Nitrospira japonica

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Nitrospira

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4147 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W1I2E4|A0A1W1I2E4_9BACT Putative DNA polymerase III delta subunit OS=Nitrospira japonica OX=1325564 GN=holA PE=4 SV=1
MM1 pKa = 7.26LTLIHH6 pKa = 6.79RR7 pKa = 11.84EE8 pKa = 4.11CGGPALEE15 pKa = 4.56TNTAVGEE22 pKa = 4.45VCALPVDD29 pKa = 4.58RR30 pKa = 11.84FPFPCFTCLEE40 pKa = 5.04DD41 pKa = 4.62ILDD44 pKa = 3.93EE45 pKa = 4.66SEE47 pKa = 4.11VRR49 pKa = 11.84LSEE52 pKa = 4.03EE53 pKa = 4.26LGII56 pKa = 4.83

Molecular weight: 6.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W1I2N3|A0A1W1I2N3_9BACT Putative Sulfite:cytochrome c oxidoreductase subunit A OS=Nitrospira japonica OX=1325564 GN=NSJP_1095 PE=4 SV=1
MM1 pKa = 6.98KK2 pKa = 10.1QARR5 pKa = 11.84TANRR9 pKa = 11.84LHH11 pKa = 6.47RR12 pKa = 11.84VQRR15 pKa = 11.84VVRR18 pKa = 11.84LLVLGAAVLCFASAQPASGGAGPGSTEE45 pKa = 3.67RR46 pKa = 11.84AAGQAQAGKK55 pKa = 10.46AVFNGKK61 pKa = 8.35GVCFYY66 pKa = 11.14CHH68 pKa = 6.2GVDD71 pKa = 4.01GRR73 pKa = 11.84RR74 pKa = 11.84DD75 pKa = 3.34RR76 pKa = 11.84LPRR79 pKa = 11.84IEE81 pKa = 5.61ADD83 pKa = 3.33TAALIAQLNPPPADD97 pKa = 3.11LRR99 pKa = 11.84VPSRR103 pKa = 11.84LRR105 pKa = 11.84LKK107 pKa = 9.71TDD109 pKa = 3.4RR110 pKa = 11.84EE111 pKa = 4.07RR112 pKa = 11.84TSAIRR117 pKa = 11.84NGHH120 pKa = 6.99PGTGMFPDD128 pKa = 3.87TRR130 pKa = 11.84ITDD133 pKa = 3.42QEE135 pKa = 4.25LADD138 pKa = 3.87TLAYY142 pKa = 10.39LAVLRR147 pKa = 11.84QEE149 pKa = 4.47GTPTPP154 pKa = 3.94

Molecular weight: 16.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4147

0

4147

1241337

20

4422

299.3

32.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.826 ± 0.043

1.013 ± 0.013

5.29 ± 0.024

5.856 ± 0.04

3.685 ± 0.025

7.973 ± 0.042

2.362 ± 0.018

5.022 ± 0.029

3.851 ± 0.037

10.393 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.378 ± 0.018

2.686 ± 0.033

5.279 ± 0.03

3.818 ± 0.032

7.243 ± 0.042

6.038 ± 0.032

5.643 ± 0.047

7.668 ± 0.033

1.4 ± 0.019

2.577 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski