Clostridium virus phiCD27

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Lubbockvirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6SBY6|B6SBY6_9CAUD Putative DNA methylase OS=Clostridium virus phiCD27 OX=559189 PE=3 SV=1
MM1 pKa = 7.6NILASVILVIGSFIAGRR18 pKa = 11.84VYY20 pKa = 10.41EE21 pKa = 4.06YY22 pKa = 11.07RR23 pKa = 11.84LNLNEE28 pKa = 4.44NDD30 pKa = 3.75EE31 pKa = 4.63ADD33 pKa = 3.96SKK35 pKa = 11.82VLLDD39 pKa = 3.6VFNEE43 pKa = 3.73ISVLRR48 pKa = 11.84EE49 pKa = 3.62EE50 pKa = 4.33NKK52 pKa = 10.3SLKK55 pKa = 10.39EE56 pKa = 3.77KK57 pKa = 10.45LQEE60 pKa = 4.56KK61 pKa = 9.99EE62 pKa = 3.87LLFINRR68 pKa = 11.84LIDD71 pKa = 3.61FLHH74 pKa = 6.94DD75 pKa = 4.16KK76 pKa = 10.31KK77 pKa = 10.89ICEE80 pKa = 4.18CCIYY84 pKa = 10.44DD85 pKa = 4.99CKK87 pKa = 10.64IDD89 pKa = 4.92DD90 pKa = 4.51IEE92 pKa = 4.36YY93 pKa = 10.35DD94 pKa = 3.9CEE96 pKa = 4.77DD97 pKa = 5.07GIKK100 pKa = 10.01KK101 pKa = 9.48WLDD104 pKa = 3.18SEE106 pKa = 4.5EE107 pKa = 5.24LIFEE111 pKa = 4.33

Molecular weight: 13.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6SBZ9|B6SBZ9_9CAUD Bro-N domain-containing protein OS=Clostridium virus phiCD27 OX=559189 PE=4 SV=1
MM1 pKa = 7.22YY2 pKa = 9.29PPLKK6 pKa = 10.21RR7 pKa = 11.84LRR9 pKa = 11.84LTSRR13 pKa = 11.84SFLIQKK19 pKa = 8.22FIKK22 pKa = 9.76NARR25 pKa = 11.84HH26 pKa = 5.13ALFRR30 pKa = 11.84YY31 pKa = 9.34ACHH34 pKa = 6.78ACLKK38 pKa = 10.92SNII41 pKa = 3.98

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

15399

37

1429

205.3

23.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.254 ± 0.38

1.13 ± 0.166

5.565 ± 0.225

8.721 ± 0.406

3.981 ± 0.178

5.819 ± 0.497

1.039 ± 0.138

8.91 ± 0.314

10.501 ± 0.362

8.488 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.112

6.961 ± 0.25

2.065 ± 0.123

2.981 ± 0.176

3.598 ± 0.209

6.156 ± 0.342

5.767 ± 0.216

5.806 ± 0.251

0.922 ± 0.085

4.065 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski