Amino acid dipepetide frequency for Arthrobacter phage Wilde

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.094AlaAla: 14.094 ± 2.001
0.611AlaCys: 0.611 ± 0.196
7.235AlaAsp: 7.235 ± 0.761
7.329AlaGlu: 7.329 ± 0.708
3.336AlaPhe: 3.336 ± 0.539
8.362AlaGly: 8.362 ± 0.754
1.644AlaHis: 1.644 ± 0.332
4.745AlaIle: 4.745 ± 0.512
5.544AlaLys: 5.544 ± 0.609
8.832AlaLeu: 8.832 ± 0.706
3.383AlaMet: 3.383 ± 0.398
4.416AlaAsn: 4.416 ± 0.877
4.463AlaPro: 4.463 ± 0.561
3.57AlaGln: 3.57 ± 0.509
4.463AlaArg: 4.463 ± 0.464
6.107AlaSer: 6.107 ± 0.597
5.966AlaThr: 5.966 ± 0.736
5.544AlaVal: 5.544 ± 0.515
1.879AlaTrp: 1.879 ± 0.301
1.926AlaTyr: 1.926 ± 0.26
0.0AlaXaa: 0.0 ± 0.0
Cys
0.658CysAla: 0.658 ± 0.184
0.047CysCys: 0.047 ± 0.047
0.705CysAsp: 0.705 ± 0.216
0.329CysGlu: 0.329 ± 0.137
0.094CysPhe: 0.094 ± 0.066
0.94CysGly: 0.94 ± 0.314
0.235CysHis: 0.235 ± 0.096
0.282CysIle: 0.282 ± 0.133
0.423CysLys: 0.423 ± 0.156
0.517CysLeu: 0.517 ± 0.158
0.188CysMet: 0.188 ± 0.097
0.188CysAsn: 0.188 ± 0.091
0.423CysPro: 0.423 ± 0.184
0.376CysGln: 0.376 ± 0.147
0.846CysArg: 0.846 ± 0.232
0.846CysSer: 0.846 ± 0.243
0.611CysThr: 0.611 ± 0.195
0.47CysVal: 0.47 ± 0.131
0.188CysTrp: 0.188 ± 0.1
0.282CysTyr: 0.282 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
7.517AspAla: 7.517 ± 0.43
0.658AspCys: 0.658 ± 0.252
4.886AspAsp: 4.886 ± 0.513
4.651AspGlu: 4.651 ± 0.56
2.49AspPhe: 2.49 ± 0.424
6.812AspGly: 6.812 ± 0.692
1.409AspHis: 1.409 ± 0.316
2.725AspIle: 2.725 ± 0.345
3.054AspLys: 3.054 ± 0.368
4.933AspLeu: 4.933 ± 0.45
1.503AspMet: 1.503 ± 0.27
2.678AspAsn: 2.678 ± 0.364
4.134AspPro: 4.134 ± 0.539
2.349AspGln: 2.349 ± 0.352
2.96AspArg: 2.96 ± 0.379
3.711AspSer: 3.711 ± 0.479
3.195AspThr: 3.195 ± 0.32
3.711AspVal: 3.711 ± 0.363
1.55AspTrp: 1.55 ± 0.237
2.114AspTyr: 2.114 ± 0.335
0.0AspXaa: 0.0 ± 0.0
Glu
7.658GluAla: 7.658 ± 0.821
0.705GluCys: 0.705 ± 0.202
4.134GluAsp: 4.134 ± 0.686
5.027GluGlu: 5.027 ± 0.761
2.443GluPhe: 2.443 ± 0.421
5.919GluGly: 5.919 ± 0.719
1.644GluHis: 1.644 ± 0.266
2.725GluIle: 2.725 ± 0.447
3.429GluLys: 3.429 ± 0.5
6.06GluLeu: 6.06 ± 0.636
2.02GluMet: 2.02 ± 0.288
1.832GluAsn: 1.832 ± 0.468
2.772GluPro: 2.772 ± 0.476
2.349GluGln: 2.349 ± 0.306
5.121GluArg: 5.121 ± 0.61
4.087GluSer: 4.087 ± 0.517
3.148GluThr: 3.148 ± 0.333
5.356GluVal: 5.356 ± 0.726
1.362GluTrp: 1.362 ± 0.265
1.503GluTyr: 1.503 ± 0.369
0.0GluXaa: 0.0 ± 0.0
Phe
3.101PheAla: 3.101 ± 0.379
0.423PheCys: 0.423 ± 0.144
2.772PheAsp: 2.772 ± 0.371
2.349PheGlu: 2.349 ± 0.305
0.94PhePhe: 0.94 ± 0.227
3.007PheGly: 3.007 ± 0.329
0.47PheHis: 0.47 ± 0.199
2.067PheIle: 2.067 ± 0.405
1.597PheLys: 1.597 ± 0.256
1.973PheLeu: 1.973 ± 0.296
0.94PheMet: 0.94 ± 0.229
1.174PheAsn: 1.174 ± 0.288
1.034PhePro: 1.034 ± 0.24
0.987PheGln: 0.987 ± 0.203
1.738PheArg: 1.738 ± 0.241
1.785PheSer: 1.785 ± 0.249
2.866PheThr: 2.866 ± 0.289
1.926PheVal: 1.926 ± 0.291
0.658PheTrp: 0.658 ± 0.309
1.174PheTyr: 1.174 ± 0.328
0.0PheXaa: 0.0 ± 0.0
Gly
5.825GlyAla: 5.825 ± 0.632
0.611GlyCys: 0.611 ± 0.219
4.416GlyAsp: 4.416 ± 0.482
6.107GlyGlu: 6.107 ± 0.607
3.617GlyPhe: 3.617 ± 0.481
6.812GlyGly: 6.812 ± 0.929
2.302GlyHis: 2.302 ± 0.41
4.98GlyIle: 4.98 ± 0.654
4.087GlyLys: 4.087 ± 0.459
6.107GlyLeu: 6.107 ± 0.666
2.631GlyMet: 2.631 ± 0.423
3.429GlyAsn: 3.429 ± 0.708
3.758GlyPro: 3.758 ± 0.438
2.772GlyGln: 2.772 ± 0.449
4.416GlyArg: 4.416 ± 0.498
4.604GlySer: 4.604 ± 0.547
5.919GlyThr: 5.919 ± 0.816
4.745GlyVal: 4.745 ± 0.489
2.349GlyTrp: 2.349 ± 0.331
2.537GlyTyr: 2.537 ± 0.295
0.0GlyXaa: 0.0 ± 0.0
His
2.208HisAla: 2.208 ± 0.396
0.235HisCys: 0.235 ± 0.106
1.034HisAsp: 1.034 ± 0.306
1.034HisGlu: 1.034 ± 0.299
1.034HisPhe: 1.034 ± 0.336
1.597HisGly: 1.597 ± 0.228
0.846HisHis: 0.846 ± 0.235
1.174HisIle: 1.174 ± 0.22
0.94HisLys: 0.94 ± 0.215
1.879HisLeu: 1.879 ± 0.293
0.47HisMet: 0.47 ± 0.156
0.329HisAsn: 0.329 ± 0.125
1.879HisPro: 1.879 ± 0.401
0.705HisGln: 0.705 ± 0.153
1.174HisArg: 1.174 ± 0.331
1.081HisSer: 1.081 ± 0.284
0.799HisThr: 0.799 ± 0.182
1.456HisVal: 1.456 ± 0.308
0.517HisTrp: 0.517 ± 0.138
0.799HisTyr: 0.799 ± 0.212
0.0HisXaa: 0.0 ± 0.0
Ile
4.98IleAla: 4.98 ± 0.534
0.188IleCys: 0.188 ± 0.091
4.228IleAsp: 4.228 ± 0.418
3.899IleGlu: 3.899 ± 0.406
1.081IlePhe: 1.081 ± 0.253
3.711IleGly: 3.711 ± 0.488
0.846IleHis: 0.846 ± 0.264
2.208IleIle: 2.208 ± 0.278
2.678IleLys: 2.678 ± 0.488
3.148IleLeu: 3.148 ± 0.408
1.409IleMet: 1.409 ± 0.248
2.396IleAsn: 2.396 ± 0.377
2.819IlePro: 2.819 ± 0.359
1.503IleGln: 1.503 ± 0.253
3.054IleArg: 3.054 ± 0.368
3.336IleSer: 3.336 ± 0.459
4.886IleThr: 4.886 ± 0.514
3.195IleVal: 3.195 ± 0.368
1.174IleTrp: 1.174 ± 0.378
1.456IleTyr: 1.456 ± 0.286
0.0IleXaa: 0.0 ± 0.0
Lys
5.731LysAla: 5.731 ± 0.721
0.329LysCys: 0.329 ± 0.129
3.054LysAsp: 3.054 ± 0.373
3.101LysGlu: 3.101 ± 0.401
1.362LysPhe: 1.362 ± 0.286
3.242LysGly: 3.242 ± 0.451
0.799LysHis: 0.799 ± 0.181
1.926LysIle: 1.926 ± 0.404
2.443LysLys: 2.443 ± 0.345
4.275LysLeu: 4.275 ± 0.407
1.315LysMet: 1.315 ± 0.24
1.785LysAsn: 1.785 ± 0.252
2.255LysPro: 2.255 ± 0.42
1.926LysGln: 1.926 ± 0.316
2.866LysArg: 2.866 ± 0.372
2.725LysSer: 2.725 ± 0.349
2.443LysThr: 2.443 ± 0.396
3.101LysVal: 3.101 ± 0.296
0.799LysTrp: 0.799 ± 0.208
1.597LysTyr: 1.597 ± 0.301
0.0LysXaa: 0.0 ± 0.0
Leu
9.443LeuAla: 9.443 ± 0.901
0.705LeuCys: 0.705 ± 0.186
4.463LeuAsp: 4.463 ± 0.484
5.074LeuGlu: 5.074 ± 0.487
2.02LeuPhe: 2.02 ± 0.3
5.356LeuGly: 5.356 ± 0.487
1.644LeuHis: 1.644 ± 0.33
4.134LeuIle: 4.134 ± 0.548
3.054LeuLys: 3.054 ± 0.466
4.98LeuLeu: 4.98 ± 0.625
2.584LeuMet: 2.584 ± 0.312
2.255LeuAsn: 2.255 ± 0.36
3.007LeuPro: 3.007 ± 0.442
2.161LeuGln: 2.161 ± 0.351
4.463LeuArg: 4.463 ± 0.485
4.181LeuSer: 4.181 ± 0.33
5.731LeuThr: 5.731 ± 0.52
5.731LeuVal: 5.731 ± 0.454
0.94LeuTrp: 0.94 ± 0.179
1.691LeuTyr: 1.691 ± 0.266
0.0LeuXaa: 0.0 ± 0.0
Met
2.114MetAla: 2.114 ± 0.303
0.188MetCys: 0.188 ± 0.093
1.832MetAsp: 1.832 ± 0.383
1.832MetGlu: 1.832 ± 0.293
0.752MetPhe: 0.752 ± 0.207
2.067MetGly: 2.067 ± 0.343
0.423MetHis: 0.423 ± 0.142
1.456MetIle: 1.456 ± 0.24
1.034MetLys: 1.034 ± 0.218
1.409MetLeu: 1.409 ± 0.218
0.564MetMet: 0.564 ± 0.177
1.081MetAsn: 1.081 ± 0.196
1.456MetPro: 1.456 ± 0.264
0.799MetGln: 0.799 ± 0.156
1.738MetArg: 1.738 ± 0.244
2.067MetSer: 2.067 ± 0.33
2.161MetThr: 2.161 ± 0.281
1.973MetVal: 1.973 ± 0.282
0.47MetTrp: 0.47 ± 0.132
1.034MetTyr: 1.034 ± 0.266
0.0MetXaa: 0.0 ± 0.0
Asn
3.57AsnAla: 3.57 ± 0.401
0.376AsnCys: 0.376 ± 0.138
1.926AsnAsp: 1.926 ± 0.397
1.926AsnGlu: 1.926 ± 0.296
1.315AsnPhe: 1.315 ± 0.394
3.664AsnGly: 3.664 ± 0.573
1.128AsnHis: 1.128 ± 0.239
2.161AsnIle: 2.161 ± 0.481
1.644AsnLys: 1.644 ± 0.273
2.913AsnLeu: 2.913 ± 0.332
0.705AsnMet: 0.705 ± 0.147
1.362AsnAsn: 1.362 ± 0.375
1.973AsnPro: 1.973 ± 0.379
1.221AsnGln: 1.221 ± 0.325
1.832AsnArg: 1.832 ± 0.329
2.067AsnSer: 2.067 ± 0.615
2.02AsnThr: 2.02 ± 0.493
2.02AsnVal: 2.02 ± 0.312
0.893AsnTrp: 0.893 ± 0.205
0.658AsnTyr: 0.658 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
5.778ProAla: 5.778 ± 0.454
0.423ProCys: 0.423 ± 0.18
3.852ProAsp: 3.852 ± 0.566
3.946ProGlu: 3.946 ± 0.604
1.785ProPhe: 1.785 ± 0.267
4.181ProGly: 4.181 ± 0.423
1.55ProHis: 1.55 ± 0.351
2.208ProIle: 2.208 ± 0.291
2.161ProLys: 2.161 ± 0.362
2.819ProLeu: 2.819 ± 0.383
0.846ProMet: 0.846 ± 0.198
1.597ProAsn: 1.597 ± 0.264
2.631ProPro: 2.631 ± 0.4
1.785ProGln: 1.785 ± 0.318
2.114ProArg: 2.114 ± 0.357
3.101ProSer: 3.101 ± 0.365
3.805ProThr: 3.805 ± 0.465
3.758ProVal: 3.758 ± 0.38
0.987ProTrp: 0.987 ± 0.216
1.409ProTyr: 1.409 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
3.383GlnAla: 3.383 ± 0.378
0.329GlnCys: 0.329 ± 0.131
1.597GlnAsp: 1.597 ± 0.269
2.114GlnGlu: 2.114 ± 0.26
0.799GlnPhe: 0.799 ± 0.164
3.289GlnGly: 3.289 ± 0.514
1.268GlnHis: 1.268 ± 0.279
2.02GlnIle: 2.02 ± 0.332
1.503GlnLys: 1.503 ± 0.224
2.913GlnLeu: 2.913 ± 0.455
0.705GlnMet: 0.705 ± 0.192
0.799GlnAsn: 0.799 ± 0.176
1.691GlnPro: 1.691 ± 0.255
1.315GlnGln: 1.315 ± 0.367
2.302GlnArg: 2.302 ± 0.374
1.879GlnSer: 1.879 ± 0.291
1.268GlnThr: 1.268 ± 0.289
1.973GlnVal: 1.973 ± 0.327
1.174GlnTrp: 1.174 ± 0.221
1.456GlnTyr: 1.456 ± 0.275
0.0GlnXaa: 0.0 ± 0.0
Arg
5.074ArgAla: 5.074 ± 0.496
0.705ArgCys: 0.705 ± 0.216
3.101ArgAsp: 3.101 ± 0.309
4.181ArgGlu: 4.181 ± 0.605
1.973ArgPhe: 1.973 ± 0.286
4.228ArgGly: 4.228 ± 0.479
1.081ArgHis: 1.081 ± 0.252
2.96ArgIle: 2.96 ± 0.328
3.195ArgLys: 3.195 ± 0.44
4.04ArgLeu: 4.04 ± 0.482
1.456ArgMet: 1.456 ± 0.266
1.362ArgAsn: 1.362 ± 0.223
3.242ArgPro: 3.242 ± 0.448
2.678ArgGln: 2.678 ± 0.342
3.57ArgArg: 3.57 ± 0.499
3.007ArgSer: 3.007 ± 0.384
3.429ArgThr: 3.429 ± 0.431
4.369ArgVal: 4.369 ± 0.496
1.456ArgTrp: 1.456 ± 0.285
1.268ArgTyr: 1.268 ± 0.278
0.0ArgXaa: 0.0 ± 0.0
Ser
5.731SerAla: 5.731 ± 0.625
0.564SerCys: 0.564 ± 0.159
3.476SerAsp: 3.476 ± 0.285
3.711SerGlu: 3.711 ± 0.445
1.879SerPhe: 1.879 ± 0.229
5.684SerGly: 5.684 ± 0.616
0.846SerHis: 0.846 ± 0.242
3.054SerIle: 3.054 ± 0.354
2.537SerLys: 2.537 ± 0.371
3.899SerLeu: 3.899 ± 0.399
1.738SerMet: 1.738 ± 0.233
1.832SerAsn: 1.832 ± 0.357
3.242SerPro: 3.242 ± 0.33
1.55SerGln: 1.55 ± 0.312
3.007SerArg: 3.007 ± 0.384
3.242SerSer: 3.242 ± 0.377
4.557SerThr: 4.557 ± 0.423
3.993SerVal: 3.993 ± 0.456
1.409SerTrp: 1.409 ± 0.207
1.926SerTyr: 1.926 ± 0.341
0.0SerXaa: 0.0 ± 0.0
Thr
6.483ThrAla: 6.483 ± 1.077
0.423ThrCys: 0.423 ± 0.148
4.886ThrAsp: 4.886 ± 0.54
4.322ThrGlu: 4.322 ± 0.673
2.537ThrPhe: 2.537 ± 0.309
5.215ThrGly: 5.215 ± 0.533
1.456ThrHis: 1.456 ± 0.258
4.228ThrIle: 4.228 ± 0.525
2.913ThrLys: 2.913 ± 0.357
4.557ThrLeu: 4.557 ± 0.407
1.456ThrMet: 1.456 ± 0.277
2.255ThrAsn: 2.255 ± 0.464
4.134ThrPro: 4.134 ± 0.561
2.114ThrGln: 2.114 ± 0.26
3.758ThrArg: 3.758 ± 0.392
3.805ThrSer: 3.805 ± 0.631
5.074ThrThr: 5.074 ± 0.565
5.45ThrVal: 5.45 ± 0.603
1.034ThrTrp: 1.034 ± 0.227
1.268ThrTyr: 1.268 ± 0.253
0.0ThrXaa: 0.0 ± 0.0
Val
6.436ValAla: 6.436 ± 0.602
0.564ValCys: 0.564 ± 0.174
4.698ValAsp: 4.698 ± 0.413
5.262ValGlu: 5.262 ± 0.614
1.644ValPhe: 1.644 ± 0.248
4.322ValGly: 4.322 ± 0.528
1.034ValHis: 1.034 ± 0.241
4.228ValIle: 4.228 ± 0.402
2.678ValLys: 2.678 ± 0.355
4.604ValLeu: 4.604 ± 0.615
1.409ValMet: 1.409 ± 0.213
2.96ValAsn: 2.96 ± 0.437
3.242ValPro: 3.242 ± 0.34
1.973ValGln: 1.973 ± 0.249
4.228ValArg: 4.228 ± 0.455
3.429ValSer: 3.429 ± 0.457
6.154ValThr: 6.154 ± 0.603
5.074ValVal: 5.074 ± 0.475
1.034ValTrp: 1.034 ± 0.28
1.503ValTyr: 1.503 ± 0.297
0.0ValXaa: 0.0 ± 0.0
Trp
1.597TrpAla: 1.597 ± 0.274
0.282TrpCys: 0.282 ± 0.134
1.926TrpAsp: 1.926 ± 0.318
1.221TrpGlu: 1.221 ± 0.211
0.752TrpPhe: 0.752 ± 0.165
1.738TrpGly: 1.738 ± 0.228
0.423TrpHis: 0.423 ± 0.138
1.456TrpIle: 1.456 ± 0.314
0.799TrpLys: 0.799 ± 0.269
1.644TrpLeu: 1.644 ± 0.266
0.47TrpMet: 0.47 ± 0.122
0.799TrpAsn: 0.799 ± 0.363
1.128TrpPro: 1.128 ± 0.231
0.846TrpGln: 0.846 ± 0.203
1.034TrpArg: 1.034 ± 0.231
1.221TrpSer: 1.221 ± 0.225
1.268TrpThr: 1.268 ± 0.266
1.081TrpVal: 1.081 ± 0.23
0.329TrpTrp: 0.329 ± 0.142
0.705TrpTyr: 0.705 ± 0.182
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.067TyrAla: 2.067 ± 0.3
0.282TyrCys: 0.282 ± 0.126
2.819TyrAsp: 2.819 ± 0.393
1.879TyrGlu: 1.879 ± 0.402
1.174TyrPhe: 1.174 ± 0.208
1.879TyrGly: 1.879 ± 0.387
0.141TyrHis: 0.141 ± 0.087
1.456TyrIle: 1.456 ± 0.302
1.362TyrLys: 1.362 ± 0.294
2.114TyrLeu: 2.114 ± 0.387
0.611TyrMet: 0.611 ± 0.158
0.846TyrAsn: 0.846 ± 0.192
1.456TyrPro: 1.456 ± 0.256
0.799TyrGln: 0.799 ± 0.226
1.691TyrArg: 1.691 ± 0.321
1.597TyrSer: 1.597 ± 0.261
2.067TyrThr: 2.067 ± 0.387
1.55TyrVal: 1.55 ± 0.31
0.517TyrTrp: 0.517 ± 0.172
1.034TyrTyr: 1.034 ± 0.252
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 109 proteins (21287 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski