Amino acid dipepetide frequency for Nitrososphaera gargensis (strain Ga9.2)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.008AlaAla: 9.008 ± 0.131	0.871AlaCys: 0.871 ± 0.039	4.311AlaAsp: 4.311 ± 0.078	5.064AlaGlu: 5.064 ± 0.1	3.178AlaPhe: 3.178 ± 0.065	6.761AlaGly: 6.761 ± 0.111	1.419AlaHis: 1.419 ± 0.044	6.603AlaIle: 6.603 ± 0.108	6.003AlaLys: 6.003 ± 0.098	7.311AlaLeu: 7.311 ± 0.105	2.441AlaMet: 2.441 ± 0.061	2.991AlaAsn: 2.991 ± 0.068	2.58AlaPro: 2.58 ± 0.064	2.452AlaGln: 2.452 ± 0.059	4.468AlaArg: 4.468 ± 0.08	5.621AlaSer: 5.621 ± 0.093	4.591AlaThr: 4.591 ± 0.08	6.171AlaVal: 6.171 ± 0.09	0.721AlaTrp: 0.721 ± 0.033	2.419AlaTyr: 2.419 ± 0.067	0.0AlaXaa: 0.0 ± 0.0
Cys	0.687CysAla: 0.687 ± 0.032	0.21CysCys: 0.21 ± 0.019	0.572CysAsp: 0.572 ± 0.026	0.493CysGlu: 0.493 ± 0.028	0.321CysPhe: 0.321 ± 0.019	1.046CysGly: 1.046 ± 0.038	0.263CysHis: 0.263 ± 0.017	0.69CysIle: 0.69 ± 0.032	0.678CysLys: 0.678 ± 0.032	0.682CysLeu: 0.682 ± 0.03	0.306CysMet: 0.306 ± 0.019	0.447CysAsn: 0.447 ± 0.025	0.565CysPro: 0.565 ± 0.032	0.313CysGln: 0.313 ± 0.023	0.655CysArg: 0.655 ± 0.032	0.733CysSer: 0.733 ± 0.035	0.583CysThr: 0.583 ± 0.028	0.559CysVal: 0.559 ± 0.031	0.094CysTrp: 0.094 ± 0.01	0.392CysTyr: 0.392 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	4.226AspAla: 4.226 ± 0.067	0.495AspCys: 0.495 ± 0.028	3.081AspAsp: 3.081 ± 0.067	3.614AspGlu: 3.614 ± 0.069	2.143AspPhe: 2.143 ± 0.05	3.828AspGly: 3.828 ± 0.082	1.007AspHis: 1.007 ± 0.037	4.428AspIle: 4.428 ± 0.077	3.382AspLys: 3.382 ± 0.065	4.626AspLeu: 4.626 ± 0.084	1.433AspMet: 1.433 ± 0.042	2.167AspAsn: 2.167 ± 0.065	2.321AspPro: 2.321 ± 0.057	1.539AspGln: 1.539 ± 0.049	3.227AspArg: 3.227 ± 0.061	3.74AspSer: 3.74 ± 0.072	2.709AspThr: 2.709 ± 0.062	3.922AspVal: 3.922 ± 0.08	0.568AspTrp: 0.568 ± 0.026	1.975AspTyr: 1.975 ± 0.057	0.0AspXaa: 0.0 ± 0.0
Glu	4.749GluAla: 4.749 ± 0.102	0.575GluCys: 0.575 ± 0.028	2.95GluAsp: 2.95 ± 0.062	4.92GluGlu: 4.92 ± 0.093	2.466GluPhe: 2.466 ± 0.057	3.925GluGly: 3.925 ± 0.076	1.338GluHis: 1.338 ± 0.045	4.917GluIle: 4.917 ± 0.086	5.002GluLys: 5.002 ± 0.101	5.827GluLeu: 5.827 ± 0.092	1.897GluMet: 1.897 ± 0.056	2.704GluAsn: 2.704 ± 0.069	2.225GluPro: 2.225 ± 0.06	2.733GluGln: 2.733 ± 0.075	3.757GluArg: 3.757 ± 0.077	3.704GluSer: 3.704 ± 0.076	2.918GluThr: 2.918 ± 0.067	4.157GluVal: 4.157 ± 0.074	0.645GluTrp: 0.645 ± 0.028	2.093GluTyr: 2.093 ± 0.059	0.0GluXaa: 0.0 ± 0.0
Phe	3.6PheAla: 3.6 ± 0.079	0.478PheCys: 0.478 ± 0.029	2.365PheAsp: 2.365 ± 0.053	2.416PheGlu: 2.416 ± 0.055	1.543PhePhe: 1.543 ± 0.05	3.106PheGly: 3.106 ± 0.063	0.676PheHis: 0.676 ± 0.029	2.399PheIle: 2.399 ± 0.062	2.077PheLys: 2.077 ± 0.055	3.316PheLeu: 3.316 ± 0.081	0.996PheMet: 0.996 ± 0.04	1.444PheAsn: 1.444 ± 0.041	1.489PhePro: 1.489 ± 0.048	1.052PheGln: 1.052 ± 0.038	1.856PheArg: 1.856 ± 0.049	2.801PheSer: 2.801 ± 0.071	2.156PheThr: 2.156 ± 0.056	3.135PheVal: 3.135 ± 0.066	0.42PheTrp: 0.42 ± 0.025	1.421PheTyr: 1.421 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	5.342GlyAla: 5.342 ± 0.097	0.696GlyCys: 0.696 ± 0.03	3.309GlyAsp: 3.309 ± 0.066	3.778GlyGlu: 3.778 ± 0.079	2.934GlyPhe: 2.934 ± 0.068	5.233GlyGly: 5.233 ± 0.124	1.342GlyHis: 1.342 ± 0.048	5.698GlyIle: 5.698 ± 0.095	4.978GlyLys: 4.978 ± 0.096	5.871GlyLeu: 5.871 ± 0.097	2.266GlyMet: 2.266 ± 0.057	2.724GlyAsn: 2.724 ± 0.07	1.908GlyPro: 1.908 ± 0.056	2.103GlyGln: 2.103 ± 0.056	4.105GlyArg: 4.105 ± 0.085	5.12GlySer: 5.12 ± 0.085	3.988GlyThr: 3.988 ± 0.086	4.748GlyVal: 4.748 ± 0.091	0.773GlyTrp: 0.773 ± 0.034	2.455GlyTyr: 2.455 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	1.395HisAla: 1.395 ± 0.047	0.24HisCys: 0.24 ± 0.019	1.102HisAsp: 1.102 ± 0.034	1.174HisGlu: 1.174 ± 0.04	0.824HisPhe: 0.824 ± 0.032	1.478HisGly: 1.478 ± 0.047	0.505HisHis: 0.505 ± 0.03	1.32HisIle: 1.32 ± 0.041	1.016HisLys: 1.016 ± 0.039	1.691HisLeu: 1.691 ± 0.048	0.596HisMet: 0.596 ± 0.027	0.738HisAsn: 0.738 ± 0.029	1.026HisPro: 1.026 ± 0.038	0.542HisGln: 0.542 ± 0.028	1.095HisArg: 1.095 ± 0.04	1.277HisSer: 1.277 ± 0.041	0.964HisThr: 0.964 ± 0.038	1.388HisVal: 1.388 ± 0.048	0.229HisTrp: 0.229 ± 0.017	0.753HisTyr: 0.753 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.821IleAla: 6.821 ± 0.105	0.721IleCys: 0.721 ± 0.032	4.604IleAsp: 4.604 ± 0.077	5.152IleGlu: 5.152 ± 0.08	2.577IlePhe: 2.577 ± 0.061	5.095IleGly: 5.095 ± 0.09	1.283IleHis: 1.283 ± 0.045	5.254IleIle: 5.254 ± 0.103	4.402IleLys: 4.402 ± 0.077	5.961IleLeu: 5.961 ± 0.095	1.988IleMet: 1.988 ± 0.055	2.766IleAsn: 2.766 ± 0.068	3.061IlePro: 3.061 ± 0.068	1.888IleGln: 1.888 ± 0.055	3.815IleArg: 3.815 ± 0.064	4.958IleSer: 4.958 ± 0.084	3.976IleThr: 3.976 ± 0.08	5.658IleVal: 5.658 ± 0.104	0.575IleTrp: 0.575 ± 0.029	2.058IleTyr: 2.058 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	5.176LysAla: 5.176 ± 0.094	0.713LysCys: 0.713 ± 0.031	3.513LysAsp: 3.513 ± 0.084	5.041LysGlu: 5.041 ± 0.1	2.311LysPhe: 2.311 ± 0.051	4.079LysGly: 4.079 ± 0.078	1.194LysHis: 1.194 ± 0.044	4.939LysIle: 4.939 ± 0.076	5.481LysLys: 5.481 ± 0.106	5.346LysLeu: 5.346 ± 0.082	2.305LysMet: 2.305 ± 0.062	2.879LysAsn: 2.879 ± 0.072	2.562LysPro: 2.562 ± 0.057	2.357LysGln: 2.357 ± 0.066	3.636LysArg: 3.636 ± 0.075	4.093LysSer: 4.093 ± 0.086	3.206LysThr: 3.206 ± 0.062	4.978LysVal: 4.978 ± 0.085	0.642LysTrp: 0.642 ± 0.029	2.161LysTyr: 2.161 ± 0.059	0.0LysXaa: 0.0 ± 0.0
Leu	8.696LeuAla: 8.696 ± 0.109	0.757LeuCys: 0.757 ± 0.037	5.103LeuAsp: 5.103 ± 0.078	5.592LeuGlu: 5.592 ± 0.09	3.256LeuPhe: 3.256 ± 0.071	5.869LeuGly: 5.869 ± 0.106	1.764LeuHis: 1.764 ± 0.052	5.12LeuIle: 5.12 ± 0.095	5.659LeuLys: 5.659 ± 0.091	7.922LeuLeu: 7.922 ± 0.136	2.235LeuMet: 2.235 ± 0.057	3.077LeuAsn: 3.077 ± 0.06	3.781LeuPro: 3.781 ± 0.073	3.28LeuGln: 3.28 ± 0.073	4.608LeuArg: 4.608 ± 0.084	6.109LeuSer: 6.109 ± 0.102	4.25LeuThr: 4.25 ± 0.078	6.698LeuVal: 6.698 ± 0.104	0.761LeuTrp: 0.761 ± 0.035	2.63LeuTyr: 2.63 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	2.531MetAla: 2.531 ± 0.059	0.188MetCys: 0.188 ± 0.016	1.333MetAsp: 1.333 ± 0.038	1.485MetGlu: 1.485 ± 0.041	0.96MetPhe: 0.96 ± 0.039	1.704MetGly: 1.704 ± 0.053	0.61MetHis: 0.61 ± 0.029	2.069MetIle: 2.069 ± 0.057	1.914MetLys: 1.914 ± 0.056	2.972MetLeu: 2.972 ± 0.064	0.966MetMet: 0.966 ± 0.042	1.1MetAsn: 1.1 ± 0.04	1.563MetPro: 1.563 ± 0.049	1.24MetGln: 1.24 ± 0.039	1.427MetArg: 1.427 ± 0.045	2.089MetSer: 2.089 ± 0.049	1.732MetThr: 1.732 ± 0.053	2.099MetVal: 2.099 ± 0.052	0.242MetTrp: 0.242 ± 0.018	0.82MetTyr: 0.82 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.239AsnAla: 3.239 ± 0.07	0.425AsnCys: 0.425 ± 0.026	2.174AsnAsp: 2.174 ± 0.063	2.342AsnGlu: 2.342 ± 0.059	1.489AsnPhe: 1.489 ± 0.052	2.687AsnGly: 2.687 ± 0.071	0.723AsnHis: 0.723 ± 0.03	3.042AsnIle: 3.042 ± 0.063	2.299AsnLys: 2.299 ± 0.058	3.325AsnLeu: 3.325 ± 0.063	1.151AsnMet: 1.151 ± 0.04	2.414AsnAsn: 2.414 ± 0.063	2.021AsnPro: 2.021 ± 0.055	1.192AsnGln: 1.192 ± 0.043	2.017AsnArg: 2.017 ± 0.053	2.626AsnSer: 2.626 ± 0.063	2.007AsnThr: 2.007 ± 0.058	2.813AsnVal: 2.813 ± 0.065	0.4AsnTrp: 0.4 ± 0.023	1.33AsnTyr: 1.33 ± 0.044	0.0AsnXaa: 0.0 ± 0.0
Pro	3.477ProAla: 3.477 ± 0.068	0.336ProCys: 0.336 ± 0.024	2.573ProAsp: 2.573 ± 0.065	2.918ProGlu: 2.918 ± 0.066	1.646ProPhe: 1.646 ± 0.05	2.28ProGly: 2.28 ± 0.058	0.845ProHis: 0.845 ± 0.032	2.484ProIle: 2.484 ± 0.058	2.381ProLys: 2.381 ± 0.053	3.46ProLeu: 3.46 ± 0.058	0.864ProMet: 0.864 ± 0.037	1.449ProAsn: 1.449 ± 0.045	1.731ProPro: 1.731 ± 0.056	1.246ProGln: 1.246 ± 0.041	1.786ProArg: 1.786 ± 0.051	2.869ProSer: 2.869 ± 0.064	2.11ProThr: 2.11 ± 0.06	3.305ProVal: 3.305 ± 0.072	0.427ProTrp: 0.427 ± 0.024	1.345ProTyr: 1.345 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	2.63GlnAla: 2.63 ± 0.064	0.285GlnCys: 0.285 ± 0.021	1.625GlnAsp: 1.625 ± 0.05	2.193GlnGlu: 2.193 ± 0.057	1.26GlnPhe: 1.26 ± 0.039	1.739GlnGly: 1.739 ± 0.049	0.682GlnHis: 0.682 ± 0.03	2.309GlnIle: 2.309 ± 0.066	2.451GlnLys: 2.451 ± 0.056	3.001GlnLeu: 3.001 ± 0.071	1.13GlnMet: 1.13 ± 0.044	1.371GlnAsn: 1.371 ± 0.043	1.219GlnPro: 1.219 ± 0.043	1.773GlnGln: 1.773 ± 0.059	1.72GlnArg: 1.72 ± 0.046	2.103GlnSer: 2.103 ± 0.058	1.687GlnThr: 1.687 ± 0.054	2.511GlnVal: 2.511 ± 0.063	0.336GlnTrp: 0.336 ± 0.021	1.169GlnTyr: 1.169 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	3.801ArgAla: 3.801 ± 0.077	0.575ArgCys: 0.575 ± 0.026	2.711ArgAsp: 2.711 ± 0.064	3.472ArgGlu: 3.472 ± 0.075	2.103ArgPhe: 2.103 ± 0.053	3.111ArgGly: 3.111 ± 0.06	1.193ArgHis: 1.193 ± 0.043	4.181ArgIle: 4.181 ± 0.078	4.068ArgLys: 4.068 ± 0.08	5.143ArgLeu: 5.143 ± 0.101	1.902ArgMet: 1.902 ± 0.052	2.207ArgAsn: 2.207 ± 0.064	1.919ArgPro: 1.919 ± 0.052	2.25ArgGln: 2.25 ± 0.058	3.39ArgArg: 3.39 ± 0.071	3.283ArgSer: 3.283 ± 0.079	2.625ArgThr: 2.625 ± 0.062	3.412ArgVal: 3.412 ± 0.061	0.609ArgTrp: 0.609 ± 0.022	2.049ArgTyr: 2.049 ± 0.057	0.0ArgXaa: 0.0 ± 0.0
Ser	5.389SerAla: 5.389 ± 0.085	0.711SerCys: 0.711 ± 0.03	3.732SerAsp: 3.732 ± 0.067	4.045SerGlu: 4.045 ± 0.081	2.806SerPhe: 2.806 ± 0.065	5.353SerGly: 5.353 ± 0.091	1.189SerHis: 1.189 ± 0.044	5.054SerIle: 5.054 ± 0.093	4.654SerLys: 4.654 ± 0.087	6.016SerLeu: 6.016 ± 0.083	2.052SerMet: 2.052 ± 0.049	2.629SerAsn: 2.629 ± 0.059	2.495SerPro: 2.495 ± 0.06	2.082SerGln: 2.082 ± 0.05	3.606SerArg: 3.606 ± 0.068	5.646SerSer: 5.646 ± 0.111	3.696SerThr: 3.696 ± 0.084	4.533SerVal: 4.533 ± 0.086	0.746SerTrp: 0.746 ± 0.03	2.157SerTyr: 2.157 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	4.527ThrAla: 4.527 ± 0.085	0.543ThrCys: 0.543 ± 0.027	2.683ThrAsp: 2.683 ± 0.063	2.892ThrGlu: 2.892 ± 0.064	2.192ThrPhe: 2.192 ± 0.053	4.34ThrGly: 4.34 ± 0.081	0.93ThrHis: 0.93 ± 0.035	4.233ThrIle: 4.233 ± 0.081	3.234ThrLys: 3.234 ± 0.055	4.527ThrLeu: 4.527 ± 0.088	1.465ThrMet: 1.465 ± 0.038	1.888ThrAsn: 1.888 ± 0.051	2.275ThrPro: 2.275 ± 0.06	1.324ThrGln: 1.324 ± 0.038	2.476ThrArg: 2.476 ± 0.059	3.621ThrSer: 3.621 ± 0.07	3.643ThrThr: 3.643 ± 0.09	4.128ThrVal: 4.128 ± 0.089	0.506ThrTrp: 0.506 ± 0.026	1.646ThrTyr: 1.646 ± 0.056	0.0ThrXaa: 0.0 ± 0.0
Val	6.634ValAla: 6.634 ± 0.107	0.819ValCys: 0.819 ± 0.038	4.127ValAsp: 4.127 ± 0.078	4.546ValGlu: 4.546 ± 0.066	2.803ValPhe: 2.803 ± 0.072	4.741ValGly: 4.741 ± 0.082	1.349ValHis: 1.349 ± 0.042	5.231ValIle: 5.231 ± 0.086	4.588ValLys: 4.588 ± 0.082	6.377ValLeu: 6.377 ± 0.1	1.906ValMet: 1.906 ± 0.051	2.743ValAsn: 2.743 ± 0.063	3.062ValPro: 3.062 ± 0.07	2.296ValGln: 2.296 ± 0.055	3.852ValArg: 3.852 ± 0.074	5.136ValSer: 5.136 ± 0.092	4.102ValThr: 4.102 ± 0.083	5.807ValVal: 5.807 ± 0.104	0.653ValTrp: 0.653 ± 0.033	2.241ValTyr: 2.241 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.571TrpAla: 0.571 ± 0.026	0.119TrpCys: 0.119 ± 0.014	0.462TrpAsp: 0.462 ± 0.024	0.478TrpGlu: 0.478 ± 0.022	0.473TrpPhe: 0.473 ± 0.028	0.581TrpGly: 0.581 ± 0.032	0.262TrpHis: 0.262 ± 0.018	0.741TrpIle: 0.741 ± 0.036	0.688TrpLys: 0.688 ± 0.029	0.995TrpLeu: 0.995 ± 0.041	0.333TrpMet: 0.333 ± 0.023	0.491TrpAsn: 0.491 ± 0.026	0.328TrpPro: 0.328 ± 0.021	0.476TrpGln: 0.476 ± 0.028	0.474TrpArg: 0.474 ± 0.025	0.667TrpSer: 0.667 ± 0.034	0.547TrpThr: 0.547 ± 0.029	0.671TrpVal: 0.671 ± 0.032	0.137TrpTrp: 0.137 ± 0.013	0.39TrpTyr: 0.39 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.482TyrAla: 2.482 ± 0.051	0.531TyrCys: 0.531 ± 0.021	2.103TyrAsp: 2.103 ± 0.049	1.886TyrGlu: 1.886 ± 0.051	1.458TyrPhe: 1.458 ± 0.053	2.412TyrGly: 2.412 ± 0.058	0.782TyrHis: 0.782 ± 0.03	1.901TyrIle: 1.901 ± 0.051	1.659TyrLys: 1.659 ± 0.046	2.801TyrLeu: 2.801 ± 0.065	0.811TyrMet: 0.811 ± 0.029	1.488TyrAsn: 1.488 ± 0.045	1.4TyrPro: 1.4 ± 0.045	1.058TyrGln: 1.058 ± 0.038	2.033TyrArg: 2.033 ± 0.049	2.395TyrSer: 2.395 ± 0.059	1.581TyrThr: 1.581 ± 0.052	2.32TyrVal: 2.32 ± 0.059	0.402TyrTrp: 0.402 ± 0.023	1.378TyrTyr: 1.378 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3523 proteins (756973 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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