Nocardioides sp. HDW12A

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3471 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G7YL10|A0A6G7YL10_9ACTN NUDIX domain-containing protein OS=Nocardioides sp. HDW12A OX=2714938 GN=G7071_13140 PE=4 SV=1
MM1 pKa = 7.66TGTLLEE7 pKa = 4.78PPFEE11 pKa = 4.67VNGAALVDD19 pKa = 4.98GSQSPYY25 pKa = 11.33SLVEE29 pKa = 4.16DD30 pKa = 3.96TDD32 pKa = 4.62DD33 pKa = 4.25PLTLVFFGYY42 pKa = 6.93THH44 pKa = 7.09CPDD47 pKa = 2.63ICGTVMSTMASAMTRR62 pKa = 11.84LSEE65 pKa = 4.22EE66 pKa = 4.1DD67 pKa = 3.4RR68 pKa = 11.84SDD70 pKa = 3.42VGVVFVTTDD79 pKa = 3.42PQRR82 pKa = 11.84DD83 pKa = 3.89TPAVSADD90 pKa = 3.27YY91 pKa = 8.33ATNFDD96 pKa = 3.92PGFVGVSGDD105 pKa = 3.88LDD107 pKa = 4.22TISEE111 pKa = 4.32VARR114 pKa = 11.84PLGIAVEE121 pKa = 4.33QGDD124 pKa = 4.21KK125 pKa = 10.92LPSGGYY131 pKa = 10.06DD132 pKa = 3.24VTHH135 pKa = 6.5GTQIIAVDD143 pKa = 4.05ADD145 pKa = 3.98DD146 pKa = 4.73QGIAYY151 pKa = 8.74WSEE154 pKa = 4.0AVSSAQLAADD164 pKa = 3.66IEE166 pKa = 4.54HH167 pKa = 6.99LLSEE171 pKa = 4.73GAA173 pKa = 3.94

Molecular weight: 18.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G7YKF7|A0A6G7YKF7_9ACTN NUDIX domain-containing protein OS=Nocardioides sp. HDW12A OX=2714938 GN=G7071_03645 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3471

0

3471

1077956

33

1530

310.6

33.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.822 ± 0.057

0.77 ± 0.013

6.553 ± 0.032

5.788 ± 0.035

2.882 ± 0.023

9.008 ± 0.031

2.25 ± 0.022

3.661 ± 0.028

2.163 ± 0.031

10.159 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.959 ± 0.017

1.825 ± 0.021

5.496 ± 0.034

2.812 ± 0.022

7.47 ± 0.048

5.624 ± 0.028

6.097 ± 0.035

9.309 ± 0.035

1.521 ± 0.02

1.83 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski