Pyrolobus fumarii (strain DSM 11204 / 1A)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Desulfurococcales; Pyrodictiaceae; Pyrolobus; Pyrolobus fumarii

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1967 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0EFN3|G0EFN3_PYRF1 PilT protein domain protein OS=Pyrolobus fumarii (strain DSM 11204 / 1A) OX=694429 GN=Pyrfu_0332 PE=4 SV=1
MM1 pKa = 7.42IASDD5 pKa = 5.22PIPSVAEE12 pKa = 4.17GMVLLYY18 pKa = 10.11PILTSDD24 pKa = 4.06YY25 pKa = 9.27VSSSSVEE32 pKa = 3.93TMRR35 pKa = 11.84PAEE38 pKa = 4.17LASAILAVHH47 pKa = 6.37VVLQNVGCVEE57 pKa = 3.9ADD59 pKa = 2.99IVYY62 pKa = 10.14LAVGPEE68 pKa = 4.12DD69 pKa = 3.68NNKK72 pKa = 10.0LSNLSSYY79 pKa = 11.13

Molecular weight: 8.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0EEP5|G0EEP5_PYRF1 Glycine cleavage system T protein OS=Pyrolobus fumarii (strain DSM 11204 / 1A) OX=694429 GN=Pyrfu_0999 PE=3 SV=1
MM1 pKa = 7.61ARR3 pKa = 11.84NKK5 pKa = 9.96PLARR9 pKa = 11.84KK10 pKa = 9.11LRR12 pKa = 11.84LAKK15 pKa = 10.02AYY17 pKa = 9.64KK18 pKa = 10.13SNSPVPVWVIVKK30 pKa = 7.15TNRR33 pKa = 11.84RR34 pKa = 11.84FTTHH38 pKa = 6.52PKK40 pKa = 8.18RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.31WRR45 pKa = 11.84RR46 pKa = 11.84NWIKK50 pKa = 10.88AA51 pKa = 3.31

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1967

0

1967

536901

33

1552

273.0

30.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.553 ± 0.066

1.053 ± 0.026

4.323 ± 0.037

7.865 ± 0.073

2.631 ± 0.029

7.501 ± 0.047

1.881 ± 0.026

5.888 ± 0.043

4.512 ± 0.055

11.273 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.167 ± 0.023

2.136 ± 0.033

4.996 ± 0.039

1.716 ± 0.022

8.144 ± 0.073

5.153 ± 0.042

4.497 ± 0.052

9.823 ± 0.058

1.36 ± 0.022

3.527 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski