Crocuta crocuta (Spotted hyena)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Carnivora; Feliformia

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16983 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G1A633|A0A6G1A633_CROCR LORF2 protein (Fragment) OS=Crocuta crocuta OX=9678 GN=Pol_626 PE=4 SV=1
RR1 pKa = 8.59SVDD4 pKa = 3.96FLASGAQSIPNGNPARR20 pKa = 11.84GEE22 pKa = 4.32GPHH25 pKa = 7.36SEE27 pKa = 4.24EE28 pKa = 3.88EE29 pKa = 4.27DD30 pKa = 3.59YY31 pKa = 12.0AMEE34 pKa = 6.07DD35 pKa = 3.94DD36 pKa = 6.17DD37 pKa = 6.68SDD39 pKa = 5.05GDD41 pKa = 3.79LSAWEE46 pKa = 4.05LSEE49 pKa = 4.96GVSEE53 pKa = 5.06CPPKK57 pKa = 10.52EE58 pKa = 3.75QAADD62 pKa = 3.96LFNEE66 pKa = 4.26DD67 pKa = 3.11WDD69 pKa = 4.81LEE71 pKa = 4.38LKK73 pKa = 10.49ADD75 pKa = 3.94QGNPYY80 pKa = 10.37DD81 pKa = 4.73ADD83 pKa = 5.04DD84 pKa = 3.86IQGCVSQEE92 pKa = 3.63AKK94 pKa = 10.34PWVCCAPQGDD104 pKa = 4.66MIYY107 pKa = 10.59DD108 pKa = 4.29PSWHH112 pKa = 6.9HH113 pKa = 6.75PPPLIPHH120 pKa = 5.6YY121 pKa = 10.76SKK123 pKa = 10.49MVFEE127 pKa = 5.05TGQFDD132 pKa = 4.21DD133 pKa = 6.01AEE135 pKa = 4.35DD136 pKa = 3.31

Molecular weight: 15.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G1ACY7|A0A6G1ACY7_CROCR PMS1 protein (Fragment) OS=Crocuta crocuta OX=9678 GN=Pms1 PE=3 SV=1
VV1 pKa = 7.19RR2 pKa = 11.84APTVPVRR9 pKa = 11.84APTVPVRR16 pKa = 11.84APTVPVRR23 pKa = 11.84APTVPVRR30 pKa = 11.84APTVPARR37 pKa = 11.84TPTVPVNTPTVPTRR51 pKa = 11.84APTVPAHH58 pKa = 6.57IPTVPAHH65 pKa = 5.89TPTVPVHH72 pKa = 5.89TPTVPVRR79 pKa = 11.84TPTVPVRR86 pKa = 11.84APTVPVRR93 pKa = 11.84APTVPARR100 pKa = 11.84APTVLAHH107 pKa = 6.25TPTVPVRR114 pKa = 11.84ARR116 pKa = 11.84TVPVRR121 pKa = 11.84APTVPVHH128 pKa = 6.08TPTIPVRR135 pKa = 11.84APTVPARR142 pKa = 11.84APIVPAHH149 pKa = 6.4IPIVPAHH156 pKa = 5.38TT157 pKa = 3.59

Molecular weight: 16.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16983

0

16983

8071222

50

27055

475.3

53.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.837 ± 0.022

2.299 ± 0.016

4.759 ± 0.016

6.985 ± 0.029

3.796 ± 0.014

6.362 ± 0.027

2.601 ± 0.01

4.669 ± 0.018

5.881 ± 0.03

10.032 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.011

3.626 ± 0.015

6.085 ± 0.024

4.573 ± 0.019

5.603 ± 0.018

8.073 ± 0.027

5.39 ± 0.017

6.076 ± 0.023

1.336 ± 0.007

2.784 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski