Apis mellifera associated microvirus 52

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5Y3|A0A3Q8U5Y3_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 52 OX=2494783 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.31KK3 pKa = 10.08KK4 pKa = 10.54NSDD7 pKa = 3.14MRR9 pKa = 11.84HH10 pKa = 4.99YY11 pKa = 10.51EE12 pKa = 3.95AVDD15 pKa = 3.92SNPHH19 pKa = 5.01AMAVKK24 pKa = 9.78IQRR27 pKa = 11.84PPTLAEE33 pKa = 3.82QIQAYY38 pKa = 8.55MGQHH42 pKa = 5.22NRR44 pKa = 11.84RR45 pKa = 11.84LEE47 pKa = 3.91QLGVEE52 pKa = 4.38TAEE55 pKa = 4.14EE56 pKa = 4.04ADD58 pKa = 4.05DD59 pKa = 5.22FDD61 pKa = 6.21VEE63 pKa = 5.72DD64 pKa = 4.3EE65 pKa = 4.7DD66 pKa = 5.76APEE69 pKa = 4.38SPHH72 pKa = 6.23EE73 pKa = 4.09LVYY76 pKa = 11.06DD77 pKa = 3.85PLLNRR82 pKa = 11.84EE83 pKa = 4.07LPRR86 pKa = 11.84YY87 pKa = 9.33QKK89 pKa = 11.12VLIDD93 pKa = 3.35QDD95 pKa = 3.39RR96 pKa = 11.84AKK98 pKa = 10.59FDD100 pKa = 3.34EE101 pKa = 4.02QLADD105 pKa = 4.07KK106 pKa = 10.69IRR108 pKa = 11.84ADD110 pKa = 3.8RR111 pKa = 11.84LAAEE115 pKa = 4.42AARR118 pKa = 11.84RR119 pKa = 11.84ARR121 pKa = 11.84AHH123 pKa = 6.42LEE125 pKa = 4.3SNGGNAGRR133 pKa = 11.84QIEE136 pKa = 4.64TTTEE140 pKa = 4.04EE141 pKa = 4.2PSEE144 pKa = 4.21DD145 pKa = 3.35

Molecular weight: 16.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UU80|A0A3S8UU80_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 52 OX=2494783 PE=3 SV=1
MM1 pKa = 7.35MGRR4 pKa = 11.84SRR6 pKa = 11.84NRR8 pKa = 11.84TRR10 pKa = 11.84ATVSNRR16 pKa = 11.84RR17 pKa = 11.84LRR19 pKa = 11.84STTTTTHH26 pKa = 6.55FSSTPAIDD34 pKa = 4.16LNRR37 pKa = 11.84LAALLSPPSLQNNYY51 pKa = 6.88STRR54 pKa = 11.84RR55 pKa = 11.84TARR58 pKa = 11.84VYY60 pKa = 10.72APPRR64 pKa = 11.84TTTPSQPPRR73 pKa = 11.84AQQRR77 pKa = 11.84RR78 pKa = 11.84LRR80 pKa = 11.84SNTSLSTGVWHH91 pKa = 6.2QVGSPVRR98 pKa = 11.84KK99 pKa = 9.31PSDD102 pKa = 3.12EE103 pKa = 4.43HH104 pKa = 8.41KK105 pKa = 10.39PDD107 pKa = 3.39EE108 pKa = 4.44ALVCVSRR115 pKa = 11.84SEE117 pKa = 4.06RR118 pKa = 11.84RR119 pKa = 11.84EE120 pKa = 3.72VLHH123 pKa = 6.98ALRR126 pKa = 11.84KK127 pKa = 7.68TGKK130 pKa = 8.26TGQKK134 pKa = 9.52SPKK137 pKa = 9.07FSRR140 pKa = 11.84ASKK143 pKa = 9.34IHH145 pKa = 6.16CKK147 pKa = 10.1KK148 pKa = 10.77

Molecular weight: 16.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1359

145

495

271.8

30.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.713 ± 0.928

0.736 ± 0.408

5.445 ± 0.826

5.592 ± 1.173

3.753 ± 0.588

7.873 ± 1.162

2.502 ± 0.235

4.047 ± 0.466

5.445 ± 1.314

7.8 ± 1.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.619 ± 0.295

4.415 ± 0.69

5.96 ± 0.39

4.489 ± 0.613

7.138 ± 1.07

6.696 ± 0.989

7.285 ± 1.333

4.857 ± 0.726

1.251 ± 0.318

3.385 ± 0.592
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski