Amino acid dipepetide frequency for Mycobacterium phage MOOREtheMARYer

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.339AlaAla: 19.339 ± 1.734
0.549AlaCys: 0.549 ± 0.188
8.298AlaAsp: 8.298 ± 0.824
8.847AlaGlu: 8.847 ± 1.501
4.732AlaPhe: 4.732 ± 0.658
14.127AlaGly: 14.127 ± 1.393
2.057AlaHis: 2.057 ± 0.324
6.858AlaIle: 6.858 ± 0.586
5.486AlaLys: 5.486 ± 0.511
11.658AlaLeu: 11.658 ± 1.29
3.017AlaMet: 3.017 ± 0.542
4.183AlaAsn: 4.183 ± 0.531
7.064AlaPro: 7.064 ± 0.854
4.938AlaGln: 4.938 ± 0.565
7.681AlaArg: 7.681 ± 0.828
4.595AlaSer: 4.595 ± 0.787
6.789AlaThr: 6.789 ± 0.633
6.995AlaVal: 6.995 ± 0.713
1.92AlaTrp: 1.92 ± 0.326
2.126AlaTyr: 2.126 ± 0.427
0.0AlaXaa: 0.0 ± 0.0
Cys
0.892CysAla: 0.892 ± 0.266
0.069CysCys: 0.069 ± 0.077
0.48CysAsp: 0.48 ± 0.196
0.754CysGlu: 0.754 ± 0.254
0.411CysPhe: 0.411 ± 0.172
1.029CysGly: 1.029 ± 0.354
0.069CysHis: 0.069 ± 0.063
0.48CysIle: 0.48 ± 0.169
0.069CysLys: 0.069 ± 0.067
0.343CysLeu: 0.343 ± 0.14
0.069CysMet: 0.069 ± 0.062
0.206CysAsn: 0.206 ± 0.13
0.754CysPro: 0.754 ± 0.252
0.274CysGln: 0.274 ± 0.142
1.166CysArg: 1.166 ± 0.321
0.48CysSer: 0.48 ± 0.161
0.411CysThr: 0.411 ± 0.219
0.549CysVal: 0.549 ± 0.219
0.206CysTrp: 0.206 ± 0.146
0.137CysTyr: 0.137 ± 0.106
0.0CysXaa: 0.0 ± 0.0
Asp
9.807AspAla: 9.807 ± 1.278
0.892AspCys: 0.892 ± 0.365
7.132AspAsp: 7.132 ± 0.807
4.938AspGlu: 4.938 ± 0.839
1.92AspPhe: 1.92 ± 0.366
6.858AspGly: 6.858 ± 0.737
1.646AspHis: 1.646 ± 0.381
1.029AspIle: 1.029 ± 0.282
1.509AspLys: 1.509 ± 0.327
4.389AspLeu: 4.389 ± 0.647
0.96AspMet: 0.96 ± 0.232
1.989AspAsn: 1.989 ± 0.348
4.8AspPro: 4.8 ± 0.689
3.36AspGln: 3.36 ± 0.556
5.212AspArg: 5.212 ± 0.696
1.989AspSer: 1.989 ± 0.384
3.703AspThr: 3.703 ± 0.499
5.966AspVal: 5.966 ± 0.732
1.509AspTrp: 1.509 ± 0.348
2.606AspTyr: 2.606 ± 0.42
0.0AspXaa: 0.0 ± 0.0
Glu
7.132GluAla: 7.132 ± 1.184
0.48GluCys: 0.48 ± 0.188
4.046GluAsp: 4.046 ± 0.622
3.635GluGlu: 3.635 ± 0.549
1.852GluPhe: 1.852 ± 0.409
3.84GluGly: 3.84 ± 0.375
1.989GluHis: 1.989 ± 0.385
2.057GluIle: 2.057 ± 0.417
2.469GluLys: 2.469 ± 0.402
3.635GluLeu: 3.635 ± 0.493
0.892GluMet: 0.892 ± 0.265
1.646GluAsn: 1.646 ± 0.32
4.115GluPro: 4.115 ± 0.752
2.469GluGln: 2.469 ± 0.471
4.869GluArg: 4.869 ± 0.963
1.577GluSer: 1.577 ± 0.328
2.675GluThr: 2.675 ± 0.452
3.909GluVal: 3.909 ± 0.565
1.234GluTrp: 1.234 ± 0.247
1.029GluTyr: 1.029 ± 0.298
0.0GluXaa: 0.0 ± 0.0
Phe
3.978PheAla: 3.978 ± 0.412
0.48PheCys: 0.48 ± 0.175
2.675PheAsp: 2.675 ± 0.366
3.155PheGlu: 3.155 ± 0.496
0.411PhePhe: 0.411 ± 0.156
2.949PheGly: 2.949 ± 0.495
0.549PheHis: 0.549 ± 0.199
0.48PheIle: 0.48 ± 0.166
0.892PheLys: 0.892 ± 0.249
1.92PheLeu: 1.92 ± 0.352
0.549PheMet: 0.549 ± 0.227
0.892PheAsn: 0.892 ± 0.23
1.509PhePro: 1.509 ± 0.301
0.823PheGln: 0.823 ± 0.211
1.303PheArg: 1.303 ± 0.274
1.646PheSer: 1.646 ± 0.287
1.509PheThr: 1.509 ± 0.374
2.537PheVal: 2.537 ± 0.392
0.617PheTrp: 0.617 ± 0.302
0.892PheTyr: 0.892 ± 0.263
0.0PheXaa: 0.0 ± 0.0
Gly
11.11GlyAla: 11.11 ± 1.569
0.617GlyCys: 0.617 ± 0.192
6.241GlyAsp: 6.241 ± 0.747
4.046GlyGlu: 4.046 ± 0.495
3.497GlyPhe: 3.497 ± 0.503
12.55GlyGly: 12.55 ± 3.647
2.057GlyHis: 2.057 ± 0.388
4.115GlyIle: 4.115 ± 0.615
4.115GlyLys: 4.115 ± 0.721
6.515GlyLeu: 6.515 ± 0.768
1.714GlyMet: 1.714 ± 0.318
3.84GlyAsn: 3.84 ± 0.722
3.703GlyPro: 3.703 ± 0.537
2.88GlyGln: 2.88 ± 0.599
5.761GlyArg: 5.761 ± 0.686
4.115GlySer: 4.115 ± 0.645
6.515GlyThr: 6.515 ± 0.858
5.966GlyVal: 5.966 ± 1.071
1.92GlyTrp: 1.92 ± 0.405
2.812GlyTyr: 2.812 ± 0.624
0.0GlyXaa: 0.0 ± 0.0
His
2.057HisAla: 2.057 ± 0.47
0.206HisCys: 0.206 ± 0.13
1.234HisAsp: 1.234 ± 0.33
0.343HisGlu: 0.343 ± 0.141
0.343HisPhe: 0.343 ± 0.15
1.783HisGly: 1.783 ± 0.298
0.274HisHis: 0.274 ± 0.153
0.686HisIle: 0.686 ± 0.239
0.274HisLys: 0.274 ± 0.126
1.303HisLeu: 1.303 ± 0.292
0.686HisMet: 0.686 ± 0.216
0.48HisAsn: 0.48 ± 0.201
1.852HisPro: 1.852 ± 0.427
1.234HisGln: 1.234 ± 0.26
2.4HisArg: 2.4 ± 0.469
1.166HisSer: 1.166 ± 0.325
1.234HisThr: 1.234 ± 0.346
1.783HisVal: 1.783 ± 0.314
0.137HisTrp: 0.137 ± 0.09
0.411HisTyr: 0.411 ± 0.182
0.0HisXaa: 0.0 ± 0.0
Ile
6.241IleAla: 6.241 ± 0.595
0.274IleCys: 0.274 ± 0.139
3.772IleAsp: 3.772 ± 0.602
2.4IleGlu: 2.4 ± 0.459
0.754IlePhe: 0.754 ± 0.232
4.938IleGly: 4.938 ± 0.931
1.029IleHis: 1.029 ± 0.257
1.714IleIle: 1.714 ± 0.441
1.097IleLys: 1.097 ± 0.371
2.675IleLeu: 2.675 ± 0.335
0.686IleMet: 0.686 ± 0.336
0.823IleAsn: 0.823 ± 0.256
3.292IlePro: 3.292 ± 0.599
1.372IleGln: 1.372 ± 0.298
3.292IleArg: 3.292 ± 0.463
1.372IleSer: 1.372 ± 0.341
1.92IleThr: 1.92 ± 0.419
3.36IleVal: 3.36 ± 0.37
0.892IleTrp: 0.892 ± 0.243
0.617IleTyr: 0.617 ± 0.185
0.0IleXaa: 0.0 ± 0.0
Lys
5.418LysAla: 5.418 ± 0.543
0.48LysCys: 0.48 ± 0.22
1.577LysAsp: 1.577 ± 0.505
0.823LysGlu: 0.823 ± 0.252
1.166LysPhe: 1.166 ± 0.291
2.126LysGly: 2.126 ± 0.419
0.823LysHis: 0.823 ± 0.203
1.714LysIle: 1.714 ± 0.328
0.892LysLys: 0.892 ± 0.275
2.812LysLeu: 2.812 ± 0.437
1.166LysMet: 1.166 ± 0.208
0.96LysAsn: 0.96 ± 0.284
2.4LysPro: 2.4 ± 0.411
0.892LysGln: 0.892 ± 0.269
3.36LysArg: 3.36 ± 0.583
1.714LysSer: 1.714 ± 0.343
2.469LysThr: 2.469 ± 0.541
2.057LysVal: 2.057 ± 0.41
0.617LysTrp: 0.617 ± 0.196
0.686LysTyr: 0.686 ± 0.247
0.0LysXaa: 0.0 ± 0.0
Leu
8.504LeuAla: 8.504 ± 0.831
0.617LeuCys: 0.617 ± 0.249
6.378LeuAsp: 6.378 ± 0.784
3.36LeuGlu: 3.36 ± 0.5
2.537LeuPhe: 2.537 ± 0.491
7.544LeuGly: 7.544 ± 1.616
0.96LeuHis: 0.96 ± 0.231
2.949LeuIle: 2.949 ± 0.393
2.4LeuLys: 2.4 ± 0.472
5.898LeuLeu: 5.898 ± 0.614
1.44LeuMet: 1.44 ± 0.496
2.4LeuAsn: 2.4 ± 0.347
4.32LeuPro: 4.32 ± 0.652
1.577LeuGln: 1.577 ± 0.409
5.28LeuArg: 5.28 ± 0.613
4.732LeuSer: 4.732 ± 0.608
5.006LeuThr: 5.006 ± 0.502
5.006LeuVal: 5.006 ± 0.557
1.303LeuTrp: 1.303 ± 0.353
1.783LeuTyr: 1.783 ± 0.362
0.0LeuXaa: 0.0 ± 0.0
Met
3.429MetAla: 3.429 ± 0.53
0.137MetCys: 0.137 ± 0.1
1.166MetAsp: 1.166 ± 0.239
1.166MetGlu: 1.166 ± 0.242
0.411MetPhe: 0.411 ± 0.178
1.372MetGly: 1.372 ± 0.319
0.206MetHis: 0.206 ± 0.108
1.372MetIle: 1.372 ± 0.342
0.96MetLys: 0.96 ± 0.236
1.509MetLeu: 1.509 ± 0.339
0.411MetMet: 0.411 ± 0.16
0.411MetAsn: 0.411 ± 0.152
1.303MetPro: 1.303 ± 0.24
0.686MetGln: 0.686 ± 0.21
1.44MetArg: 1.44 ± 0.361
1.714MetSer: 1.714 ± 0.295
2.4MetThr: 2.4 ± 0.347
1.029MetVal: 1.029 ± 0.228
0.206MetTrp: 0.206 ± 0.141
0.137MetTyr: 0.137 ± 0.095
0.0MetXaa: 0.0 ± 0.0
Asn
3.84AsnAla: 3.84 ± 0.564
0.411AsnCys: 0.411 ± 0.257
1.989AsnAsp: 1.989 ± 0.365
1.44AsnGlu: 1.44 ± 0.379
1.097AsnPhe: 1.097 ± 0.277
3.497AsnGly: 3.497 ± 0.626
0.549AsnHis: 0.549 ± 0.179
0.686AsnIle: 0.686 ± 0.211
0.754AsnLys: 0.754 ± 0.216
1.783AsnLeu: 1.783 ± 0.399
0.48AsnMet: 0.48 ± 0.186
0.686AsnAsn: 0.686 ± 0.218
3.635AsnPro: 3.635 ± 0.503
1.029AsnGln: 1.029 ± 0.301
3.017AsnArg: 3.017 ± 0.447
0.754AsnSer: 0.754 ± 0.226
2.332AsnThr: 2.332 ± 0.324
1.646AsnVal: 1.646 ± 0.389
0.823AsnTrp: 0.823 ± 0.227
0.411AsnTyr: 0.411 ± 0.164
0.0AsnXaa: 0.0 ± 0.0
Pro
9.052ProAla: 9.052 ± 0.79
0.274ProCys: 0.274 ± 0.151
6.515ProAsp: 6.515 ± 0.762
3.223ProGlu: 3.223 ± 0.38
1.577ProPhe: 1.577 ± 0.335
4.8ProGly: 4.8 ± 0.539
1.166ProHis: 1.166 ± 0.298
3.223ProIle: 3.223 ± 0.407
2.263ProLys: 2.263 ± 0.356
4.389ProLeu: 4.389 ± 0.499
1.714ProMet: 1.714 ± 0.33
2.126ProAsn: 2.126 ± 0.482
4.183ProPro: 4.183 ± 0.699
1.92ProGln: 1.92 ± 0.381
3.772ProArg: 3.772 ± 0.527
2.88ProSer: 2.88 ± 0.501
4.869ProThr: 4.869 ± 0.66
3.155ProVal: 3.155 ± 0.626
0.823ProTrp: 0.823 ± 0.208
1.166ProTyr: 1.166 ± 0.322
0.0ProXaa: 0.0 ± 0.0
Gln
5.555GlnAla: 5.555 ± 0.6
0.069GlnCys: 0.069 ± 0.072
2.057GlnAsp: 2.057 ± 0.37
0.686GlnGlu: 0.686 ± 0.208
1.097GlnPhe: 1.097 ± 0.242
2.4GlnGly: 2.4 ± 0.466
1.029GlnHis: 1.029 ± 0.289
1.92GlnIle: 1.92 ± 0.329
1.166GlnLys: 1.166 ± 0.261
4.458GlnLeu: 4.458 ± 0.517
1.646GlnMet: 1.646 ± 0.32
1.372GlnAsn: 1.372 ± 0.336
2.4GlnPro: 2.4 ± 0.407
1.783GlnGln: 1.783 ± 0.333
2.332GlnArg: 2.332 ± 0.404
1.577GlnSer: 1.577 ± 0.294
1.714GlnThr: 1.714 ± 0.389
1.646GlnVal: 1.646 ± 0.361
0.549GlnTrp: 0.549 ± 0.168
0.411GlnTyr: 0.411 ± 0.144
0.0GlnXaa: 0.0 ± 0.0
Arg
8.435ArgAla: 8.435 ± 1.144
1.166ArgCys: 1.166 ± 0.299
4.389ArgAsp: 4.389 ± 0.757
4.595ArgGlu: 4.595 ± 0.673
1.577ArgPhe: 1.577 ± 0.371
5.075ArgGly: 5.075 ± 0.544
1.44ArgHis: 1.44 ± 0.364
3.429ArgIle: 3.429 ± 0.41
2.743ArgLys: 2.743 ± 0.534
5.486ArgLeu: 5.486 ± 0.641
2.057ArgMet: 2.057 ± 0.364
1.783ArgAsn: 1.783 ± 0.335
4.526ArgPro: 4.526 ± 0.685
2.949ArgGln: 2.949 ± 0.523
7.406ArgArg: 7.406 ± 1.043
3.292ArgSer: 3.292 ± 0.494
4.938ArgThr: 4.938 ± 0.534
3.703ArgVal: 3.703 ± 0.452
1.577ArgTrp: 1.577 ± 0.438
1.44ArgTyr: 1.44 ± 0.315
0.0ArgXaa: 0.0 ± 0.0
Ser
5.075SerAla: 5.075 ± 0.67
0.343SerCys: 0.343 ± 0.176
3.429SerAsp: 3.429 ± 0.408
2.332SerGlu: 2.332 ± 0.388
1.166SerPhe: 1.166 ± 0.254
4.663SerGly: 4.663 ± 0.854
0.686SerHis: 0.686 ± 0.234
1.989SerIle: 1.989 ± 0.333
2.332SerLys: 2.332 ± 0.331
2.949SerLeu: 2.949 ± 0.663
0.617SerMet: 0.617 ± 0.218
1.577SerAsn: 1.577 ± 0.331
2.88SerPro: 2.88 ± 0.443
1.029SerGln: 1.029 ± 0.266
2.606SerArg: 2.606 ± 0.407
2.949SerSer: 2.949 ± 0.624
3.086SerThr: 3.086 ± 0.552
2.949SerVal: 2.949 ± 0.561
1.097SerTrp: 1.097 ± 0.272
1.234SerTyr: 1.234 ± 0.241
0.0SerXaa: 0.0 ± 0.0
Thr
8.709ThrAla: 8.709 ± 0.758
0.549ThrCys: 0.549 ± 0.188
4.252ThrAsp: 4.252 ± 0.588
3.292ThrGlu: 3.292 ± 0.447
2.057ThrPhe: 2.057 ± 0.441
5.692ThrGly: 5.692 ± 0.639
1.234ThrHis: 1.234 ± 0.242
2.743ThrIle: 2.743 ± 0.473
2.126ThrLys: 2.126 ± 0.362
3.909ThrLeu: 3.909 ± 0.614
1.783ThrMet: 1.783 ± 0.444
2.537ThrAsn: 2.537 ± 0.407
4.8ThrPro: 4.8 ± 0.518
1.989ThrGln: 1.989 ± 0.424
3.635ThrArg: 3.635 ± 0.499
3.703ThrSer: 3.703 ± 0.472
3.909ThrThr: 3.909 ± 0.54
4.115ThrVal: 4.115 ± 0.559
0.892ThrTrp: 0.892 ± 0.191
1.029ThrTyr: 1.029 ± 0.236
0.0ThrXaa: 0.0 ± 0.0
Val
7.886ValAla: 7.886 ± 1.121
0.823ValCys: 0.823 ± 0.236
4.8ValAsp: 4.8 ± 0.483
4.458ValGlu: 4.458 ± 0.664
1.989ValPhe: 1.989 ± 0.384
5.555ValGly: 5.555 ± 0.712
0.823ValHis: 0.823 ± 0.205
3.429ValIle: 3.429 ± 0.397
1.646ValLys: 1.646 ± 0.354
4.526ValLeu: 4.526 ± 0.52
1.097ValMet: 1.097 ± 0.276
1.783ValAsn: 1.783 ± 0.336
3.017ValPro: 3.017 ± 0.362
2.4ValGln: 2.4 ± 0.488
4.663ValArg: 4.663 ± 0.598
2.469ValSer: 2.469 ± 0.412
4.8ValThr: 4.8 ± 0.8
5.761ValVal: 5.761 ± 0.61
1.989ValTrp: 1.989 ± 0.39
1.509ValTyr: 1.509 ± 0.333
0.0ValXaa: 0.0 ± 0.0
Trp
1.989TrpAla: 1.989 ± 0.337
0.137TrpCys: 0.137 ± 0.089
0.892TrpAsp: 0.892 ± 0.221
1.029TrpGlu: 1.029 ± 0.234
0.892TrpPhe: 0.892 ± 0.24
1.509TrpGly: 1.509 ± 0.368
0.617TrpHis: 0.617 ± 0.204
1.029TrpIle: 1.029 ± 0.284
0.549TrpLys: 0.549 ± 0.211
1.92TrpLeu: 1.92 ± 0.452
0.274TrpMet: 0.274 ± 0.126
0.686TrpAsn: 0.686 ± 0.2
1.097TrpPro: 1.097 ± 0.213
0.96TrpGln: 0.96 ± 0.227
1.44TrpArg: 1.44 ± 0.458
0.96TrpSer: 0.96 ± 0.328
1.577TrpThr: 1.577 ± 0.391
1.166TrpVal: 1.166 ± 0.265
0.549TrpTrp: 0.549 ± 0.192
0.411TrpTyr: 0.411 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.812TyrAla: 2.812 ± 0.671
0.274TyrCys: 0.274 ± 0.157
0.892TyrAsp: 0.892 ± 0.221
1.029TyrGlu: 1.029 ± 0.27
0.274TyrPhe: 0.274 ± 0.14
1.852TyrGly: 1.852 ± 0.355
0.686TyrHis: 0.686 ± 0.284
0.617TyrIle: 0.617 ± 0.231
0.411TyrLys: 0.411 ± 0.153
1.92TyrLeu: 1.92 ± 0.331
0.137TyrMet: 0.137 ± 0.096
0.48TyrAsn: 0.48 ± 0.142
1.44TyrPro: 1.44 ± 0.279
1.303TyrGln: 1.303 ± 0.333
1.372TyrArg: 1.372 ± 0.32
1.303TyrSer: 1.303 ± 0.346
1.097TyrThr: 1.097 ± 0.298
2.126TyrVal: 2.126 ± 0.423
0.823TyrTrp: 0.823 ± 0.256
0.549TyrTyr: 0.549 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (14583 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski