Flavobacterium phage vB_FspS_hattifnatt9-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hattifnattvirus; Flavobacterium virus Hattifnatt

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9L9M7|A0A6B9L9M7_9CAUD DNA methyltransferase OS=Flavobacterium phage vB_FspS_hattifnatt9-1 OX=2686246 GN=hattifnatt91_gp037 PE=4 SV=1
MM1 pKa = 7.13TKK3 pKa = 10.25FVIMKK8 pKa = 7.91TLKK11 pKa = 10.69LLLITALAILFLSCSNDD28 pKa = 3.34DD29 pKa = 4.75NEE31 pKa = 4.51QCTYY35 pKa = 9.76TCNAWVKK42 pKa = 10.29PDD44 pKa = 3.4GQIRR48 pKa = 11.84TVIPVEE54 pKa = 4.25LNCEE58 pKa = 4.08TNEE61 pKa = 4.67PINLPEE67 pKa = 4.47GYY69 pKa = 9.92IFLGCDD75 pKa = 3.18NDD77 pKa = 4.19NIPP80 pKa = 4.55

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LBH3|A0A6B9LBH3_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspS_hattifnatt9-1 OX=2686246 GN=hattifnatt91_gp048 PE=4 SV=1
MM1 pKa = 7.5ILYY4 pKa = 9.48NLSYY8 pKa = 10.05LTMYY12 pKa = 10.72RR13 pKa = 11.84LIVNLISFLIKK24 pKa = 10.04KK25 pKa = 10.26RR26 pKa = 11.84YY27 pKa = 9.38LCLTKK32 pKa = 10.21INNYY36 pKa = 9.93GSIKK40 pKa = 10.27RR41 pKa = 11.84VFPKK45 pKa = 10.48AIRR48 pKa = 11.84RR49 pKa = 11.84NLQPIRR55 pKa = 11.84EE56 pKa = 4.06RR57 pKa = 3.71

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

12407

29

1067

163.3

18.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.965 ± 0.632

1.096 ± 0.195

5.513 ± 0.235

7.036 ± 0.288

5.231 ± 0.28

4.659 ± 0.302

1.435 ± 0.217

8.302 ± 0.337

9.535 ± 0.525

9.076 ± 0.293

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.241 ± 0.156

7.923 ± 0.346

2.345 ± 0.205

3.828 ± 0.214

3.111 ± 0.218

6.569 ± 0.25

6.375 ± 0.375

5.537 ± 0.281

1.007 ± 0.109

4.215 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski