Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces davaonensis

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4R718|K4R718_STRDJ Glyco_trans_2-like domain-containing protein OS=Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768) OX=1214101 GN=BN159_4513 PE=4 SV=1
MM1 pKa = 7.06TQFPTLIDD9 pKa = 3.43QVAAAVLAPAQVTEE23 pKa = 4.46NPHH26 pKa = 5.34HH27 pKa = 6.64RR28 pKa = 11.84AILDD32 pKa = 3.6VFVEE36 pKa = 4.13IQDD39 pKa = 4.29LDD41 pKa = 3.65RR42 pKa = 11.84QLAEE46 pKa = 3.99LAGAEE51 pKa = 4.17LPGVSPAEE59 pKa = 3.96QLNTLTDD66 pKa = 4.07LLVEE70 pKa = 4.57RR71 pKa = 11.84MAAGADD77 pKa = 3.88LSALLATSCCCAAAYY92 pKa = 7.8EE93 pKa = 4.54AGPSAEE99 pKa = 4.45YY100 pKa = 10.71EE101 pKa = 4.12PLDD104 pKa = 4.28DD105 pKa = 4.93EE106 pKa = 5.45EE107 pKa = 6.04PGFDD111 pKa = 4.26PDD113 pKa = 4.33FDD115 pKa = 4.35DD116 pKa = 6.84FEE118 pKa = 6.15GFDD121 pKa = 4.2DD122 pKa = 5.03FEE124 pKa = 4.9EE125 pKa = 5.29PGDD128 pKa = 4.73LDD130 pKa = 5.77DD131 pKa = 7.1FDD133 pKa = 5.92DD134 pKa = 4.09LHH136 pKa = 7.37EE137 pKa = 4.8DD138 pKa = 3.14ACEE141 pKa = 3.97EE142 pKa = 4.15FLSCPSGPQTIVHH155 pKa = 6.7ACLAVFDD162 pKa = 5.31DD163 pKa = 4.67EE164 pKa = 4.85GAPEE168 pKa = 4.07YY169 pKa = 11.1VSAAEE174 pKa = 3.87LVEE177 pKa = 4.13RR178 pKa = 11.84LRR180 pKa = 11.84DD181 pKa = 3.55LPGQAEE187 pKa = 4.54GRR189 pKa = 11.84WSYY192 pKa = 12.24ADD194 pKa = 3.69LTPLRR199 pKa = 11.84LGLLLRR205 pKa = 11.84GYY207 pKa = 10.08GVQPCNPRR215 pKa = 11.84AADD218 pKa = 3.76GSRR221 pKa = 11.84YY222 pKa = 8.93RR223 pKa = 11.84AYY225 pKa = 10.62RR226 pKa = 11.84RR227 pKa = 11.84ADD229 pKa = 3.55LLAARR234 pKa = 11.84PNCSCC239 pKa = 4.98

Molecular weight: 25.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4R799|K4R799_STRDJ Integral membrane protein OS=Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768) OX=1214101 GN=BN159_4849 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.46GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8553

0

8553

2841216

28

6221

332.2

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.269 ± 0.041

0.797 ± 0.007

6.063 ± 0.021

5.88 ± 0.022

2.742 ± 0.016

9.195 ± 0.023

2.338 ± 0.012

3.192 ± 0.02

2.204 ± 0.023

10.466 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.715 ± 0.012

1.826 ± 0.015

6.07 ± 0.027

2.829 ± 0.015

7.982 ± 0.032

5.101 ± 0.019

6.237 ± 0.021

8.361 ± 0.027

1.585 ± 0.011

2.148 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski