Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Lymphocryptovirus; Human gammaherpesvirus 4

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03231|GH_EBVB9 Envelope glycoprotein H OS=Epstein-Barr virus (strain B95-8) OX=10377 GN=gH PE=1 SV=1
MM1 pKa = 7.25EE2 pKa = 6.06HH3 pKa = 7.2DD4 pKa = 4.84LEE6 pKa = 5.12RR7 pKa = 11.84GPPGPRR13 pKa = 11.84RR14 pKa = 11.84PPRR17 pKa = 11.84GPPLSSSLGLALLLLLLALLFWLYY41 pKa = 10.78IVMSDD46 pKa = 3.13WTGGALLVLYY56 pKa = 10.32SFALMLIIIILIIFIFRR73 pKa = 11.84RR74 pKa = 11.84DD75 pKa = 3.73LLCPLGALCILLLMITLLLIALWNLHH101 pKa = 4.99GQALFLGIVLFIFGCLLVLGIWIYY125 pKa = 10.72LLEE128 pKa = 4.15MLWRR132 pKa = 11.84LGATIWQLLAFFLAFFLDD150 pKa = 5.18LILLIIALYY159 pKa = 9.75LQQNWWTLLVDD170 pKa = 5.12LLWLLLFLAILIWMYY185 pKa = 10.53YY186 pKa = 8.99HH187 pKa = 6.85GQRR190 pKa = 11.84HH191 pKa = 5.39SDD193 pKa = 3.35EE194 pKa = 4.43HH195 pKa = 7.75HH196 pKa = 6.96HH197 pKa = 7.46DD198 pKa = 4.28DD199 pKa = 5.28SLPHH203 pKa = 6.25PQQATDD209 pKa = 3.84DD210 pKa = 4.24SGHH213 pKa = 6.22EE214 pKa = 4.08SDD216 pKa = 4.7SNSNEE221 pKa = 3.73GRR223 pKa = 11.84HH224 pKa = 5.73HH225 pKa = 6.63LLVSGAGDD233 pKa = 4.11GPPLCSQNLGAPGGGPDD250 pKa = 5.41NGPQDD255 pKa = 4.7PDD257 pKa = 3.47NTDD260 pKa = 3.56DD261 pKa = 5.97NGPQDD266 pKa = 5.0PDD268 pKa = 3.47NTDD271 pKa = 3.47DD272 pKa = 5.12NGPHH276 pKa = 7.25DD277 pKa = 5.7PLPQDD282 pKa = 4.74PDD284 pKa = 3.54NTDD287 pKa = 3.56DD288 pKa = 5.97NGPQDD293 pKa = 5.0PDD295 pKa = 3.47NTDD298 pKa = 3.47DD299 pKa = 5.12NGPHH303 pKa = 7.22DD304 pKa = 5.4PLPHH308 pKa = 6.43SPSDD312 pKa = 3.48SAGNDD317 pKa = 3.34GGPPQLTEE325 pKa = 3.78EE326 pKa = 4.43VEE328 pKa = 4.41NKK330 pKa = 10.57GGDD333 pKa = 3.37QGPPLMTDD341 pKa = 3.13GGGGHH346 pKa = 5.9SHH348 pKa = 7.88DD349 pKa = 4.68SGHH352 pKa = 6.96GGGDD356 pKa = 3.16PHH358 pKa = 8.56LPTLLLGSSGSGGDD372 pKa = 4.23DD373 pKa = 4.27DD374 pKa = 6.1DD375 pKa = 4.11PHH377 pKa = 8.7GPVQLSYY384 pKa = 11.75YY385 pKa = 10.41DD386 pKa = 3.38

Molecular weight: 41.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P25939|TRM1_EBVB9 Tripartite terminase subunit 1 OS=Epstein-Barr virus (strain B95-8) OX=10377 GN=TRM1 PE=3 SV=2
MM1 pKa = 7.61LAHH4 pKa = 7.23LNQVTRR10 pKa = 11.84IPPCPPFSGRR20 pKa = 11.84EE21 pKa = 3.88ARR23 pKa = 11.84LKK25 pKa = 10.34FHH27 pKa = 6.85FFSWSTFMLSWPNNATLRR45 pKa = 11.84EE46 pKa = 3.95IRR48 pKa = 11.84TRR50 pKa = 11.84AATNLTHH57 pKa = 6.93HH58 pKa = 6.79PHH60 pKa = 6.72LVDD63 pKa = 3.51TLYY66 pKa = 10.67HH67 pKa = 7.07ASPQTPFLTRR77 pKa = 11.84SGALYY82 pKa = 10.56RR83 pKa = 11.84FVTCCNCTLPNISIQQCKK101 pKa = 10.23AGDD104 pKa = 3.57RR105 pKa = 11.84PGDD108 pKa = 3.69LEE110 pKa = 6.7IILQSNGGGRR120 pKa = 11.84PASFQFPSSPTGSLLRR136 pKa = 11.84CIVAASLLPEE146 pKa = 4.09VSVGHH151 pKa = 5.82QEE153 pKa = 4.19LSPLRR158 pKa = 11.84SRR160 pKa = 11.84SQGGQTDD167 pKa = 3.87VRR169 pKa = 11.84SGPDD173 pKa = 3.1PARR176 pKa = 11.84RR177 pKa = 11.84LVALLRR183 pKa = 11.84RR184 pKa = 11.84EE185 pKa = 4.23DD186 pKa = 4.14GAPKK190 pKa = 10.26DD191 pKa = 3.82PPLGPFGHH199 pKa = 7.02PRR201 pKa = 11.84GPGPAKK207 pKa = 10.74SEE209 pKa = 4.16DD210 pKa = 3.81EE211 pKa = 3.97EE212 pKa = 4.85SEE214 pKa = 4.53RR215 pKa = 11.84RR216 pKa = 11.84DD217 pKa = 3.74APPPPLDD224 pKa = 3.71SSFQASRR231 pKa = 11.84LVPVGPGFRR240 pKa = 11.84LLVFNTNRR248 pKa = 11.84VINTKK253 pKa = 9.58LVCSEE258 pKa = 4.65PLVKK262 pKa = 9.9MRR264 pKa = 11.84VCNVPRR270 pKa = 11.84LINNFVARR278 pKa = 11.84KK279 pKa = 8.91YY280 pKa = 9.69VVKK283 pKa = 9.23EE284 pKa = 3.69TAFTVSLFFTDD295 pKa = 4.55GVGANLAINVNISGTYY311 pKa = 10.4LSFLLAMTSLRR322 pKa = 11.84CFLPVEE328 pKa = 5.02AIYY331 pKa = 9.19PAAVSNWNSTLDD343 pKa = 3.49LHH345 pKa = 6.85GLEE348 pKa = 4.26NQSLVRR354 pKa = 11.84EE355 pKa = 4.13NRR357 pKa = 11.84SGVFWTTNFPSVVSCRR373 pKa = 11.84DD374 pKa = 3.27GLNVSWFKK382 pKa = 11.05AATATISRR390 pKa = 11.84VHH392 pKa = 5.72GQTLEE397 pKa = 3.64QHH399 pKa = 7.74LIRR402 pKa = 11.84EE403 pKa = 4.04ITPIVTHH410 pKa = 6.79RR411 pKa = 11.84EE412 pKa = 3.93AKK414 pKa = 9.45ISRR417 pKa = 11.84IKK419 pKa = 10.86NRR421 pKa = 11.84LFTLLEE427 pKa = 3.94LRR429 pKa = 11.84NRR431 pKa = 11.84SQIQVLHH438 pKa = 6.05KK439 pKa = 10.55RR440 pKa = 11.84FLEE443 pKa = 4.21GLLDD447 pKa = 4.03CASLLRR453 pKa = 11.84LDD455 pKa = 4.26PSCINRR461 pKa = 11.84IASEE465 pKa = 4.21GLFDD469 pKa = 4.39FSKK472 pKa = 10.9RR473 pKa = 11.84SIAHH477 pKa = 5.1SKK479 pKa = 9.41NRR481 pKa = 11.84HH482 pKa = 4.08EE483 pKa = 4.59CALLGHH489 pKa = 6.33RR490 pKa = 11.84HH491 pKa = 5.44SANVTKK497 pKa = 10.75LVVNEE502 pKa = 4.0RR503 pKa = 11.84KK504 pKa = 7.87TRR506 pKa = 11.84LDD508 pKa = 3.04ILGRR512 pKa = 11.84NANFLTRR519 pKa = 11.84CKK521 pKa = 10.32HH522 pKa = 4.71QVNLRR527 pKa = 11.84QSPIFLTLLRR537 pKa = 11.84HH538 pKa = 4.86IRR540 pKa = 11.84RR541 pKa = 11.84RR542 pKa = 11.84LGLGRR547 pKa = 11.84ASVKK551 pKa = 10.64RR552 pKa = 11.84EE553 pKa = 3.54ITLLLAHH560 pKa = 6.77LRR562 pKa = 11.84KK563 pKa = 8.68KK564 pKa = 7.99TAPIHH569 pKa = 6.52CRR571 pKa = 11.84DD572 pKa = 3.63AQVV575 pKa = 2.73

Molecular weight: 64.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

10

95

46367

39

3149

488.1

53.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.356 ± 0.48

1.945 ± 0.141

4.331 ± 0.162

5.137 ± 0.18

3.384 ± 0.208

7.906 ± 0.563

2.519 ± 0.095

3.311 ± 0.198

2.892 ± 0.169

10.05 ± 0.387

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.799 ± 0.122

2.741 ± 0.2

9.66 ± 0.766

4.096 ± 0.167

7.1 ± 0.34

7.697 ± 0.202

6.004 ± 0.316

6.33 ± 0.248

1.184 ± 0.065

2.558 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski