Dishui Lake virophage 6

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; unclassified Lavidaviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XMP1|A0A6G6XMP1_9VIRU Putative cysteine protease OS=Dishui Lake virophage 6 OX=2704067 PE=4 SV=1
MM1 pKa = 7.6SFDD4 pKa = 3.75TFCGQNRR11 pKa = 11.84TDD13 pKa = 3.27PSTYY17 pKa = 10.49QKK19 pKa = 11.23VLTKK23 pKa = 10.15FLSAAPTAVIDD34 pKa = 3.9TTNANATVFPVLAAGGGGLPQQLLVDD60 pKa = 5.07DD61 pKa = 4.96GLTPISVNCFNGNFNVVDD79 pKa = 3.92TLKK82 pKa = 9.25ITQTQVAVGKK92 pKa = 10.29GAGATNQAADD102 pKa = 3.78SVAVGIGAGTTNQGTQCVAVGEE124 pKa = 4.45ASGQSNQGANATAVGVVAGQINQGANATALGVNAGNSNQSANSVAVGLNAGEE176 pKa = 4.38TTQGADD182 pKa = 2.82SVAIGNVAGQTTQGASSVAIGINAGNTTQGASSVAVGSSAGQTTQGANSVAVGLNAGNTTQGVNATALGSNAANDD257 pKa = 3.8SQGNNGVAIGLNAGQTTQGADD278 pKa = 2.98AVAIGTNAGQTTQTLQAVAIGVNAGVTTQGANSVAIGAASGFTTQGANSVSIGLSSGTTNQGTQSVAVGADD349 pKa = 3.07SGGTNQGASSVAVGYY364 pKa = 8.88FAGGSAQGGSSVAIGVNAGRR384 pKa = 11.84TTQGDD389 pKa = 3.3NSVAVGGGAGQTTQGAGAVCIGSNAGNGNQGANAVAIGNLAGQTNQSAGSIVINASGAALNTGATVGLFIKK460 pKa = 9.74PIRR463 pKa = 11.84GVALGIGAGRR473 pKa = 11.84LFYY476 pKa = 10.88DD477 pKa = 3.71VATSEE482 pKa = 4.27IQYY485 pKa = 8.53STTT488 pKa = 3.12

Molecular weight: 45.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XMI1|A0A6G6XMI1_9VIRU Putative major capsid protein OS=Dishui Lake virophage 6 OX=2704067 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 4.18FTSTLIEE9 pKa = 4.01KK10 pKa = 10.32LKK12 pKa = 10.8EE13 pKa = 3.94RR14 pKa = 11.84GLTDD18 pKa = 3.07SSVALYY24 pKa = 10.23VRR26 pKa = 11.84NLEE29 pKa = 4.34KK30 pKa = 11.04LNSNKK35 pKa = 9.68PLNNLNFLKK44 pKa = 10.29KK45 pKa = 10.88YY46 pKa = 9.87NDD48 pKa = 2.99IMEE51 pKa = 4.49KK52 pKa = 10.62LKK54 pKa = 10.78GYY56 pKa = 9.93KK57 pKa = 10.54GNTQRR62 pKa = 11.84GFLISIVSSLSSFKK76 pKa = 10.89GEE78 pKa = 4.13KK79 pKa = 10.68GIDD82 pKa = 3.6PLLKK86 pKa = 9.99KK87 pKa = 10.21YY88 pKa = 9.66YY89 pKa = 10.48KK90 pKa = 11.18AMIEE94 pKa = 4.2LNKK97 pKa = 10.52SLNEE101 pKa = 4.01ANHH104 pKa = 6.43NGLKK108 pKa = 9.71TEE110 pKa = 4.18TQSANWLDD118 pKa = 3.79WNDD121 pKa = 3.23VEE123 pKa = 5.86HH124 pKa = 7.59IYY126 pKa = 11.13DD127 pKa = 3.99GLRR130 pKa = 11.84DD131 pKa = 4.51NITQMSSPITEE142 pKa = 4.05GEE144 pKa = 4.13YY145 pKa = 10.89NRR147 pKa = 11.84LLDD150 pKa = 4.28LVVLSLYY157 pKa = 10.39VLNPPRR163 pKa = 11.84RR164 pKa = 11.84NSDD167 pKa = 3.56YY168 pKa = 10.78MNMKK172 pKa = 9.44VVSAFTPEE180 pKa = 3.82VSDD183 pKa = 4.07ALSGNNILDD192 pKa = 3.64WNGKK196 pKa = 8.69RR197 pKa = 11.84FIFRR201 pKa = 11.84NYY203 pKa = 8.17KK204 pKa = 6.84TAKK207 pKa = 10.0KK208 pKa = 9.37YY209 pKa = 11.2GEE211 pKa = 4.53TIVPIPRR218 pKa = 11.84EE219 pKa = 4.11LYY221 pKa = 10.57DD222 pKa = 3.27ILAVYY227 pKa = 10.05FDD229 pKa = 3.45KK230 pKa = 11.14RR231 pKa = 11.84GILRR235 pKa = 11.84RR236 pKa = 11.84LQVPQKK242 pKa = 8.85KK243 pKa = 7.31TKK245 pKa = 9.94KK246 pKa = 8.15EE247 pKa = 3.48ASIFIEE253 pKa = 4.44PFLTLWNDD261 pKa = 3.03KK262 pKa = 10.34PFLINTITRR271 pKa = 11.84ILNRR275 pKa = 11.84VFGKK279 pKa = 10.66KK280 pKa = 9.32IGSSMLRR287 pKa = 11.84HH288 pKa = 6.69IYY290 pKa = 6.2TTKK293 pKa = 10.92KK294 pKa = 10.15FGKK297 pKa = 9.61QLAEE301 pKa = 3.96QKK303 pKa = 8.52EE304 pKa = 4.34TAEE307 pKa = 4.23KK308 pKa = 9.32MGHH311 pKa = 5.79SVAEE315 pKa = 4.13MNQTYY320 pKa = 10.18IKK322 pKa = 10.52EE323 pKa = 4.17DD324 pKa = 3.15

Molecular weight: 37.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5679

143

952

473.3

52.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.924 ± 0.916

0.845 ± 0.199

5.494 ± 0.347

6.515 ± 0.825

3.751 ± 0.301

7.096 ± 0.958

1.109 ± 0.207

6.41 ± 0.543

6.057 ± 0.951

8.382 ± 0.42

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.06 ± 0.225

6.498 ± 0.543

5.089 ± 0.683

4.825 ± 0.87

5.917 ± 0.898

6.374 ± 0.675

5.23 ± 0.637

6.11 ± 0.415

0.898 ± 0.279

3.416 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski