Photinus pyralis (Common eastern firefly) (Lampyris pyralis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Elateriformia; Elateroidea; Lampyridae;

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14952 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y1LW23|A0A1Y1LW23_PHOPY TPR_REGION domain-containing protein OS=Photinus pyralis OX=7054 GN=PPYR_12191 PE=4 SV=1
MM1 pKa = 6.57EE2 pKa = 4.65TVVATPTTVYY12 pKa = 10.53EE13 pKa = 4.04QIQNIFYY20 pKa = 10.06AIPILITIHH29 pKa = 5.73FLFYY33 pKa = 10.96AFVIAAYY40 pKa = 7.57NTSNGEE46 pKa = 4.1FFHH49 pKa = 8.35SLINAQEE56 pKa = 4.15EE57 pKa = 4.54KK58 pKa = 11.11EE59 pKa = 4.1EE60 pKa = 4.06VLVIEE65 pKa = 4.6ARR67 pKa = 11.84KK68 pKa = 9.92QYY70 pKa = 10.99VIAKK74 pKa = 9.18WDD76 pKa = 3.8DD77 pKa = 3.39QSDD80 pKa = 3.69EE81 pKa = 5.57DD82 pKa = 6.18DD83 pKa = 5.79DD84 pKa = 6.97DD85 pKa = 6.43DD86 pKa = 4.61STWDD90 pKa = 3.3KK91 pKa = 11.68CEE93 pKa = 3.78EE94 pKa = 4.11CLGNSSSPSDD104 pKa = 3.65TPPP107 pKa = 3.86

Molecular weight: 12.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N4B4P7|A0A5N4B4P7_PHOPY PlsC domain-containing protein OS=Photinus pyralis OX=7054 GN=PPYR_01300 PE=3 SV=1
MM1 pKa = 7.41SSNRR5 pKa = 11.84FLRR8 pKa = 11.84VQGSAAIEE16 pKa = 4.0YY17 pKa = 10.63EE18 pKa = 4.26PDD20 pKa = 3.08QKK22 pKa = 11.46SCFRR26 pKa = 11.84MRR28 pKa = 11.84GEE30 pKa = 3.89YY31 pKa = 10.62LKK33 pKa = 10.92FPILKK38 pKa = 10.05SILRR42 pKa = 11.84ILSARR47 pKa = 11.84VSSLGRR53 pKa = 11.84LEE55 pKa = 4.71CFGLRR60 pKa = 11.84VLSLSVLDD68 pKa = 3.75QFVFPLHH75 pKa = 5.03LTEE78 pKa = 4.76RR79 pKa = 11.84VLFFKK84 pKa = 11.02LNNRR88 pKa = 11.84LDD90 pKa = 3.99FGNLICNSKK99 pKa = 9.86GRR101 pKa = 11.84RR102 pKa = 11.84ILSRR106 pKa = 11.84ISGFPLYY113 pKa = 10.34QQ114 pKa = 4.35

Molecular weight: 13.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14951

1

14952

7462682

50

18737

499.1

56.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.771 ± 0.018

2.139 ± 0.026

5.384 ± 0.015

6.48 ± 0.032

4.121 ± 0.019

5.29 ± 0.027

2.538 ± 0.009

6.129 ± 0.016

6.667 ± 0.029

9.51 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.206 ± 0.009

5.311 ± 0.017

4.878 ± 0.027

4.028 ± 0.015

5.064 ± 0.016

7.814 ± 0.023

5.797 ± 0.017

6.388 ± 0.018

1.097 ± 0.007

3.388 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski