ± 0.941
0.859LeuTrp: 0.859 ± 0.216
2.108LeuTyr: 2.108 ± 0.388
0.0LeuXaa: 0.0 ± 0.0
Met
3.591MetAla: 3.591 ± 0.444
0.156MetCys: 0.156 ± 0.117
1.327MetAsp: 1.327 ± 0.334
0.859MetGlu: 0.859 ± 0.336
0.703MetPhe: 0.703 ± 0.206
1.405MetGly: 1.405 ± 0.345
0.312MetHis: 0.312 ± 0.148
1.093MetIle: 1.093 ± 0.27
1.171MetLys: 1.171 ± 0.288
2.03MetLeu: 2.03 ± 0.384
0.39MetMet: 0.39 ± 0.191
0.937MetAsn: 0.937 ± 0.229
0.781MetPro: 0.781 ± 0.266
1.405MetGln: 1.405 ± 0.295
0.937MetArg: 0.937 ± 0.24
2.264MetSer: 2.264 ± 0.436
2.186MetThr: 2.186 ± 0.376
1.093MetVal: 1.093 ± 0.28
0.156MetTrp: 0.156 ± 0.106
0.156MetTyr: 0.156 ± 0.109
0.0MetXaa: 0.0 ± 0.0
Asn
2.732AsnAla: 2.732 ± 0.605
0.312AsnCys: 0.312 ± 0.179
2.186AsnAsp: 2.186 ± 0.463
2.108AsnGlu: 2.108 ± 0.424
0.703AsnPhe: 0.703 ± 0.224
3.279AsnGly: 3.279 ± 0.482
0.39AsnHis: 0.39 ± 0.163
1.483AsnIle: 1.483 ± 0.571
1.639AsnLys: 1.639 ± 0.347
3.044AsnLeu: 3.044 ± 0.434
0.781AsnMet: 0.781 ± 0.285
1.015AsnAsn: 1.015 ± 0.29
2.186AsnPro: 2.186 ± 0.391
1.171AsnGln: 1.171 ± 0.233
1.874AsnArg: 1.874 ± 0.398
1.952AsnSer: 1.952 ± 0.479
2.342AsnThr: 2.342 ± 0.474
2.42AsnVal: 2.42 ± 0.465
1.015AsnTrp: 1.015 ± 0.299
0.937AsnTyr: 0.937 ± 0.302
0.0AsnXaa: 0.0 ± 0.0
Pro
4.684ProAla: 4.684 ± 0.735
0.156ProCys: 0.156 ± 0.179
3.435ProAsp: 3.435 ± 0.53
3.123ProGlu: 3.123 ± 0.547
1.249ProPhe: 1.249 ± 0.336
4.215ProGly: 4.215 ± 0.642
0.937ProHis: 0.937 ± 0.252
1.639ProIle: 1.639 ± 0.295
2.264ProLys: 2.264 ± 0.387
3.201ProLeu: 3.201 ± 0.457
0.937ProMet: 0.937 ± 0.23
1.717ProAsn: 1.717 ± 0.366
1.483ProPro: 1.483 ± 0.32
2.108ProGln: 2.108 ± 0.386
2.576ProArg: 2.576 ± 0.668
3.669ProSer: 3.669 ± 0.632
3.825ProThr: 3.825 ± 0.773
4.215ProVal: 4.215 ± 0.554
0.859ProTrp: 0.859 ± 0.259
1.249ProTyr: 1.249 ± 0.297
0.0ProXaa: 0.0 ± 0.0
Gln
5.464GlnAla: 5.464 ± 0.735
0.078GlnCys: 0.078 ± 0.071
2.186GlnAsp: 2.186 ± 0.415
2.732GlnGlu: 2.732 ± 0.445
1.015GlnPhe: 1.015 ± 0.295
3.044GlnGly: 3.044 ± 0.517
0.859GlnHis: 0.859 ± 0.223
1.874GlnIle: 1.874 ± 0.386
1.171GlnLys: 1.171 ± 0.305
3.201GlnLeu: 3.201 ± 0.567
0.781GlnMet: 0.781 ± 0.205
1.717GlnAsn: 1.717 ± 0.432
1.717GlnPro: 1.717 ± 0.34
2.264GlnGln: 2.264 ± 0.392
3.591GlnArg: 3.591 ± 0.53
2.108GlnSer: 2.108 ± 0.357
2.342GlnThr: 2.342 ± 0.478
3.513GlnVal: 3.513 ± 0.587
0.937GlnTrp: 0.937 ± 0.339
1.249GlnTyr: 1.249 ± 0.276
0.0GlnXaa: 0.0 ± 0.0
Arg
5.152ArgAla: 5.152 ± 0.826
0.468ArgCys: 0.468 ± 0.203
3.591ArgAsp: 3.591 ± 0.496
3.123ArgGlu: 3.123 ± 0.452
2.108ArgPhe: 2.108 ± 0.367
4.294ArgGly: 4.294 ± 0.627
0.781ArgHis: 0.781 ± 0.307
3.357ArgIle: 3.357 ± 0.489
3.435ArgLys: 3.435 ± 0.615
6.011ArgLeu: 6.011 ± 0.871
1.639ArgMet: 1.639 ± 0.333
2.03ArgAsn: 2.03 ± 0.466
2.576ArgPro: 2.576 ± 0.505
2.108ArgGln: 2.108 ± 0.408
3.825ArgArg: 3.825 ± 0.589
3.279ArgSer: 3.279 ± 0.541
2.81ArgThr: 2.81 ± 0.702
5.152ArgVal: 5.152 ± 0.748
1.405ArgTrp: 1.405 ± 0.434
1.483ArgTyr: 1.483 ± 0.413
0.0ArgXaa: 0.0 ± 0.0
Ser
4.918SerAla: 4.918 ± 0.755
0.312SerCys: 0.312 ± 0.168
3.903SerAsp: 3.903 ± 0.573
2.966SerGlu: 2.966 ± 0.516
2.498SerPhe: 2.498 ± 0.425
5.074SerGly: 5.074 ± 0.785
1.249SerHis: 1.249 ± 0.384
2.576SerIle: 2.576 ± 0.511
3.123SerLys: 3.123 ± 0.471
3.903SerLeu: 3.903 ± 0.597
2.108SerMet: 2.108 ± 0.397
1.717SerAsn: 1.717 ± 0.321
2.264SerPro: 2.264 ± 0.395
3.044SerGln: 3.044 ± 0.648
3.279SerArg: 3.279 ± 0.458
4.059SerSer: 4.059 ± 0.581
4.294SerThr: 4.294 ± 0.64
3.981SerVal: 3.981 ± 0.568
1.327SerTrp: 1.327 ± 0.303
1.952SerTyr: 1.952 ± 0.38
0.0SerXaa: 0.0 ± 0.0
Thr
7.104ThrAla: 7.104 ± 1.095
0.39ThrCys: 0.39 ± 0.176
3.903ThrAsp: 3.903 ± 0.658
3.044ThrGlu: 3.044 ± 0.668
3.044ThrPhe: 3.044 ± 0.483
6.401ThrGly: 6.401 ± 0.543
1.093ThrHis: 1.093 ± 0.313
2.342ThrIle: 2.342 ± 0.409
3.279ThrLys: 3.279 ± 0.542
5.308ThrLeu: 5.308 ± 0.877
1.171ThrMet: 1.171 ± 0.327
1.405ThrAsn: 1.405 ± 0.285
4.215ThrPro: 4.215 ± 0.62
2.42ThrGln: 2.42 ± 0.473
3.903ThrArg: 3.903 ± 0.573
4.918ThrSer: 4.918 ± 0.788
3.669ThrThr: 3.669 ± 0.592
6.557ThrVal: 6.557 ± 0.724
1.795ThrTrp: 1.795 ± 0.397
2.186ThrTyr: 2.186 ± 0.387
0.0ThrXaa: 0.0 ± 0.0
Val
7.963ValAla: 7.963 ± 0.73
0.312ValCys: 0.312 ± 0.134
4.918ValAsp: 4.918 ± 0.642
4.606ValGlu: 4.606 ± 0.706
1.717ValPhe: 1.717 ± 0.487
4.84ValGly: 4.84 ± 0.82
1.327ValHis: 1.327 ± 0.383
4.528ValIle: 4.528 ± 0.701
3.044ValLys: 3.044 ± 0.505
6.245ValLeu: 6.245 ± 0.816
1.483ValMet: 1.483 ± 0.415
2.42ValAsn: 2.42 ± 0.376
3.747ValPro: 3.747 ± 0.549
3.903ValGln: 3.903 ± 0.615
4.215ValArg: 4.215 ± 0.615
4.528ValSer: 4.528 ± 0.6
5.777ValThr: 5.777 ± 0.66
5.543ValVal: 5.543 ± 0.94
1.561ValTrp: 1.561 ± 0.361
2.732ValTyr: 2.732 ± 0.532
0.0ValXaa: 0.0 ± 0.0
Trp
1.483TrpAla: 1.483 ± 0.299
0.156TrpCys: 0.156 ± 0.121
1.171TrpAsp: 1.171 ± 0.304
1.171TrpGlu: 1.171 ± 0.308
0.937TrpPhe: 0.937 ± 0.289
1.717TrpGly: 1.717 ± 0.326
0.703TrpHis: 0.703 ± 0.23
1.405TrpIle: 1.405 ± 0.402
0.781TrpLys: 0.781 ± 0.197
1.874TrpLeu: 1.874 ± 0.387
0.156TrpMet: 0.156 ± 0.112
0.703TrpAsn: 0.703 ± 0.215
0.859TrpPro: 0.859 ± 0.289
1.015TrpGln: 1.015 ± 0.3
1.171TrpArg: 1.171 ± 0.303
0.781TrpSer: 0.781 ± 0.277
1.483TrpThr: 1.483 ± 0.343
1.405TrpVal: 1.405 ± 0.356
0.781TrpTrp: 0.781 ± 0.272
0.859TrpTyr: 0.859 ± 0.278
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.732TyrAla: 2.732 ± 0.416
0.625TyrCys: 0.625 ± 0.238
1.952TyrAsp: 1.952 ± 0.422
2.732TyrGlu: 2.732 ± 0.692
0.859TyrPhe: 0.859 ± 0.227
3.279TyrGly: 3.279 ± 0.422
0.39TyrHis: 0.39 ± 0.218
1.483TyrIle: 1.483 ± 0.275
0.234TyrLys: 0.234 ± 0.121
1.874TyrLeu: 1.874 ± 0.389
0.625TyrMet: 0.625 ± 0.285
1.093TyrAsn: 1.093 ± 0.369
1.015TyrPro: 1.015 ± 0.297
1.327TyrGln: 1.327 ± 0.306
2.03TyrArg: 2.03 ± 0.48
2.186TyrSer: 2.186 ± 0.5
1.483TyrThr: 1.483 ± 0.403
2.03TyrVal: 2.03 ± 0.555
0.703TyrTrp: 0.703 ± 0.221
1.093TyrTyr: 1.093 ± 0.345
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (12811 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski