Neophocaena asiaeorientalis asiaeorientalis (Yangtze finless porpoise) (Neophocaena phocaenoides subsp. asiaeorientalis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Artiodactyla;

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32160 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A341BDA9|A0A341BDA9_NEOAA Isoform of A0A341BDQ3 cystine/glutamate transporter isoform X6 OS=Neophocaena asiaeorientalis asiaeorientalis OX=1706337 GN=SLC7A11 PE=3 SV=1
MM1 pKa = 7.3EE2 pKa = 5.17AVSLVLFVLGLLLLPLLAVLLMALCVRR29 pKa = 11.84CRR31 pKa = 11.84EE32 pKa = 4.2LPGSYY37 pKa = 8.62GTAASDD43 pKa = 3.64GLTPSSIVIKK53 pKa = 9.83PPPTVAPWPPATSYY67 pKa = 11.3PPVTYY72 pKa = 10.68LLQSQPDD79 pKa = 3.91LLRR82 pKa = 11.84IPRR85 pKa = 11.84SPQPPGGSPRR95 pKa = 11.84MPSSQQDD102 pKa = 3.01SDD104 pKa = 3.88GANSVASYY112 pKa = 10.42EE113 pKa = 4.12NEE115 pKa = 3.82EE116 pKa = 4.38AVCEE120 pKa = 4.31DD121 pKa = 3.54ADD123 pKa = 3.94EE124 pKa = 6.05DD125 pKa = 4.37EE126 pKa = 5.92DD127 pKa = 4.86EE128 pKa = 4.15EE129 pKa = 5.22DD130 pKa = 3.81YY131 pKa = 11.56PEE133 pKa = 5.32GYY135 pKa = 10.61LVVLPDD141 pKa = 3.71NVPATGAAVPPAPASSNPGLRR162 pKa = 11.84DD163 pKa = 3.06SAFSMEE169 pKa = 4.23SGEE172 pKa = 4.99DD173 pKa = 3.63YY174 pKa = 11.66VNVPEE179 pKa = 4.68SEE181 pKa = 4.29EE182 pKa = 4.1SADD185 pKa = 3.58ASLDD189 pKa = 3.44GSRR192 pKa = 11.84EE193 pKa = 3.99YY194 pKa = 11.72VNVSQEE200 pKa = 4.06LPPVEE205 pKa = 4.02RR206 pKa = 11.84TKK208 pKa = 10.75PAGRR212 pKa = 11.84SSQEE216 pKa = 3.88VEE218 pKa = 4.13GEE220 pKa = 3.96EE221 pKa = 4.31APDD224 pKa = 3.64YY225 pKa = 11.32EE226 pKa = 4.44NLQIHH231 pKa = 6.6

Molecular weight: 24.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A341C3Z8|A0A341C3Z8_NEOAA cytosolic carboxypeptidase 1 isoform X1 OS=Neophocaena asiaeorientalis asiaeorientalis OX=1706337 GN=AGTPBP1 PE=3 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18229

13931

32160

20602167

32

34356

640.6

71.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.006 ± 0.014

2.15 ± 0.01

4.839 ± 0.009

7.269 ± 0.017

3.528 ± 0.011

6.507 ± 0.018

2.591 ± 0.006

4.195 ± 0.012

5.754 ± 0.017

9.941 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.006

3.548 ± 0.009

6.341 ± 0.023

4.904 ± 0.013

5.769 ± 0.013

8.5 ± 0.018

5.264 ± 0.011

6.013 ± 0.012

1.183 ± 0.004

2.554 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski