Bdellovibrio exovorus JSS

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Oligoflexia; Bdellovibrionales; Bdellovibrionaceae; Bdellovibrio; Bdellovibrio exovorus

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2618 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4VS60|M4VS60_9PROT Peptidyl-tRNA hydrolase OS=Bdellovibrio exovorus JSS OX=1184267 GN=pth PE=3 SV=1
MM1 pKa = 7.39KK2 pKa = 9.46PALGLLLGISISIAAFANTEE22 pKa = 4.19SKK24 pKa = 9.77TPFYY28 pKa = 9.46YY29 pKa = 10.0TQGSLKK35 pKa = 10.24SQTVEE40 pKa = 3.78DD41 pKa = 4.3TTFLAYY47 pKa = 9.71EE48 pKa = 5.14GYY50 pKa = 11.04GEE52 pKa = 4.15ACYY55 pKa = 10.64VGNAWKK61 pKa = 10.49ARR63 pKa = 11.84AVLKK67 pKa = 11.18NMANDD72 pKa = 4.87DD73 pKa = 3.77IEE75 pKa = 4.39KK76 pKa = 10.25TNVQVWLDD84 pKa = 3.77QNTGALTFSYY94 pKa = 11.2VDD96 pKa = 3.66TKK98 pKa = 10.95CTDD101 pKa = 3.22DD102 pKa = 3.7SGYY105 pKa = 10.51EE106 pKa = 3.98SEE108 pKa = 4.29EE109 pKa = 4.15AAVEE113 pKa = 4.1ACSVSVVIPACGSS126 pKa = 3.19

Molecular weight: 13.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4VCF5|M4VCF5_9PROT 3-hydroxy-3-methylglutaryl coenzyme A reductase OS=Bdellovibrio exovorus JSS OX=1184267 GN=A11Q_1500 PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.95RR4 pKa = 11.84TYY6 pKa = 9.93QPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 7.87KK15 pKa = 7.69THH17 pKa = 5.22GFRR20 pKa = 11.84SKK22 pKa = 9.56TATAAGRR29 pKa = 11.84KK30 pKa = 8.81LLNRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.05GRR40 pKa = 11.84KK41 pKa = 8.77KK42 pKa = 10.16LTVTSGGKK50 pKa = 9.48

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2618

0

2618

818528

30

1706

312.7

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.691 ± 0.054

0.955 ± 0.018

5.045 ± 0.033

6.383 ± 0.048

4.775 ± 0.041

6.263 ± 0.045

2.03 ± 0.024

6.368 ± 0.04

6.901 ± 0.058

10.067 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.021

4.442 ± 0.035

3.86 ± 0.03

4.546 ± 0.03

4.66 ± 0.032

7.304 ± 0.044

5.357 ± 0.043

6.712 ± 0.044

1.102 ± 0.018

3.173 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski