Nitratiruptor phage NrS-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M5AAF3|M5AAF3_9CAUD Uncharacterized protein OS=Nitratiruptor phage NrS-1 OX=1230469 PE=4 SV=1
MM1 pKa = 7.22AQIIQHH7 pKa = 6.12RR8 pKa = 11.84RR9 pKa = 11.84DD10 pKa = 3.34TKK12 pKa = 11.43ANWEE16 pKa = 4.09QYY18 pKa = 11.21DD19 pKa = 3.71PVLAAGEE26 pKa = 4.41VAVQTDD32 pKa = 3.86TYY34 pKa = 9.86QIKK37 pKa = 10.91VGDD40 pKa = 4.14GVKK43 pKa = 9.81KK44 pKa = 9.36WSEE47 pKa = 3.88LPFVSFGLLDD57 pKa = 3.68NPEE60 pKa = 4.69GYY62 pKa = 10.57FEE64 pKa = 4.31TLSITGVVYY73 pKa = 10.48DD74 pKa = 4.65ANNMPTEE81 pKa = 4.17ITFSNGSKK89 pKa = 10.74ALYY92 pKa = 9.49TYY94 pKa = 10.93DD95 pKa = 3.78AATGLLTEE103 pKa = 4.21TDD105 pKa = 3.66YY106 pKa = 11.49TKK108 pKa = 11.21EE109 pKa = 3.99DD110 pKa = 3.66GTTVFYY116 pKa = 10.71KK117 pKa = 10.49VQYY120 pKa = 10.4TYY122 pKa = 10.01DD123 pKa = 3.76TNNLLTSVTRR133 pKa = 11.84SYY135 pKa = 11.2VV136 pKa = 3.03

Molecular weight: 15.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M5A9D8|M5A9D8_9CAUD Uncharacterized protein OS=Nitratiruptor phage NrS-1 OX=1230469 PE=4 SV=1
MM1 pKa = 7.48SGYY4 pKa = 8.71TFYY7 pKa = 10.94TLRR10 pKa = 11.84LHH12 pKa = 6.36FWKK15 pKa = 10.5SYY17 pKa = 6.92TLKK20 pKa = 10.87CNPTKK25 pKa = 10.48RR26 pKa = 11.84KK27 pKa = 10.4SIFNQFYY34 pKa = 7.88LTKK37 pKa = 8.76WLHH40 pKa = 6.1FEE42 pKa = 4.03KK43 pKa = 10.78CNPKK47 pKa = 10.28CNQKK51 pKa = 10.35CNHH54 pKa = 6.53RR55 pKa = 11.84EE56 pKa = 3.84CLQNGSFNTTVTLVTLFFNKK76 pKa = 9.98FSKK79 pKa = 9.87TLFIKK84 pKa = 10.52RR85 pKa = 11.84KK86 pKa = 6.72EE87 pKa = 4.0EE88 pKa = 3.82KK89 pKa = 10.26VEE91 pKa = 4.15KK92 pKa = 10.52KK93 pKa = 10.18RR94 pKa = 11.84GFYY97 pKa = 10.48NIYY100 pKa = 9.58KK101 pKa = 10.03SVVKK105 pKa = 10.53SVTLRR110 pKa = 11.84FEE112 pKa = 4.08NRR114 pKa = 11.84IKK116 pKa = 10.9RR117 pKa = 11.84LLRR120 pKa = 11.84NINRR124 pKa = 11.84KK125 pKa = 7.38NQSVVIGVV133 pKa = 3.46

Molecular weight: 16.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11116

56

1421

218.0

24.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.476 ± 0.598

0.846 ± 0.16

6.135 ± 0.218

7.845 ± 0.468

4.291 ± 0.212

5.766 ± 0.335

1.556 ± 0.201

7.404 ± 0.27

9.635 ± 0.47

8.159 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.146

4.903 ± 0.187

3.005 ± 0.225

3.427 ± 0.267

4.021 ± 0.287

5.973 ± 0.323

5.847 ± 0.376

5.955 ± 0.329

1.313 ± 0.144

4.336 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski