Helicobacter phage FrGC43A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; unclassified Schmidvirus

Average proteome isoelectric point is 7.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5RFZ7|A0A1S5RFZ7_9CAUD Uncharacterized protein OS=Helicobacter phage FrGC43A OX=1852666 PE=4 SV=1
MM1 pKa = 6.63MRR3 pKa = 11.84VVFFDD8 pKa = 3.34ASGVLEE14 pKa = 4.28AFDD17 pKa = 3.72YY18 pKa = 11.04RR19 pKa = 11.84GVLLHH24 pKa = 5.6TQEE27 pKa = 5.21IKK29 pKa = 11.08DD30 pKa = 3.76NLNAKK35 pKa = 10.24LPFTQKK41 pKa = 10.64NFFKK45 pKa = 10.96FNGVSFGVCEE55 pKa = 4.09GVGDD59 pKa = 4.79LDD61 pKa = 3.92YY62 pKa = 11.39RR63 pKa = 11.84DD64 pKa = 3.51YY65 pKa = 11.75SKK67 pKa = 11.41NINFNALLCEE77 pKa = 4.26TIEE80 pKa = 4.7NYY82 pKa = 10.56LLNAKK87 pKa = 9.81EE88 pKa = 4.56PEE90 pKa = 4.15NKK92 pKa = 8.56QQKK95 pKa = 10.21ALLVDD100 pKa = 4.5FLAVYY105 pKa = 9.93DD106 pKa = 3.98KK107 pKa = 11.26NIEE110 pKa = 3.81KK111 pKa = 10.37GFYY114 pKa = 9.62YY115 pKa = 10.74LEE117 pKa = 4.38PPFFKK122 pKa = 10.58EE123 pKa = 5.4KK124 pKa = 10.47EE125 pKa = 3.95IEE127 pKa = 4.06LLNMRR132 pKa = 11.84FEE134 pKa = 4.62TNDD137 pKa = 2.98RR138 pKa = 11.84SS139 pKa = 3.52

Molecular weight: 16.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5RG13|A0A1S5RG13_9CAUD Mobile element protein OS=Helicobacter phage FrGC43A OX=1852666 PE=3 SV=1
MM1 pKa = 7.26RR2 pKa = 11.84HH3 pKa = 5.93KK4 pKa = 11.09NEE6 pKa = 3.95TATSFNKK13 pKa = 10.38LKK15 pKa = 10.5EE16 pKa = 3.8ITLAIQAQKK25 pKa = 11.29NSVQTSDD32 pKa = 3.17NANTSEE38 pKa = 4.24ITSQSTPTPKK48 pKa = 9.58GTARR52 pKa = 11.84AKK54 pKa = 10.74NSALKK59 pKa = 7.59PTKK62 pKa = 9.79RR63 pKa = 11.84AFSEE67 pKa = 4.09RR68 pKa = 11.84QRR70 pKa = 11.84ITIRR74 pKa = 11.84DD75 pKa = 3.61NSQASAKK82 pKa = 9.47EE83 pKa = 4.04IKK85 pKa = 10.07RR86 pKa = 11.84GRR88 pKa = 3.52

Molecular weight: 9.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

9868

45

1561

266.7

30.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.425 ± 0.267

0.902 ± 0.233

4.884 ± 0.346

8.492 ± 0.376

4.884 ± 0.251

4.185 ± 0.316

1.348 ± 0.162

7.205 ± 0.304

10.965 ± 0.468

10.377 ± 0.347

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.875 ± 0.182

8.188 ± 0.472

2.219 ± 0.141

4.459 ± 0.368

3.871 ± 0.232

6.668 ± 0.232

4.712 ± 0.332

4.185 ± 0.358

0.476 ± 0.077

3.679 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski