Amino acid dipepetide frequency for Streptomyces phage SV1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
27.386AlaAla: 27.386 ± 2.383
1.099AlaCys: 1.099 ± 0.311
8.875AlaAsp: 8.875 ± 0.739
11.664AlaGlu: 11.664 ± 1.404
1.352AlaPhe: 1.352 ± 0.286
11.242AlaGly: 11.242 ± 0.942
3.043AlaHis: 3.043 ± 0.494
5.071AlaIle: 5.071 ± 0.581
2.874AlaLys: 2.874 ± 0.603
12.34AlaLeu: 12.34 ± 1.243
3.381AlaMet: 3.381 ± 0.696
1.944AlaAsn: 1.944 ± 0.42
10.143AlaPro: 10.143 ± 1.256
5.832AlaGln: 5.832 ± 0.83
15.045AlaArg: 15.045 ± 1.323
6.001AlaSer: 6.001 ± 0.754
9.213AlaThr: 9.213 ± 0.874
11.833AlaVal: 11.833 ± 0.697
1.944AlaTrp: 1.944 ± 0.357
3.55AlaTyr: 3.55 ± 0.519
0.0AlaXaa: 0.0 ± 0.0
Cys
0.761CysAla: 0.761 ± 0.265
0.085CysCys: 0.085 ± 0.078
0.676CysAsp: 0.676 ± 0.237
0.761CysGlu: 0.761 ± 0.241
0.085CysPhe: 0.085 ± 0.088
0.507CysGly: 0.507 ± 0.246
0.085CysHis: 0.085 ± 0.09
0.169CysIle: 0.169 ± 0.116
0.423CysLys: 0.423 ± 0.215
0.423CysLeu: 0.423 ± 0.203
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.761CysPro: 0.761 ± 0.291
0.0CysGln: 0.0 ± 0.0
1.437CysArg: 1.437 ± 0.456
0.085CysSer: 0.085 ± 0.09
0.338CysThr: 0.338 ± 0.143
0.338CysVal: 0.338 ± 0.159
0.0CysTrp: 0.0 ± 0.0
0.085CysTyr: 0.085 ± 0.087
0.0CysXaa: 0.0 ± 0.0
Asp
8.706AspAla: 8.706 ± 0.99
0.085AspCys: 0.085 ± 0.079
5.071AspAsp: 5.071 ± 1.044
4.902AspGlu: 4.902 ± 0.634
0.592AspPhe: 0.592 ± 0.237
6.424AspGly: 6.424 ± 0.856
1.268AspHis: 1.268 ± 0.348
1.944AspIle: 1.944 ± 0.43
0.592AspLys: 0.592 ± 0.246
6.086AspLeu: 6.086 ± 0.856
0.93AspMet: 0.93 ± 0.244
0.761AspAsn: 0.761 ± 0.237
5.494AspPro: 5.494 ± 0.666
2.874AspGln: 2.874 ± 0.458
7.776AspArg: 7.776 ± 1.201
2.198AspSer: 2.198 ± 0.402
3.212AspThr: 3.212 ± 0.57
4.395AspVal: 4.395 ± 0.652
1.099AspTrp: 1.099 ± 0.337
0.845AspTyr: 0.845 ± 0.254
0.0AspXaa: 0.0 ± 0.0
Glu
7.523GluAla: 7.523 ± 0.958
0.676GluCys: 0.676 ± 0.22
2.705GluAsp: 2.705 ± 0.437
2.62GluGlu: 2.62 ± 0.488
1.521GluPhe: 1.521 ± 0.454
4.226GluGly: 4.226 ± 0.554
2.198GluHis: 2.198 ± 0.533
1.69GluIle: 1.69 ± 0.301
1.268GluLys: 1.268 ± 0.334
3.973GluLeu: 3.973 ± 0.712
1.099GluMet: 1.099 ± 0.429
1.014GluAsn: 1.014 ± 0.244
3.719GluPro: 3.719 ± 0.566
6.086GluGln: 6.086 ± 0.886
4.311GluArg: 4.311 ± 0.67
1.86GluSer: 1.86 ± 0.449
3.888GluThr: 3.888 ± 0.628
3.465GluVal: 3.465 ± 0.566
2.198GluTrp: 2.198 ± 0.468
1.183GluTyr: 1.183 ± 0.21
0.0GluXaa: 0.0 ± 0.0
Phe
2.029PheAla: 2.029 ± 0.43
0.085PheCys: 0.085 ± 0.09
1.606PheAsp: 1.606 ± 0.305
0.676PheGlu: 0.676 ± 0.258
0.254PhePhe: 0.254 ± 0.14
1.86PheGly: 1.86 ± 0.469
0.169PheHis: 0.169 ± 0.127
0.761PheIle: 0.761 ± 0.188
0.254PheLys: 0.254 ± 0.179
1.014PheLeu: 1.014 ± 0.285
0.169PheMet: 0.169 ± 0.104
0.423PheAsn: 0.423 ± 0.219
0.676PhePro: 0.676 ± 0.265
0.423PheGln: 0.423 ± 0.163
2.029PheArg: 2.029 ± 0.574
1.014PheSer: 1.014 ± 0.328
1.014PheThr: 1.014 ± 0.279
0.507PheVal: 0.507 ± 0.246
0.169PheTrp: 0.169 ± 0.126
0.169PheTyr: 0.169 ± 0.12
0.0PheXaa: 0.0 ± 0.0
Gly
9.974GlyAla: 9.974 ± 1.217
0.254GlyCys: 0.254 ± 0.151
5.156GlyAsp: 5.156 ± 0.633
5.325GlyGlu: 5.325 ± 0.686
1.606GlyPhe: 1.606 ± 0.52
5.663GlyGly: 5.663 ± 0.872
1.437GlyHis: 1.437 ± 0.342
4.142GlyIle: 4.142 ± 0.672
1.944GlyLys: 1.944 ± 0.414
7.354GlyLeu: 7.354 ± 1.057
1.69GlyMet: 1.69 ± 0.359
1.775GlyAsn: 1.775 ± 0.322
6.508GlyPro: 6.508 ± 1.461
2.367GlyGln: 2.367 ± 0.491
8.03GlyArg: 8.03 ± 0.76
4.057GlySer: 4.057 ± 0.54
6.508GlyThr: 6.508 ± 0.997
4.48GlyVal: 4.48 ± 0.562
2.029GlyTrp: 2.029 ± 0.467
2.705GlyTyr: 2.705 ± 0.437
0.0GlyXaa: 0.0 ± 0.0
His
2.367HisAla: 2.367 ± 0.511
0.085HisCys: 0.085 ± 0.088
1.437HisAsp: 1.437 ± 0.327
0.761HisGlu: 0.761 ± 0.287
0.761HisPhe: 0.761 ± 0.284
1.944HisGly: 1.944 ± 0.498
0.423HisHis: 0.423 ± 0.175
0.592HisIle: 0.592 ± 0.231
0.338HisLys: 0.338 ± 0.161
1.86HisLeu: 1.86 ± 0.441
0.085HisMet: 0.085 ± 0.089
0.338HisAsn: 0.338 ± 0.173
1.86HisPro: 1.86 ± 0.412
0.423HisGln: 0.423 ± 0.177
2.958HisArg: 2.958 ± 0.456
0.93HisSer: 0.93 ± 0.343
1.437HisThr: 1.437 ± 0.356
1.69HisVal: 1.69 ± 0.368
0.507HisTrp: 0.507 ± 0.223
0.254HisTyr: 0.254 ± 0.134
0.0HisXaa: 0.0 ± 0.0
Ile
4.733IleAla: 4.733 ± 0.828
0.169IleCys: 0.169 ± 0.142
2.113IleAsp: 2.113 ± 0.497
2.536IleGlu: 2.536 ± 0.545
0.423IlePhe: 0.423 ± 0.155
3.043IleGly: 3.043 ± 0.742
0.676IleHis: 0.676 ± 0.226
1.352IleIle: 1.352 ± 0.353
0.676IleLys: 0.676 ± 0.299
2.536IleLeu: 2.536 ± 0.365
0.507IleMet: 0.507 ± 0.243
0.845IleAsn: 0.845 ± 0.291
2.789IlePro: 2.789 ± 0.484
1.352IleGln: 1.352 ± 0.359
2.874IleArg: 2.874 ± 0.483
1.521IleSer: 1.521 ± 0.422
3.127IleThr: 3.127 ± 0.435
2.789IleVal: 2.789 ± 0.476
0.423IleTrp: 0.423 ± 0.189
0.761IleTyr: 0.761 ± 0.289
0.0IleXaa: 0.0 ± 0.0
Lys
3.465LysAla: 3.465 ± 0.722
0.254LysCys: 0.254 ± 0.154
0.845LysAsp: 0.845 ± 0.281
1.014LysGlu: 1.014 ± 0.331
0.254LysPhe: 0.254 ± 0.152
2.282LysGly: 2.282 ± 0.545
0.423LysHis: 0.423 ± 0.174
0.93LysIle: 0.93 ± 0.367
0.676LysLys: 0.676 ± 0.276
0.93LysLeu: 0.93 ± 0.319
0.254LysMet: 0.254 ± 0.138
0.592LysAsn: 0.592 ± 0.221
1.775LysPro: 1.775 ± 0.797
0.254LysGln: 0.254 ± 0.143
1.268LysArg: 1.268 ± 0.361
1.352LysSer: 1.352 ± 0.344
1.099LysThr: 1.099 ± 0.31
0.93LysVal: 0.93 ± 0.295
0.254LysTrp: 0.254 ± 0.147
0.423LysTyr: 0.423 ± 0.173
0.0LysXaa: 0.0 ± 0.0
Leu
16.144LeuAla: 16.144 ± 1.508
0.507LeuCys: 0.507 ± 0.19
9.551LeuAsp: 9.551 ± 0.999
2.62LeuGlu: 2.62 ± 0.535
1.521LeuPhe: 1.521 ± 0.298
5.832LeuGly: 5.832 ± 0.809
1.437LeuHis: 1.437 ± 0.333
3.127LeuIle: 3.127 ± 0.525
1.183LeuLys: 1.183 ± 0.299
7.269LeuLeu: 7.269 ± 0.796
1.099LeuMet: 1.099 ± 0.442
1.352LeuAsn: 1.352 ± 0.309
5.071LeuPro: 5.071 ± 0.598
1.69LeuGln: 1.69 ± 0.486
5.071LeuArg: 5.071 ± 0.599
3.465LeuSer: 3.465 ± 0.53
6.339LeuThr: 6.339 ± 0.845
4.902LeuVal: 4.902 ± 0.7
1.014LeuTrp: 1.014 ± 0.294
2.029LeuTyr: 2.029 ± 0.361
0.0LeuXaa: 0.0 ± 0.0
Met
2.536MetAla: 2.536 ± 0.58
0.169MetCys: 0.169 ± 0.128
1.183MetAsp: 1.183 ± 0.298
0.507MetGlu: 0.507 ± 0.2
0.085MetPhe: 0.085 ± 0.09
1.183MetGly: 1.183 ± 0.498
0.0MetHis: 0.0 ± 0.0
0.423MetIle: 0.423 ± 0.198
0.676MetLys: 0.676 ± 0.208
1.014MetLeu: 1.014 ± 0.343
0.254MetMet: 0.254 ± 0.152
0.592MetAsn: 0.592 ± 0.215
0.93MetPro: 0.93 ± 0.276
1.268MetGln: 1.268 ± 0.392
1.86MetArg: 1.86 ± 0.453
1.69MetSer: 1.69 ± 0.335
2.198MetThr: 2.198 ± 0.399
0.761MetVal: 0.761 ± 0.278
0.338MetTrp: 0.338 ± 0.181
0.338MetTyr: 0.338 ± 0.193
0.0MetXaa: 0.0 ± 0.0
Asn
2.367AsnAla: 2.367 ± 0.455
0.169AsnCys: 0.169 ± 0.112
1.014AsnAsp: 1.014 ± 0.297
0.845AsnGlu: 0.845 ± 0.252
0.338AsnPhe: 0.338 ± 0.158
2.029AsnGly: 2.029 ± 0.422
0.338AsnHis: 0.338 ± 0.202
0.592AsnIle: 0.592 ± 0.208
0.254AsnLys: 0.254 ± 0.136
1.268AsnLeu: 1.268 ± 0.279
0.761AsnMet: 0.761 ± 0.24
0.338AsnAsn: 0.338 ± 0.196
1.352AsnPro: 1.352 ± 0.482
0.423AsnGln: 0.423 ± 0.162
1.944AsnArg: 1.944 ± 0.509
1.099AsnSer: 1.099 ± 0.28
1.099AsnThr: 1.099 ± 0.365
0.93AsnVal: 0.93 ± 0.343
0.254AsnTrp: 0.254 ± 0.14
0.169AsnTyr: 0.169 ± 0.104
0.0AsnXaa: 0.0 ± 0.0
Pro
12.763ProAla: 12.763 ± 1.151
0.761ProCys: 0.761 ± 0.273
4.733ProAsp: 4.733 ± 0.773
3.973ProGlu: 3.973 ± 0.582
0.676ProPhe: 0.676 ± 0.273
8.03ProGly: 8.03 ± 0.791
1.268ProHis: 1.268 ± 0.318
2.282ProIle: 2.282 ± 0.341
1.86ProLys: 1.86 ± 0.547
3.719ProLeu: 3.719 ± 0.664
1.268ProMet: 1.268 ± 0.361
1.352ProAsn: 1.352 ± 0.277
4.48ProPro: 4.48 ± 0.841
2.198ProGln: 2.198 ± 0.694
5.071ProArg: 5.071 ± 0.768
2.958ProSer: 2.958 ± 0.472
5.579ProThr: 5.579 ± 0.811
4.48ProVal: 4.48 ± 0.701
0.845ProTrp: 0.845 ± 0.259
1.014ProTyr: 1.014 ± 0.286
0.0ProXaa: 0.0 ± 0.0
Gln
5.663GlnAla: 5.663 ± 0.77
0.169GlnCys: 0.169 ± 0.124
1.352GlnAsp: 1.352 ± 0.361
1.944GlnGlu: 1.944 ± 0.413
0.676GlnPhe: 0.676 ± 0.282
2.451GlnGly: 2.451 ± 0.755
1.268GlnHis: 1.268 ± 0.363
0.676GlnIle: 0.676 ± 0.231
0.592GlnLys: 0.592 ± 0.194
3.804GlnLeu: 3.804 ± 0.539
0.507GlnMet: 0.507 ± 0.202
0.507GlnAsn: 0.507 ± 0.242
2.705GlnPro: 2.705 ± 0.646
3.043GlnGln: 3.043 ± 0.654
3.719GlnArg: 3.719 ± 0.679
1.352GlnSer: 1.352 ± 0.298
3.127GlnThr: 3.127 ± 0.382
2.536GlnVal: 2.536 ± 0.543
1.437GlnTrp: 1.437 ± 0.302
0.761GlnTyr: 0.761 ± 0.257
0.0GlnXaa: 0.0 ± 0.0
Arg
15.383ArgAla: 15.383 ± 1.203
0.761ArgCys: 0.761 ± 0.245
5.071ArgAsp: 5.071 ± 0.862
4.226ArgGlu: 4.226 ± 0.777
1.352ArgPhe: 1.352 ± 0.354
5.579ArgGly: 5.579 ± 0.525
2.789ArgHis: 2.789 ± 0.584
3.212ArgIle: 3.212 ± 0.517
2.367ArgLys: 2.367 ± 0.562
9.129ArgLeu: 9.129 ± 0.816
1.437ArgMet: 1.437 ± 0.309
1.944ArgAsn: 1.944 ± 0.347
4.902ArgPro: 4.902 ± 0.818
3.719ArgGln: 3.719 ± 0.85
10.143ArgArg: 10.143 ± 1.262
4.057ArgSer: 4.057 ± 0.71
8.621ArgThr: 8.621 ± 0.898
5.748ArgVal: 5.748 ± 0.809
1.86ArgTrp: 1.86 ± 0.31
1.775ArgTyr: 1.775 ± 0.408
0.0ArgXaa: 0.0 ± 0.0
Ser
4.818SerAla: 4.818 ± 0.629
0.338SerCys: 0.338 ± 0.223
2.029SerAsp: 2.029 ± 0.409
1.521SerGlu: 1.521 ± 0.488
1.352SerPhe: 1.352 ± 0.244
5.748SerGly: 5.748 ± 0.769
0.761SerHis: 0.761 ± 0.21
1.86SerIle: 1.86 ± 0.419
0.338SerLys: 0.338 ± 0.185
4.311SerLeu: 4.311 ± 0.712
0.93SerMet: 0.93 ± 0.296
0.676SerAsn: 0.676 ± 0.251
3.127SerPro: 3.127 ± 0.499
1.69SerGln: 1.69 ± 0.351
3.888SerArg: 3.888 ± 0.693
1.521SerSer: 1.521 ± 0.348
4.142SerThr: 4.142 ± 0.592
3.381SerVal: 3.381 ± 0.494
0.93SerTrp: 0.93 ± 0.247
1.014SerTyr: 1.014 ± 0.28
0.0SerXaa: 0.0 ± 0.0
Thr
13.101ThrAla: 13.101 ± 0.98
0.338ThrCys: 0.338 ± 0.214
3.296ThrAsp: 3.296 ± 0.546
3.381ThrGlu: 3.381 ± 0.527
1.352ThrPhe: 1.352 ± 0.289
6.593ThrGly: 6.593 ± 0.915
1.183ThrHis: 1.183 ± 0.299
2.113ThrIle: 2.113 ± 0.46
1.268ThrLys: 1.268 ± 0.354
5.24ThrLeu: 5.24 ± 0.655
1.099ThrMet: 1.099 ± 0.282
1.352ThrAsn: 1.352 ± 0.326
7.185ThrPro: 7.185 ± 0.781
1.69ThrGln: 1.69 ± 0.472
5.579ThrArg: 5.579 ± 0.599
3.719ThrSer: 3.719 ± 0.597
5.494ThrThr: 5.494 ± 0.75
6.677ThrVal: 6.677 ± 0.829
1.437ThrTrp: 1.437 ± 0.349
1.521ThrTyr: 1.521 ± 0.429
0.0ThrXaa: 0.0 ± 0.0
Val
9.298ValAla: 9.298 ± 0.919
0.676ValCys: 0.676 ± 0.254
5.663ValAsp: 5.663 ± 0.634
5.917ValGlu: 5.917 ± 0.702
0.845ValPhe: 0.845 ± 0.278
5.156ValGly: 5.156 ± 0.758
1.521ValHis: 1.521 ± 0.361
2.874ValIle: 2.874 ± 0.451
1.014ValLys: 1.014 ± 0.279
5.41ValLeu: 5.41 ± 0.541
1.606ValMet: 1.606 ± 0.434
0.93ValAsn: 0.93 ± 0.295
3.973ValPro: 3.973 ± 0.619
1.775ValGln: 1.775 ± 0.38
6.593ValArg: 6.593 ± 0.808
2.958ValSer: 2.958 ± 0.434
4.226ValThr: 4.226 ± 0.75
5.41ValVal: 5.41 ± 0.764
1.521ValTrp: 1.521 ± 0.358
0.592ValTyr: 0.592 ± 0.25
0.0ValXaa: 0.0 ± 0.0
Trp
2.198TrpAla: 2.198 ± 0.506
0.254TrpCys: 0.254 ± 0.146
1.099TrpAsp: 1.099 ± 0.295
1.014TrpGlu: 1.014 ± 0.255
0.085TrpPhe: 0.085 ± 0.08
0.845TrpGly: 0.845 ± 0.239
0.592TrpHis: 0.592 ± 0.285
0.845TrpIle: 0.845 ± 0.295
0.507TrpLys: 0.507 ± 0.284
2.113TrpLeu: 2.113 ± 0.532
0.676TrpMet: 0.676 ± 0.209
0.592TrpAsn: 0.592 ± 0.276
0.676TrpPro: 0.676 ± 0.222
0.845TrpGln: 0.845 ± 0.28
2.029TrpArg: 2.029 ± 0.454
1.437TrpSer: 1.437 ± 0.377
1.944TrpThr: 1.944 ± 0.455
0.761TrpVal: 0.761 ± 0.255
0.254TrpTrp: 0.254 ± 0.123
0.169TrpTyr: 0.169 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.62TyrAla: 2.62 ± 0.524
0.085TyrCys: 0.085 ± 0.079
1.775TyrAsp: 1.775 ± 0.48
1.014TyrGlu: 1.014 ± 0.339
0.169TyrPhe: 0.169 ± 0.135
2.367TyrGly: 2.367 ± 0.518
0.169TyrHis: 0.169 ± 0.111
0.592TyrIle: 0.592 ± 0.248
0.169TyrLys: 0.169 ± 0.138
1.437TyrLeu: 1.437 ± 0.339
0.169TyrMet: 0.169 ± 0.105
0.254TyrAsn: 0.254 ± 0.152
1.268TyrPro: 1.268 ± 0.282
0.338TyrGln: 0.338 ± 0.174
2.451TyrArg: 2.451 ± 0.579
1.099TyrSer: 1.099 ± 0.334
1.014TyrThr: 1.014 ± 0.308
1.944TyrVal: 1.944 ± 0.322
0.423TyrTrp: 0.423 ± 0.145
0.085TyrTyr: 0.085 ± 0.105
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (11832 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski