Prochlorococcus phage MED4-213

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Eurybiavirus; Prochlorococcus virus MED4-213

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 215 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4QPZ5|M4QPZ5_9CAUD Uncharacterized protein OS=Prochlorococcus phage MED4-213 OX=889956 GN=CPMG_00054 PE=4 SV=1
MM1 pKa = 7.92TDD3 pKa = 3.4QQSLEE8 pKa = 4.08YY9 pKa = 10.77DD10 pKa = 3.04ADD12 pKa = 3.57AVMEE16 pKa = 3.85QLIMQEE22 pKa = 3.96SRR24 pKa = 11.84HH25 pKa = 4.78MQMGALVEE33 pKa = 4.18HH34 pKa = 6.31QLMYY38 pKa = 10.75PDD40 pKa = 3.21MTIRR44 pKa = 11.84DD45 pKa = 4.53FFDD48 pKa = 3.02MAATEE53 pKa = 3.96LRR55 pKa = 11.84EE56 pKa = 4.01QEE58 pKa = 4.37EE59 pKa = 4.05EE60 pKa = 3.91LGYY63 pKa = 11.11YY64 pKa = 10.01DD65 pKa = 4.4

Molecular weight: 7.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4QH70|M4QH70_9CAUD Uncharacterized protein OS=Prochlorococcus phage MED4-213 OX=889956 GN=CPMG_00131 PE=4 SV=1
MM1 pKa = 7.17KK2 pKa = 10.06RR3 pKa = 11.84KK4 pKa = 9.62SGRR7 pKa = 11.84KK8 pKa = 8.23MKK10 pKa = 10.49EE11 pKa = 3.02FDD13 pKa = 3.37YY14 pKa = 11.48GLDD17 pKa = 3.33YY18 pKa = 10.08KK19 pKa = 11.09TIDD22 pKa = 3.45FTVKK26 pKa = 10.14EE27 pKa = 3.96NRR29 pKa = 11.84KK30 pKa = 9.34LYY32 pKa = 10.38RR33 pKa = 11.84IGRR36 pKa = 11.84GEE38 pKa = 3.92QGVLLVRR45 pKa = 11.84PYY47 pKa = 11.04TNLICAHH54 pKa = 5.29WRR56 pKa = 11.84FVNEE60 pKa = 3.63DD61 pKa = 3.39VARR64 pKa = 11.84KK65 pKa = 8.85SASKK69 pKa = 9.78IYY71 pKa = 11.27SMFCDD76 pKa = 3.52YY77 pKa = 10.93KK78 pKa = 10.49KK79 pKa = 10.65QRR81 pKa = 11.84DD82 pKa = 4.39FIGMDD87 pKa = 3.29MARR90 pKa = 11.84KK91 pKa = 8.21FLEE94 pKa = 3.86MGFTRR99 pKa = 11.84SRR101 pKa = 11.84RR102 pKa = 11.84YY103 pKa = 10.18ANHH106 pKa = 7.06PSGKK110 pKa = 9.83KK111 pKa = 7.79YY112 pKa = 10.84ARR114 pKa = 11.84DD115 pKa = 3.66GSISPQSPTALHH127 pKa = 6.38CEE129 pKa = 4.23KK130 pKa = 10.7SRR132 pKa = 11.84SATVFKK138 pKa = 10.94KK139 pKa = 9.98MRR141 pKa = 11.84DD142 pKa = 3.06RR143 pKa = 11.84AAYY146 pKa = 10.18DD147 pKa = 3.07EE148 pKa = 4.83TYY150 pKa = 10.84QKK152 pKa = 10.71LRR154 pKa = 11.84KK155 pKa = 8.19QWRR158 pKa = 11.84SEE160 pKa = 3.88EE161 pKa = 4.05

Molecular weight: 19.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

215

0

215

56990

50

4458

265.1

29.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.873 ± 0.216

0.907 ± 0.067

6.696 ± 0.133

5.754 ± 0.333

4.269 ± 0.094

7.68 ± 0.34

1.683 ± 0.108

6.324 ± 0.135

6.015 ± 0.437

6.97 ± 0.194

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.095 ± 0.19

5.878 ± 0.214

3.792 ± 0.133

3.716 ± 0.099

3.776 ± 0.127

7.263 ± 0.277

8.201 ± 0.498

6.703 ± 0.167

1.112 ± 0.082

4.292 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski