Providencia phage Redjac

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4HZG8|K4HZG8_9CAUD Uncharacterized protein OS=Providencia phage Redjac OX=1235559 GN=Redjac_0270 PE=4 SV=1
MM1 pKa = 7.68SYY3 pKa = 10.86ADD5 pKa = 4.78CVAALPKK12 pKa = 10.48EE13 pKa = 4.4SVNRR17 pKa = 11.84DD18 pKa = 2.88IYY20 pKa = 11.32AVSCTRR26 pKa = 11.84GEE28 pKa = 4.11PLEE31 pKa = 4.59SEE33 pKa = 4.45

Molecular weight: 3.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4I436|K4I436_9CAUD Conserved tail assembly protein OS=Providencia phage Redjac OX=1235559 GN=Redjac_0010 PE=4 SV=1
MM1 pKa = 8.1PINIVTIGSKK11 pKa = 10.32EE12 pKa = 3.87LGDD15 pKa = 3.96YY16 pKa = 10.89LEE18 pKa = 4.4QVPKK22 pKa = 10.38IANQSIRR29 pKa = 11.84MAINTVATGKK39 pKa = 10.7GMSKK43 pKa = 9.83IKK45 pKa = 10.29RR46 pKa = 11.84AMLDD50 pKa = 3.21EE51 pKa = 4.23VAFPSGYY58 pKa = 10.55LSGDD62 pKa = 3.51RR63 pKa = 11.84LRR65 pKa = 11.84VSKK68 pKa = 10.7KK69 pKa = 9.26ATGTNLEE76 pKa = 4.43AIILARR82 pKa = 11.84KK83 pKa = 9.19RR84 pKa = 11.84ATSLARR90 pKa = 11.84FVTGGAMVSNSKK102 pKa = 10.56RR103 pKa = 11.84PGGVQVRR110 pKa = 11.84VKK112 pKa = 10.42RR113 pKa = 11.84GKK115 pKa = 8.65TSYY118 pKa = 10.5LKK120 pKa = 10.42NAFIVRR126 pKa = 11.84LNKK129 pKa = 10.08GASLTEE135 pKa = 3.88DD136 pKa = 3.39QYY138 pKa = 12.25NLGLAVRR145 pKa = 11.84LSAGEE150 pKa = 3.92KK151 pKa = 10.34LNNKK155 pKa = 6.19TTQHH159 pKa = 6.42KK160 pKa = 9.68SWLVPGRR167 pKa = 11.84VALLYY172 pKa = 10.61GPSVDD177 pKa = 3.62QVFSSVSDD185 pKa = 3.48KK186 pKa = 10.81LAPAIADD193 pKa = 3.43DD194 pKa = 4.05VAAEE198 pKa = 3.89FHH200 pKa = 7.16RR201 pKa = 11.84NFEE204 pKa = 4.41RR205 pKa = 11.84LSKK208 pKa = 10.94

Molecular weight: 22.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

14168

33

1435

345.6

38.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.387 ± 0.557

0.748 ± 0.12

6.578 ± 0.335

6.642 ± 0.294

3.508 ± 0.184

7.319 ± 0.379

1.659 ± 0.179

5.668 ± 0.163

5.534 ± 0.443

7.164 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.049 ± 0.133

4.15 ± 0.231

4.792 ± 0.42

3.903 ± 0.414

5.329 ± 0.181

6.239 ± 0.325

6.148 ± 0.304

7.023 ± 0.361

1.715 ± 0.182

3.444 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski