Amino acid dipepetide frequency for Pseudomonas phage PAJU2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.745AlaAla: 14.745 ± 1.848
1.404AlaCys: 1.404 ± 0.305
5.547AlaAsp: 5.547 ± 0.731
8.847AlaGlu: 8.847 ± 1.285
2.809AlaPhe: 2.809 ± 0.364
7.092AlaGly: 7.092 ± 0.776
1.194AlaHis: 1.194 ± 0.261
5.758AlaIle: 5.758 ± 0.588
6.741AlaLys: 6.741 ± 0.664
8.707AlaLeu: 8.707 ± 0.921
3.651AlaMet: 3.651 ± 0.584
2.809AlaAsn: 2.809 ± 0.427
4.002AlaPro: 4.002 ± 0.67
4.704AlaGln: 4.704 ± 0.625
6.249AlaArg: 6.249 ± 0.772
6.46AlaSer: 6.46 ± 0.702
5.336AlaThr: 5.336 ± 0.539
5.687AlaVal: 5.687 ± 0.706
2.247AlaTrp: 2.247 ± 0.372
3.089AlaTyr: 3.089 ± 0.337
0.0AlaXaa: 0.0 ± 0.0
Cys
1.334CysAla: 1.334 ± 0.312
0.281CysCys: 0.281 ± 0.132
1.053CysAsp: 1.053 ± 0.278
0.913CysGlu: 0.913 ± 0.267
0.281CysPhe: 0.281 ± 0.121
1.264CysGly: 1.264 ± 0.296
0.281CysHis: 0.281 ± 0.148
0.702CysIle: 0.702 ± 0.195
0.281CysLys: 0.281 ± 0.133
1.264CysLeu: 1.264 ± 0.329
0.421CysMet: 0.421 ± 0.163
0.211CysAsn: 0.211 ± 0.126
0.913CysPro: 0.913 ± 0.257
0.562CysGln: 0.562 ± 0.226
1.194CysArg: 1.194 ± 0.253
0.983CysSer: 0.983 ± 0.31
0.702CysThr: 0.702 ± 0.252
0.702CysVal: 0.702 ± 0.174
0.632CysTrp: 0.632 ± 0.226
0.632CysTyr: 0.632 ± 0.194
0.0CysXaa: 0.0 ± 0.0
Asp
6.811AspAla: 6.811 ± 0.734
0.983AspCys: 0.983 ± 0.288
3.581AspAsp: 3.581 ± 0.56
3.932AspGlu: 3.932 ± 0.542
2.036AspPhe: 2.036 ± 0.332
5.196AspGly: 5.196 ± 0.62
0.983AspHis: 0.983 ± 0.314
2.738AspIle: 2.738 ± 0.44
2.247AspLys: 2.247 ± 0.348
4.915AspLeu: 4.915 ± 0.69
1.475AspMet: 1.475 ± 0.348
1.475AspAsn: 1.475 ± 0.376
3.23AspPro: 3.23 ± 0.477
1.966AspGln: 1.966 ± 0.285
3.581AspArg: 3.581 ± 0.496
3.511AspSer: 3.511 ± 0.495
2.738AspThr: 2.738 ± 0.534
3.862AspVal: 3.862 ± 0.395
1.123AspTrp: 1.123 ± 0.256
1.334AspTyr: 1.334 ± 0.36
0.0AspXaa: 0.0 ± 0.0
Glu
7.302GluAla: 7.302 ± 1.208
0.843GluCys: 0.843 ± 0.264
2.317GluAsp: 2.317 ± 0.421
3.721GluGlu: 3.721 ± 0.533
2.387GluPhe: 2.387 ± 0.299
3.3GluGly: 3.3 ± 0.509
1.685GluHis: 1.685 ± 0.261
3.441GluIle: 3.441 ± 0.459
3.37GluLys: 3.37 ± 0.57
6.951GluLeu: 6.951 ± 0.781
1.755GluMet: 1.755 ± 0.355
1.755GluAsn: 1.755 ± 0.319
3.089GluPro: 3.089 ± 0.574
5.336GluGln: 5.336 ± 0.832
7.092GluArg: 7.092 ± 0.885
3.441GluSer: 3.441 ± 0.5
2.598GluThr: 2.598 ± 0.431
4.002GluVal: 4.002 ± 0.576
1.685GluTrp: 1.685 ± 0.322
2.387GluTyr: 2.387 ± 0.441
0.0GluXaa: 0.0 ± 0.0
Phe
3.089PheAla: 3.089 ± 0.448
0.492PheCys: 0.492 ± 0.18
2.317PheAsp: 2.317 ± 0.422
2.738PheGlu: 2.738 ± 0.456
0.772PhePhe: 0.772 ± 0.235
2.809PheGly: 2.809 ± 0.505
0.913PheHis: 0.913 ± 0.271
1.826PheIle: 1.826 ± 0.376
1.615PheLys: 1.615 ± 0.348
1.896PheLeu: 1.896 ± 0.33
0.351PheMet: 0.351 ± 0.148
1.123PheAsn: 1.123 ± 0.223
1.404PhePro: 1.404 ± 0.296
1.053PheGln: 1.053 ± 0.268
2.177PheArg: 2.177 ± 0.335
2.598PheSer: 2.598 ± 0.414
2.247PheThr: 2.247 ± 0.426
2.036PheVal: 2.036 ± 0.386
0.281PheTrp: 0.281 ± 0.144
1.053PheTyr: 1.053 ± 0.212
0.0PheXaa: 0.0 ± 0.0
Gly
5.687GlyAla: 5.687 ± 0.528
1.194GlyCys: 1.194 ± 0.295
4.002GlyAsp: 4.002 ± 0.479
4.564GlyGlu: 4.564 ± 0.611
3.23GlyPhe: 3.23 ± 0.385
6.109GlyGly: 6.109 ± 0.632
1.404GlyHis: 1.404 ± 0.391
4.283GlyIle: 4.283 ± 0.589
3.441GlyLys: 3.441 ± 0.485
6.179GlyLeu: 6.179 ± 0.547
2.458GlyMet: 2.458 ± 0.363
2.106GlyAsn: 2.106 ± 0.412
2.106GlyPro: 2.106 ± 0.373
2.879GlyGln: 2.879 ± 0.43
3.721GlyArg: 3.721 ± 0.571
3.792GlySer: 3.792 ± 0.467
3.651GlyThr: 3.651 ± 0.69
6.038GlyVal: 6.038 ± 0.659
1.545GlyTrp: 1.545 ± 0.311
2.387GlyTyr: 2.387 ± 0.39
0.0GlyXaa: 0.0 ± 0.0
His
1.404HisAla: 1.404 ± 0.42
0.281HisCys: 0.281 ± 0.12
0.983HisAsp: 0.983 ± 0.193
1.053HisGlu: 1.053 ± 0.27
0.562HisPhe: 0.562 ± 0.224
1.334HisGly: 1.334 ± 0.351
0.562HisHis: 0.562 ± 0.233
0.843HisIle: 0.843 ± 0.225
1.053HisLys: 1.053 ± 0.298
1.545HisLeu: 1.545 ± 0.307
0.351HisMet: 0.351 ± 0.15
0.421HisAsn: 0.421 ± 0.145
1.264HisPro: 1.264 ± 0.292
0.772HisGln: 0.772 ± 0.287
1.615HisArg: 1.615 ± 0.301
0.913HisSer: 0.913 ± 0.2
0.281HisThr: 0.281 ± 0.142
1.194HisVal: 1.194 ± 0.297
0.281HisTrp: 0.281 ± 0.136
0.702HisTyr: 0.702 ± 0.261
0.0HisXaa: 0.0 ± 0.0
Ile
6.53IleAla: 6.53 ± 0.797
0.772IleCys: 0.772 ± 0.213
4.283IleAsp: 4.283 ± 0.514
3.721IleGlu: 3.721 ± 0.599
1.545IlePhe: 1.545 ± 0.302
4.634IleGly: 4.634 ± 0.583
0.632IleHis: 0.632 ± 0.201
2.458IleIle: 2.458 ± 0.484
2.387IleLys: 2.387 ± 0.395
3.932IleLeu: 3.932 ± 0.607
0.492IleMet: 0.492 ± 0.169
1.755IleAsn: 1.755 ± 0.35
2.879IlePro: 2.879 ± 0.43
2.177IleGln: 2.177 ± 0.303
3.581IleArg: 3.581 ± 0.42
3.37IleSer: 3.37 ± 0.37
3.23IleThr: 3.23 ± 0.433
3.019IleVal: 3.019 ± 0.513
1.194IleTrp: 1.194 ± 0.296
1.615IleTyr: 1.615 ± 0.34
0.0IleXaa: 0.0 ± 0.0
Lys
6.53LysAla: 6.53 ± 0.911
0.632LysCys: 0.632 ± 0.234
2.668LysAsp: 2.668 ± 0.539
3.019LysGlu: 3.019 ± 0.506
1.123LysPhe: 1.123 ± 0.269
3.792LysGly: 3.792 ± 0.553
0.983LysHis: 0.983 ± 0.249
1.755LysIle: 1.755 ± 0.322
1.826LysLys: 1.826 ± 0.324
3.721LysLeu: 3.721 ± 0.458
1.615LysMet: 1.615 ± 0.362
1.966LysAsn: 1.966 ± 0.337
2.387LysPro: 2.387 ± 0.413
1.545LysGln: 1.545 ± 0.368
3.932LysArg: 3.932 ± 0.527
3.721LysSer: 3.721 ± 0.482
2.668LysThr: 2.668 ± 0.522
4.072LysVal: 4.072 ± 0.434
0.702LysTrp: 0.702 ± 0.223
0.843LysTyr: 0.843 ± 0.234
0.0LysXaa: 0.0 ± 0.0
Leu
7.583LeuAla: 7.583 ± 0.816
1.194LeuCys: 1.194 ± 0.264
4.985LeuAsp: 4.985 ± 0.743
5.687LeuGlu: 5.687 ± 0.707
2.458LeuPhe: 2.458 ± 0.369
5.055LeuGly: 5.055 ± 0.519
0.772LeuHis: 0.772 ± 0.231
4.985LeuIle: 4.985 ± 0.458
4.775LeuLys: 4.775 ± 0.479
6.109LeuLeu: 6.109 ± 0.717
2.247LeuMet: 2.247 ± 0.446
3.792LeuAsn: 3.792 ± 0.687
4.424LeuPro: 4.424 ± 0.595
2.036LeuGln: 2.036 ± 0.322
6.53LeuArg: 6.53 ± 0.659
6.741LeuSer: 6.741 ± 0.74
3.792LeuThr: 3.792 ± 0.51
5.687LeuVal: 5.687 ± 0.644
0.702LeuTrp: 0.702 ± 0.229
2.317LeuTyr: 2.317 ± 0.379
0.0LeuXaa: 0.0 ± 0.0
Met
2.387MetAla: 2.387 ± 0.396
0.351MetCys: 0.351 ± 0.139
1.685MetAsp: 1.685 ± 0.294
1.334MetGlu: 1.334 ± 0.308
0.562MetPhe: 0.562 ± 0.192
1.053MetGly: 1.053 ± 0.28
0.421MetHis: 0.421 ± 0.164
1.194MetIle: 1.194 ± 0.321
1.334MetLys: 1.334 ± 0.362
2.317MetLeu: 2.317 ± 0.454
0.772MetMet: 0.772 ± 0.244
1.194MetAsn: 1.194 ± 0.301
1.755MetPro: 1.755 ± 0.393
1.404MetGln: 1.404 ± 0.281
2.036MetArg: 2.036 ± 0.403
2.387MetSer: 2.387 ± 0.4
2.036MetThr: 2.036 ± 0.357
1.264MetVal: 1.264 ± 0.312
0.07MetTrp: 0.07 ± 0.065
0.562MetTyr: 0.562 ± 0.193
0.0MetXaa: 0.0 ± 0.0
Asn
3.23AsnAla: 3.23 ± 0.556
0.702AsnCys: 0.702 ± 0.198
1.755AsnAsp: 1.755 ± 0.341
2.247AsnGlu: 2.247 ± 0.415
0.843AsnPhe: 0.843 ± 0.226
2.528AsnGly: 2.528 ± 0.494
0.843AsnHis: 0.843 ± 0.217
1.334AsnIle: 1.334 ± 0.402
1.194AsnLys: 1.194 ± 0.287
2.247AsnLeu: 2.247 ± 0.372
0.772AsnMet: 0.772 ± 0.238
1.264AsnAsn: 1.264 ± 0.271
2.387AsnPro: 2.387 ± 0.346
1.475AsnGln: 1.475 ± 0.371
1.826AsnArg: 1.826 ± 0.389
2.317AsnSer: 2.317 ± 0.455
1.685AsnThr: 1.685 ± 0.318
2.036AsnVal: 2.036 ± 0.44
0.492AsnTrp: 0.492 ± 0.168
1.053AsnTyr: 1.053 ± 0.303
0.0AsnXaa: 0.0 ± 0.0
Pro
5.898ProAla: 5.898 ± 0.729
0.843ProCys: 0.843 ± 0.249
3.3ProAsp: 3.3 ± 0.492
4.283ProGlu: 4.283 ± 0.561
2.387ProPhe: 2.387 ± 0.5
3.23ProGly: 3.23 ± 0.519
0.913ProHis: 0.913 ± 0.253
2.317ProIle: 2.317 ± 0.382
2.387ProLys: 2.387 ± 0.459
4.353ProLeu: 4.353 ± 0.612
1.123ProMet: 1.123 ± 0.306
1.404ProAsn: 1.404 ± 0.299
2.036ProPro: 2.036 ± 0.443
1.404ProGln: 1.404 ± 0.261
1.966ProArg: 1.966 ± 0.445
3.23ProSer: 3.23 ± 0.58
1.826ProThr: 1.826 ± 0.292
3.511ProVal: 3.511 ± 0.502
0.772ProTrp: 0.772 ± 0.221
1.123ProTyr: 1.123 ± 0.397
0.0ProXaa: 0.0 ± 0.0
Gln
6.179GlnAla: 6.179 ± 0.679
0.772GlnCys: 0.772 ± 0.219
1.264GlnAsp: 1.264 ± 0.269
2.738GlnGlu: 2.738 ± 0.475
1.615GlnPhe: 1.615 ± 0.324
2.809GlnGly: 2.809 ± 0.475
0.421GlnHis: 0.421 ± 0.155
2.458GlnIle: 2.458 ± 0.467
1.755GlnLys: 1.755 ± 0.291
3.37GlnLeu: 3.37 ± 0.658
1.053GlnMet: 1.053 ± 0.325
1.264GlnAsn: 1.264 ± 0.279
1.404GlnPro: 1.404 ± 0.271
2.106GlnGln: 2.106 ± 0.669
2.879GlnArg: 2.879 ± 0.631
2.106GlnSer: 2.106 ± 0.364
1.545GlnThr: 1.545 ± 0.34
2.738GlnVal: 2.738 ± 0.377
0.702GlnTrp: 0.702 ± 0.227
1.826GlnTyr: 1.826 ± 0.428
0.0GlnXaa: 0.0 ± 0.0
Arg
5.828ArgAla: 5.828 ± 0.692
0.702ArgCys: 0.702 ± 0.219
4.634ArgAsp: 4.634 ± 0.651
5.336ArgGlu: 5.336 ± 0.994
2.879ArgPhe: 2.879 ± 0.393
3.792ArgGly: 3.792 ± 0.553
1.475ArgHis: 1.475 ± 0.316
4.283ArgIle: 4.283 ± 0.504
3.792ArgLys: 3.792 ± 0.545
5.758ArgLeu: 5.758 ± 0.596
2.458ArgMet: 2.458 ± 0.412
1.685ArgAsn: 1.685 ± 0.321
2.949ArgPro: 2.949 ± 0.456
2.809ArgGln: 2.809 ± 0.595
5.055ArgArg: 5.055 ± 0.785
3.932ArgSer: 3.932 ± 0.546
2.879ArgThr: 2.879 ± 0.36
4.353ArgVal: 4.353 ± 0.528
1.123ArgTrp: 1.123 ± 0.291
2.317ArgTyr: 2.317 ± 0.464
0.0ArgXaa: 0.0 ± 0.0
Ser
7.092SerAla: 7.092 ± 0.779
0.562SerCys: 0.562 ± 0.212
4.143SerAsp: 4.143 ± 0.513
3.16SerGlu: 3.16 ± 0.498
2.106SerPhe: 2.106 ± 0.434
6.811SerGly: 6.811 ± 0.916
0.983SerHis: 0.983 ± 0.285
4.002SerIle: 4.002 ± 0.564
2.949SerLys: 2.949 ± 0.474
5.266SerLeu: 5.266 ± 0.793
1.615SerMet: 1.615 ± 0.311
2.387SerAsn: 2.387 ± 0.534
2.668SerPro: 2.668 ± 0.394
1.966SerGln: 1.966 ± 0.325
3.511SerArg: 3.511 ± 0.591
4.072SerSer: 4.072 ± 0.625
3.511SerThr: 3.511 ± 0.505
4.494SerVal: 4.494 ± 0.646
1.194SerTrp: 1.194 ± 0.271
2.247SerTyr: 2.247 ± 0.558
0.0SerXaa: 0.0 ± 0.0
Thr
5.617ThrAla: 5.617 ± 0.687
0.983ThrCys: 0.983 ± 0.228
2.668ThrAsp: 2.668 ± 0.413
3.23ThrGlu: 3.23 ± 0.449
1.826ThrPhe: 1.826 ± 0.403
2.317ThrGly: 2.317 ± 0.398
0.843ThrHis: 0.843 ± 0.247
3.23ThrIle: 3.23 ± 0.454
2.528ThrLys: 2.528 ± 0.481
4.283ThrLeu: 4.283 ± 0.524
1.123ThrMet: 1.123 ± 0.237
1.545ThrAsn: 1.545 ± 0.267
2.949ThrPro: 2.949 ± 0.44
2.317ThrGln: 2.317 ± 0.43
2.598ThrArg: 2.598 ± 0.459
3.089ThrSer: 3.089 ± 0.787
3.441ThrThr: 3.441 ± 0.601
4.143ThrVal: 4.143 ± 0.484
0.772ThrTrp: 0.772 ± 0.216
1.264ThrTyr: 1.264 ± 0.303
0.0ThrXaa: 0.0 ± 0.0
Val
6.53ValAla: 6.53 ± 0.626
0.983ValCys: 0.983 ± 0.272
3.37ValAsp: 3.37 ± 0.533
4.775ValGlu: 4.775 ± 0.546
1.755ValPhe: 1.755 ± 0.372
4.775ValGly: 4.775 ± 0.455
0.983ValHis: 0.983 ± 0.209
4.002ValIle: 4.002 ± 0.464
3.721ValLys: 3.721 ± 0.523
4.845ValLeu: 4.845 ± 0.698
0.913ValMet: 0.913 ± 0.256
2.738ValAsn: 2.738 ± 0.39
3.932ValPro: 3.932 ± 0.514
2.247ValGln: 2.247 ± 0.377
4.494ValArg: 4.494 ± 0.564
4.704ValSer: 4.704 ± 0.614
4.424ValThr: 4.424 ± 0.566
3.932ValVal: 3.932 ± 0.455
1.194ValTrp: 1.194 ± 0.232
2.036ValTyr: 2.036 ± 0.304
0.0ValXaa: 0.0 ± 0.0
Trp
1.123TrpAla: 1.123 ± 0.281
0.211TrpCys: 0.211 ± 0.127
0.772TrpAsp: 0.772 ± 0.234
1.053TrpGlu: 1.053 ± 0.23
0.562TrpPhe: 0.562 ± 0.202
0.632TrpGly: 0.632 ± 0.238
0.492TrpHis: 0.492 ± 0.19
1.264TrpIle: 1.264 ± 0.315
0.913TrpLys: 0.913 ± 0.272
1.966TrpLeu: 1.966 ± 0.346
0.492TrpMet: 0.492 ± 0.175
0.421TrpAsn: 0.421 ± 0.159
0.983TrpPro: 0.983 ± 0.259
1.194TrpGln: 1.194 ± 0.329
1.615TrpArg: 1.615 ± 0.296
1.475TrpSer: 1.475 ± 0.303
0.702TrpThr: 0.702 ± 0.22
1.545TrpVal: 1.545 ± 0.283
0.421TrpTrp: 0.421 ± 0.178
0.281TrpTyr: 0.281 ± 0.112
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.177TyrAla: 2.177 ± 0.337
0.421TyrCys: 0.421 ± 0.167
2.387TyrAsp: 2.387 ± 0.434
2.106TyrGlu: 2.106 ± 0.38
1.053TyrPhe: 1.053 ± 0.271
2.247TyrGly: 2.247 ± 0.392
0.632TyrHis: 0.632 ± 0.177
1.334TyrIle: 1.334 ± 0.249
1.194TyrLys: 1.194 ± 0.225
2.247TyrLeu: 2.247 ± 0.399
0.913TyrMet: 0.913 ± 0.221
0.913TyrAsn: 0.913 ± 0.309
1.545TyrPro: 1.545 ± 0.488
1.053TyrGln: 1.053 ± 0.244
2.387TyrArg: 2.387 ± 0.526
1.966TyrSer: 1.966 ± 0.419
1.475TyrThr: 1.475 ± 0.459
2.036TyrVal: 2.036 ± 0.493
0.983TyrTrp: 0.983 ± 0.249
0.843TyrTyr: 0.843 ± 0.22
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (14243 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski