Clostridium sp. CAG:492

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1098 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5YWH8|R5YWH8_9CLOT Aldose 1-epimerase OS=Clostridium sp. CAG:492 OX=1262813 GN=BN681_00391 PE=4 SV=1
MM1 pKa = 7.86ILQDD5 pKa = 4.44YY6 pKa = 11.07LNDD9 pKa = 3.85DD10 pKa = 3.59QVNLCEE16 pKa = 3.97EE17 pKa = 4.05TGIIIEE23 pKa = 4.54DD24 pKa = 3.3RR25 pKa = 11.84DD26 pKa = 3.91YY27 pKa = 11.46SCEE30 pKa = 3.75EE31 pKa = 3.92IYY33 pKa = 10.78QIEE36 pKa = 4.43HH37 pKa = 7.43KK38 pKa = 10.67IMEE41 pKa = 4.37YY42 pKa = 10.42MNDD45 pKa = 2.99NCADD49 pKa = 3.55EE50 pKa = 5.47NIYY53 pKa = 10.81LLEE56 pKa = 4.26EE57 pKa = 4.67KK58 pKa = 10.44YY59 pKa = 11.19DD60 pKa = 4.28EE61 pKa = 4.61ILDD64 pKa = 3.58ILMDD68 pKa = 4.42LEE70 pKa = 4.51NEE72 pKa = 4.02SDD74 pKa = 4.37EE75 pKa = 4.32INPMIVEE82 pKa = 4.19INEE85 pKa = 4.3DD86 pKa = 3.46DD87 pKa = 4.47HH88 pKa = 10.05VEE90 pKa = 4.06LNNGKK95 pKa = 9.94SGIVADD101 pKa = 3.81ITNNVYY107 pKa = 10.0TIEE110 pKa = 3.95VDD112 pKa = 3.78EE113 pKa = 4.62KK114 pKa = 11.02FKK116 pKa = 10.75TGNIDD121 pKa = 3.52DD122 pKa = 5.87DD123 pKa = 4.55IMIVAANSIIGRR135 pKa = 11.84KK136 pKa = 8.45

Molecular weight: 15.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5ZNP7|R5ZNP7_9CLOT Acetate kinase OS=Clostridium sp. CAG:492 OX=1262813 GN=ackA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.57RR3 pKa = 11.84TFQPKK8 pKa = 6.98TRR10 pKa = 11.84QMKK13 pKa = 10.04KK14 pKa = 7.31EE15 pKa = 3.86HH16 pKa = 6.14GFMKK20 pKa = 10.36RR21 pKa = 11.84MKK23 pKa = 8.99TRR25 pKa = 11.84SGRR28 pKa = 11.84NVLKK32 pKa = 10.63ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.37GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1098

0

1098

324855

29

4092

295.9

33.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.206 ± 0.082

1.198 ± 0.03

5.731 ± 0.065

7.9 ± 0.098

3.849 ± 0.054

5.786 ± 0.076

1.191 ± 0.028

10.217 ± 0.095

10.168 ± 0.089

8.157 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.43 ± 0.042

7.309 ± 0.099

2.558 ± 0.041

2.701 ± 0.034

3.081 ± 0.052

5.972 ± 0.073

5.397 ± 0.074

6.165 ± 0.078

0.615 ± 0.024

4.369 ± 0.071
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski