Thermocrinis albus (strain DSM 14484 / JCM 11386 / HI 11/12)

Taxonomy: cellular organisms; Bacteria; Aquificae; Aquificae; Aquificales; Aquificaceae; Thermocrinis; Thermocrinis albus

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1592 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|D3SPK9|D3SPK9_THEAH Flavocytochrome c sulphide dehydrogenase flavin-binding protein OS=Thermocrinis albus (strain DSM 14484 / JCM 11386 / HI 11/12) OX=638303 GN=Thal_0462 PE=4 SV=1
MM1 pKa = 7.88PYY3 pKa = 10.16SIKK6 pKa = 10.31KK7 pKa = 9.57DD8 pKa = 3.55LCISCYY14 pKa = 10.46ACEE17 pKa = 4.29DD18 pKa = 3.47QCPTKK23 pKa = 10.54AISHH27 pKa = 5.61NEE29 pKa = 3.66EE30 pKa = 3.99GEE32 pKa = 4.14FFIDD36 pKa = 3.2PTVCVEE42 pKa = 4.18CEE44 pKa = 3.89GFYY47 pKa = 11.0EE48 pKa = 4.7EE49 pKa = 4.65PQCLAICPIDD59 pKa = 3.61GCIVKK64 pKa = 10.44LSEE67 pKa = 4.08VEE69 pKa = 4.28EE70 pKa = 4.42VV71 pKa = 3.14

Molecular weight:
7.96 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|D3SM72|D3SM72_THEAH Beta-lactamase domain protein OS=Thermocrinis albus (strain DSM 14484 / JCM 11386 / HI 11/12) OX=638303 GN=Thal_1220 PE=4 SV=1
MM1 pKa = 6.63ATKK4 pKa = 10.49RR5 pKa = 11.84NVTHH9 pKa = 7.2ISNLKK14 pKa = 9.63RR15 pKa = 11.84KK16 pKa = 9.29RR17 pKa = 11.84KK18 pKa = 9.7SGFLARR24 pKa = 11.84MSSKK28 pKa = 10.36SGRR31 pKa = 11.84KK32 pKa = 8.27IIKK35 pKa = 9.47RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.52KK40 pKa = 10.18GRR42 pKa = 11.84HH43 pKa = 4.83RR44 pKa = 11.84LAPP47 pKa = 3.97

Molecular weight:
5.54 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1592

0

1592

479659

30

1566

301.3

34.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.307 ± 0.058

0.94 ± 0.025

4.775 ± 0.035

7.991 ± 0.075

4.377 ± 0.049

7.208 ± 0.056

1.926 ± 0.03

6.174 ± 0.051

6.695 ± 0.058

11.466 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.027

2.816 ± 0.031

4.36 ± 0.037

2.802 ± 0.036

6.174 ± 0.05

5.367 ± 0.047

4.433 ± 0.032

8.687 ± 0.064

1.131 ± 0.025

3.97 ± 0.041

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski