Mycobacterium phage Shandong1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9SH30|A0A1X9SH30_9CAUD Portal protein OS=Mycobacterium phage Shandong1 OX=1983447 PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 5.35APSTIEE8 pKa = 4.15VPCPACGEE16 pKa = 4.33PIILSIGFEE25 pKa = 4.31VVMPEE30 pKa = 4.19PDD32 pKa = 3.88ADD34 pKa = 3.91TAPVRR39 pKa = 11.84VTTPDD44 pKa = 3.36LADD47 pKa = 3.8RR48 pKa = 11.84AQAHH52 pKa = 6.24GEE54 pKa = 4.15VCPVLSGGGRR64 pKa = 3.27

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9SHM7|A0A1X9SHM7_9CAUD Uncharacterized protein OS=Mycobacterium phage Shandong1 OX=1983447 PE=4 SV=1
MM1 pKa = 7.42TNTMTMSEE9 pKa = 4.05ARR11 pKa = 11.84RR12 pKa = 11.84ITTALIAEE20 pKa = 4.72HH21 pKa = 6.81GLVGWTVKK29 pKa = 10.3FDD31 pKa = 3.58NARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AGQCSYY42 pKa = 11.34GPRR45 pKa = 11.84TISLSKK51 pKa = 10.33PLMAQRR57 pKa = 11.84SYY59 pKa = 11.69ADD61 pKa = 3.28TMNTITHH68 pKa = 6.64EE69 pKa = 4.06LAHH72 pKa = 6.52ALVGHH77 pKa = 5.46SHH79 pKa = 5.67GHH81 pKa = 5.8DD82 pKa = 3.43AVWSAKK88 pKa = 9.23HH89 pKa = 5.49RR90 pKa = 11.84QLGGDD95 pKa = 3.93GKK97 pKa = 11.01RR98 pKa = 11.84CFDD101 pKa = 4.64HH102 pKa = 8.28LDD104 pKa = 3.62EE105 pKa = 4.49SAPWIGTCGHH115 pKa = 6.78GKK117 pKa = 9.32QFARR121 pKa = 11.84YY122 pKa = 7.65RR123 pKa = 11.84QPKK126 pKa = 9.6RR127 pKa = 11.84LDD129 pKa = 2.91GWRR132 pKa = 11.84CRR134 pKa = 11.84CVRR137 pKa = 11.84GGSPITWQTRR147 pKa = 11.84AQRR150 pKa = 11.84ATEE153 pKa = 3.87ARR155 pKa = 11.84VVASVEE161 pKa = 3.95ARR163 pKa = 11.84KK164 pKa = 9.08AAAPAPVAAQASRR177 pKa = 11.84SITSAPVGKK186 pKa = 10.07GVQLGLFF193 pKa = 3.93

Molecular weight: 21.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

17995

37

1353

187.4

20.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.015 ± 0.497

1.184 ± 0.155

6.53 ± 0.237

5.852 ± 0.406

2.751 ± 0.186

8.647 ± 0.439

2.278 ± 0.189

3.762 ± 0.181

3.384 ± 0.175

8.447 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.173 ± 0.117

2.69 ± 0.209

5.602 ± 0.23

3.34 ± 0.159

7.208 ± 0.386

5.001 ± 0.262

5.752 ± 0.202

7.902 ± 0.24

2.167 ± 0.105

2.312 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski