Escherichia phage vB_EcoP_PHB19

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; unclassified Studiervirinae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9RKK4|A0A6B9RKK4_9CAUD Terminase large subunit OS=Escherichia phage vB_EcoP_PHB19 OX=2698729 PE=3 SV=1
MM1 pKa = 7.48AMSNMTYY8 pKa = 11.18NNVFDD13 pKa = 4.72HH14 pKa = 6.93AYY16 pKa = 10.49EE17 pKa = 3.86MLKK20 pKa = 10.85EE21 pKa = 4.08NIRR24 pKa = 11.84YY25 pKa = 9.81DD26 pKa = 4.67DD27 pKa = 4.5IRR29 pKa = 11.84DD30 pKa = 3.55TDD32 pKa = 3.94DD33 pKa = 3.47LHH35 pKa = 7.49DD36 pKa = 4.63AVHH39 pKa = 6.2MAADD43 pKa = 3.67NAVPHH48 pKa = 6.36YY49 pKa = 9.52YY50 pKa = 10.64SDD52 pKa = 3.78VFSVMASEE60 pKa = 5.5GIDD63 pKa = 4.5LEE65 pKa = 4.76FEE67 pKa = 4.73DD68 pKa = 5.02SGLMPDD74 pKa = 3.42TKK76 pKa = 11.02DD77 pKa = 3.78VIRR80 pKa = 11.84SLQARR85 pKa = 11.84IYY87 pKa = 9.24EE88 pKa = 4.14QLTIDD93 pKa = 3.64LWEE96 pKa = 4.69DD97 pKa = 3.57AEE99 pKa = 4.86DD100 pKa = 4.13LLNEE104 pKa = 3.98YY105 pKa = 10.49LEE107 pKa = 4.28EE108 pKa = 4.24VEE110 pKa = 4.59EE111 pKa = 4.41SEE113 pKa = 4.44EE114 pKa = 4.19YY115 pKa = 10.91EE116 pKa = 4.28EE117 pKa = 5.76GEE119 pKa = 4.29EE120 pKa = 4.19

Molecular weight: 14.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9RGV4|A0A6B9RGV4_9CAUD DNA ligase OS=Escherichia phage vB_EcoP_PHB19 OX=2698729 PE=4 SV=1
MM1 pKa = 8.04PIHH4 pKa = 6.45TKK6 pKa = 10.62NGATVCTPDD15 pKa = 3.38GFAMKK20 pKa = 10.26QRR22 pKa = 11.84IEE24 pKa = 3.89RR25 pKa = 11.84LKK27 pKa = 10.72RR28 pKa = 11.84EE29 pKa = 3.64LRR31 pKa = 11.84INRR34 pKa = 11.84KK35 pKa = 8.91INNII39 pKa = 3.59

Molecular weight: 4.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12150

30

1318

243.0

27.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.045 ± 0.568

1.119 ± 0.184

6.403 ± 0.229

7.309 ± 0.336

3.588 ± 0.191

7.35 ± 0.331

1.794 ± 0.194

5.391 ± 0.165

6.675 ± 0.409

8.033 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.996 ± 0.198

4.56 ± 0.318

3.407 ± 0.206

3.671 ± 0.305

5.63 ± 0.197

6.222 ± 0.313

5.457 ± 0.306

6.453 ± 0.293

1.588 ± 0.172

3.309 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski