Amino acid dipepetide frequency for Escherichia phage vB_EcoS_VAH1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.233AlaAla: 7.233 ± 0.979
0.7AlaCys: 0.7 ± 0.131
3.383AlaAsp: 3.383 ± 0.349
5.191AlaGlu: 5.191 ± 0.384
3.033AlaPhe: 3.033 ± 0.316
4.9AlaGly: 4.9 ± 0.458
1.575AlaHis: 1.575 ± 0.233
4.871AlaIle: 4.871 ± 0.397
6.3AlaLys: 6.3 ± 0.509
6.562AlaLeu: 6.562 ± 0.454
2.1AlaMet: 2.1 ± 0.245
3.733AlaAsn: 3.733 ± 0.383
2.246AlaPro: 2.246 ± 0.293
3.208AlaGln: 3.208 ± 0.32
3.179AlaArg: 3.179 ± 0.327
4.666AlaSer: 4.666 ± 0.662
3.529AlaThr: 3.529 ± 0.389
4.783AlaVal: 4.783 ± 0.367
0.787AlaTrp: 0.787 ± 0.169
2.275AlaTyr: 2.275 ± 0.266
0.0AlaXaa: 0.0 ± 0.0
Cys
0.758CysAla: 0.758 ± 0.154
0.292CysCys: 0.292 ± 0.114
0.496CysAsp: 0.496 ± 0.133
0.787CysGlu: 0.787 ± 0.135
0.612CysPhe: 0.612 ± 0.116
0.817CysGly: 0.817 ± 0.172
0.233CysHis: 0.233 ± 0.089
0.671CysIle: 0.671 ± 0.122
0.758CysLys: 0.758 ± 0.153
0.933CysLeu: 0.933 ± 0.187
0.35CysMet: 0.35 ± 0.113
0.7CysAsn: 0.7 ± 0.122
0.525CysPro: 0.525 ± 0.132
0.321CysGln: 0.321 ± 0.109
0.7CysArg: 0.7 ± 0.138
1.021CysSer: 1.021 ± 0.18
0.642CysThr: 0.642 ± 0.134
0.583CysVal: 0.583 ± 0.149
0.117CysTrp: 0.117 ± 0.06
0.467CysTyr: 0.467 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
3.996AspAla: 3.996 ± 0.365
0.787AspCys: 0.787 ± 0.14
2.887AspAsp: 2.887 ± 0.429
3.996AspGlu: 3.996 ± 0.339
2.537AspPhe: 2.537 ± 0.271
3.908AspGly: 3.908 ± 0.295
1.283AspHis: 1.283 ± 0.2
4.2AspIle: 4.2 ± 0.426
4.462AspLys: 4.462 ± 0.387
5.6AspLeu: 5.6 ± 0.502
1.925AspMet: 1.925 ± 0.206
2.654AspAsn: 2.654 ± 0.297
2.683AspPro: 2.683 ± 0.263
1.312AspGln: 1.312 ± 0.186
2.683AspArg: 2.683 ± 0.269
3.587AspSer: 3.587 ± 0.357
3.296AspThr: 3.296 ± 0.286
2.887AspVal: 2.887 ± 0.268
0.787AspTrp: 0.787 ± 0.153
2.566AspTyr: 2.566 ± 0.32
0.0AspXaa: 0.0 ± 0.0
Glu
5.6GluAla: 5.6 ± 0.399
0.875GluCys: 0.875 ± 0.176
3.471GluAsp: 3.471 ± 0.361
5.25GluGlu: 5.25 ± 0.515
2.625GluPhe: 2.625 ± 0.271
3.937GluGly: 3.937 ± 0.343
1.342GluHis: 1.342 ± 0.179
4.725GluIle: 4.725 ± 0.394
4.696GluLys: 4.696 ± 0.409
7.175GluLeu: 7.175 ± 0.453
2.333GluMet: 2.333 ± 0.304
2.741GluAsn: 2.741 ± 0.322
1.4GluPro: 1.4 ± 0.231
3.15GluGln: 3.15 ± 0.331
3.091GluArg: 3.091 ± 0.339
3.529GluSer: 3.529 ± 0.29
4.346GluThr: 4.346 ± 0.392
4.812GluVal: 4.812 ± 0.361
0.875GluTrp: 0.875 ± 0.186
3.062GluTyr: 3.062 ± 0.337
0.0GluXaa: 0.0 ± 0.0
Phe
2.479PheAla: 2.479 ± 0.264
0.321PheCys: 0.321 ± 0.1
2.829PheAsp: 2.829 ± 0.344
2.45PheGlu: 2.45 ± 0.257
1.254PhePhe: 1.254 ± 0.204
2.654PheGly: 2.654 ± 0.301
1.108PheHis: 1.108 ± 0.199
2.829PheIle: 2.829 ± 0.303
3.091PheLys: 3.091 ± 0.303
3.587PheLeu: 3.587 ± 0.291
0.729PheMet: 0.729 ± 0.134
2.596PheAsn: 2.596 ± 0.264
1.371PhePro: 1.371 ± 0.193
0.992PheGln: 0.992 ± 0.156
1.662PheArg: 1.662 ± 0.28
3.121PheSer: 3.121 ± 0.271
2.362PheThr: 2.362 ± 0.252
2.8PheVal: 2.8 ± 0.285
0.583PheTrp: 0.583 ± 0.142
1.604PheTyr: 1.604 ± 0.214
0.0PheXaa: 0.0 ± 0.0
Gly
4.054GlyAla: 4.054 ± 0.461
0.992GlyCys: 0.992 ± 0.185
3.529GlyAsp: 3.529 ± 0.36
4.521GlyGlu: 4.521 ± 0.327
3.383GlyPhe: 3.383 ± 0.297
3.441GlyGly: 3.441 ± 0.421
1.05GlyHis: 1.05 ± 0.175
4.754GlyIle: 4.754 ± 0.419
5.337GlyLys: 5.337 ± 0.42
4.725GlyLeu: 4.725 ± 0.393
2.042GlyMet: 2.042 ± 0.244
3.616GlyAsn: 3.616 ± 0.427
0.962GlyPro: 0.962 ± 0.178
2.071GlyGln: 2.071 ± 0.266
2.975GlyArg: 2.975 ± 0.285
4.025GlySer: 4.025 ± 0.345
3.529GlyThr: 3.529 ± 0.339
4.608GlyVal: 4.608 ± 0.364
0.846GlyTrp: 0.846 ± 0.146
3.296GlyTyr: 3.296 ± 0.341
0.0GlyXaa: 0.0 ± 0.0
His
0.904HisAla: 0.904 ± 0.185
0.408HisCys: 0.408 ± 0.116
1.283HisAsp: 1.283 ± 0.201
0.992HisGlu: 0.992 ± 0.187
0.496HisPhe: 0.496 ± 0.158
1.342HisGly: 1.342 ± 0.204
0.554HisHis: 0.554 ± 0.147
1.342HisIle: 1.342 ± 0.227
1.429HisLys: 1.429 ± 0.2
2.071HisLeu: 2.071 ± 0.256
0.292HisMet: 0.292 ± 0.118
1.167HisAsn: 1.167 ± 0.221
0.904HisPro: 0.904 ± 0.18
0.583HisGln: 0.583 ± 0.128
1.371HisArg: 1.371 ± 0.204
1.108HisSer: 1.108 ± 0.216
0.904HisThr: 0.904 ± 0.167
0.904HisVal: 0.904 ± 0.171
0.117HisTrp: 0.117 ± 0.075
0.933HisTyr: 0.933 ± 0.162
0.0HisXaa: 0.0 ± 0.0
Ile
5.308IleAla: 5.308 ± 0.4
0.904IleCys: 0.904 ± 0.208
4.783IleAsp: 4.783 ± 0.401
4.637IleGlu: 4.637 ± 0.398
2.275IlePhe: 2.275 ± 0.232
4.229IleGly: 4.229 ± 0.303
1.312IleHis: 1.312 ± 0.184
4.287IleIle: 4.287 ± 0.368
4.346IleLys: 4.346 ± 0.433
5.483IleLeu: 5.483 ± 0.377
1.75IleMet: 1.75 ± 0.296
4.141IleAsn: 4.141 ± 0.381
2.8IlePro: 2.8 ± 0.331
2.158IleGln: 2.158 ± 0.254
2.654IleArg: 2.654 ± 0.27
4.375IleSer: 4.375 ± 0.354
4.608IleThr: 4.608 ± 0.383
3.529IleVal: 3.529 ± 0.298
0.496IleTrp: 0.496 ± 0.12
2.566IleTyr: 2.566 ± 0.292
0.0IleXaa: 0.0 ± 0.0
Lys
6.387LysAla: 6.387 ± 0.467
0.642LysCys: 0.642 ± 0.138
4.666LysAsp: 4.666 ± 0.401
5.191LysGlu: 5.191 ± 0.45
3.558LysPhe: 3.558 ± 0.264
3.733LysGly: 3.733 ± 0.353
1.546LysHis: 1.546 ± 0.216
4.258LysIle: 4.258 ± 0.398
4.987LysLys: 4.987 ± 0.414
6.766LysLeu: 6.766 ± 0.517
2.246LysMet: 2.246 ± 0.314
3.85LysAsn: 3.85 ± 0.369
2.333LysPro: 2.333 ± 0.266
3.208LysGln: 3.208 ± 0.302
3.762LysArg: 3.762 ± 0.296
4.579LysSer: 4.579 ± 0.47
4.141LysThr: 4.141 ± 0.398
4.696LysVal: 4.696 ± 0.365
0.729LysTrp: 0.729 ± 0.161
3.121LysTyr: 3.121 ± 0.258
0.0LysXaa: 0.0 ± 0.0
Leu
7.262LeuAla: 7.262 ± 0.457
0.817LeuCys: 0.817 ± 0.15
6.095LeuAsp: 6.095 ± 0.449
7.175LeuGlu: 7.175 ± 0.408
2.946LeuPhe: 2.946 ± 0.317
5.045LeuGly: 5.045 ± 0.425
1.925LeuHis: 1.925 ± 0.245
5.337LeuIle: 5.337 ± 0.467
6.125LeuLys: 6.125 ± 0.428
6.737LeuLeu: 6.737 ± 0.512
2.129LeuMet: 2.129 ± 0.318
5.22LeuAsn: 5.22 ± 0.451
3.529LeuPro: 3.529 ± 0.298
3.383LeuGln: 3.383 ± 0.343
4.229LeuArg: 4.229 ± 0.319
4.929LeuSer: 4.929 ± 0.354
5.191LeuThr: 5.191 ± 0.369
5.308LeuVal: 5.308 ± 0.429
0.817LeuTrp: 0.817 ± 0.158
2.8LeuTyr: 2.8 ± 0.313
0.0LeuXaa: 0.0 ± 0.0
Met
1.867MetAla: 1.867 ± 0.241
0.292MetCys: 0.292 ± 0.104
1.079MetAsp: 1.079 ± 0.201
2.158MetGlu: 2.158 ± 0.286
0.846MetPhe: 0.846 ± 0.178
1.4MetGly: 1.4 ± 0.171
0.525MetHis: 0.525 ± 0.124
1.983MetIle: 1.983 ± 0.279
2.625MetLys: 2.625 ± 0.301
2.596MetLeu: 2.596 ± 0.353
0.583MetMet: 0.583 ± 0.131
0.992MetAsn: 0.992 ± 0.176
0.875MetPro: 0.875 ± 0.161
1.108MetGln: 1.108 ± 0.225
1.079MetArg: 1.079 ± 0.163
2.362MetSer: 2.362 ± 0.3
1.75MetThr: 1.75 ± 0.195
1.429MetVal: 1.429 ± 0.198
0.262MetTrp: 0.262 ± 0.087
0.962MetTyr: 0.962 ± 0.169
0.0MetXaa: 0.0 ± 0.0
Asn
3.558AsnAla: 3.558 ± 0.457
0.7AsnCys: 0.7 ± 0.15
2.741AsnAsp: 2.741 ± 0.264
2.946AsnGlu: 2.946 ± 0.316
2.246AsnPhe: 2.246 ± 0.296
4.229AsnGly: 4.229 ± 0.469
0.933AsnHis: 0.933 ± 0.201
3.383AsnIle: 3.383 ± 0.304
4.375AsnLys: 4.375 ± 0.477
4.637AsnLeu: 4.637 ± 0.383
1.342AsnMet: 1.342 ± 0.23
3.5AsnAsn: 3.5 ± 0.351
2.741AsnPro: 2.741 ± 0.272
1.721AsnGln: 1.721 ± 0.29
2.829AsnArg: 2.829 ± 0.296
3.762AsnSer: 3.762 ± 0.331
3.062AsnThr: 3.062 ± 0.279
3.5AsnVal: 3.5 ± 0.293
0.554AsnTrp: 0.554 ± 0.117
1.75AsnTyr: 1.75 ± 0.222
0.0AsnXaa: 0.0 ± 0.0
Pro
2.741ProAla: 2.741 ± 0.283
0.379ProCys: 0.379 ± 0.104
2.217ProAsp: 2.217 ± 0.267
3.354ProGlu: 3.354 ± 0.291
1.487ProPhe: 1.487 ± 0.225
2.129ProGly: 2.129 ± 0.275
0.642ProHis: 0.642 ± 0.135
2.158ProIle: 2.158 ± 0.313
2.217ProLys: 2.217 ± 0.22
2.333ProLeu: 2.333 ± 0.264
0.583ProMet: 0.583 ± 0.123
2.042ProAsn: 2.042 ± 0.272
1.108ProPro: 1.108 ± 0.214
1.05ProGln: 1.05 ± 0.18
1.429ProArg: 1.429 ± 0.188
2.158ProSer: 2.158 ± 0.277
1.779ProThr: 1.779 ± 0.221
2.392ProVal: 2.392 ± 0.248
0.437ProTrp: 0.437 ± 0.121
1.692ProTyr: 1.692 ± 0.195
0.0ProXaa: 0.0 ± 0.0
Gln
2.946GlnAla: 2.946 ± 0.438
0.437GlnCys: 0.437 ± 0.114
1.867GlnAsp: 1.867 ± 0.23
2.771GlnGlu: 2.771 ± 0.326
1.517GlnPhe: 1.517 ± 0.198
1.75GlnGly: 1.75 ± 0.19
0.467GlnHis: 0.467 ± 0.139
2.566GlnIle: 2.566 ± 0.322
2.392GlnLys: 2.392 ± 0.293
4.054GlnLeu: 4.054 ± 0.396
1.05GlnMet: 1.05 ± 0.22
1.954GlnAsn: 1.954 ± 0.221
0.612GlnPro: 0.612 ± 0.155
2.479GlnGln: 2.479 ± 0.485
1.779GlnArg: 1.779 ± 0.23
1.954GlnSer: 1.954 ± 0.278
2.158GlnThr: 2.158 ± 0.315
2.508GlnVal: 2.508 ± 0.29
0.467GlnTrp: 0.467 ± 0.143
1.487GlnTyr: 1.487 ± 0.234
0.0GlnXaa: 0.0 ± 0.0
Arg
2.975ArgAla: 2.975 ± 0.334
0.467ArgCys: 0.467 ± 0.102
2.712ArgAsp: 2.712 ± 0.263
3.121ArgGlu: 3.121 ± 0.28
1.662ArgPhe: 1.662 ± 0.186
3.441ArgGly: 3.441 ± 0.265
0.642ArgHis: 0.642 ± 0.15
3.208ArgIle: 3.208 ± 0.26
3.675ArgLys: 3.675 ± 0.402
4.229ArgLeu: 4.229 ± 0.369
1.371ArgMet: 1.371 ± 0.235
2.566ArgAsn: 2.566 ± 0.266
1.487ArgPro: 1.487 ± 0.195
1.662ArgGln: 1.662 ± 0.195
2.362ArgArg: 2.362 ± 0.295
2.508ArgSer: 2.508 ± 0.277
2.625ArgThr: 2.625 ± 0.234
3.033ArgVal: 3.033 ± 0.307
0.7ArgTrp: 0.7 ± 0.148
1.896ArgTyr: 1.896 ± 0.258
0.0ArgXaa: 0.0 ± 0.0
Ser
4.462SerAla: 4.462 ± 0.553
0.846SerCys: 0.846 ± 0.19
3.033SerAsp: 3.033 ± 0.324
3.762SerGlu: 3.762 ± 0.485
2.946SerPhe: 2.946 ± 0.284
5.016SerGly: 5.016 ± 0.475
0.817SerHis: 0.817 ± 0.135
4.2SerIle: 4.2 ± 0.38
4.725SerLys: 4.725 ± 0.37
6.183SerLeu: 6.183 ± 0.484
1.575SerMet: 1.575 ± 0.2
3.412SerAsn: 3.412 ± 0.332
1.954SerPro: 1.954 ± 0.251
2.071SerGln: 2.071 ± 0.316
3.121SerArg: 3.121 ± 0.351
4.579SerSer: 4.579 ± 0.434
3.471SerThr: 3.471 ± 0.281
3.85SerVal: 3.85 ± 0.346
1.196SerTrp: 1.196 ± 0.189
2.508SerTyr: 2.508 ± 0.27
0.0SerXaa: 0.0 ± 0.0
Thr
3.996ThrAla: 3.996 ± 0.484
0.467ThrCys: 0.467 ± 0.101
2.887ThrAsp: 2.887 ± 0.312
3.383ThrGlu: 3.383 ± 0.265
2.333ThrPhe: 2.333 ± 0.248
4.958ThrGly: 4.958 ± 0.43
0.962ThrHis: 0.962 ± 0.173
4.025ThrIle: 4.025 ± 0.366
3.704ThrLys: 3.704 ± 0.371
4.433ThrLeu: 4.433 ± 0.36
1.283ThrMet: 1.283 ± 0.199
3.208ThrAsn: 3.208 ± 0.33
2.421ThrPro: 2.421 ± 0.216
2.421ThrGln: 2.421 ± 0.241
2.362ThrArg: 2.362 ± 0.231
3.675ThrSer: 3.675 ± 0.344
3.587ThrThr: 3.587 ± 0.309
4.112ThrVal: 4.112 ± 0.379
0.787ThrTrp: 0.787 ± 0.148
2.508ThrTyr: 2.508 ± 0.308
0.0ThrXaa: 0.0 ± 0.0
Val
4.725ValAla: 4.725 ± 0.372
0.612ValCys: 0.612 ± 0.142
4.346ValAsp: 4.346 ± 0.412
4.083ValGlu: 4.083 ± 0.358
2.537ValPhe: 2.537 ± 0.286
3.704ValGly: 3.704 ± 0.372
1.021ValHis: 1.021 ± 0.193
4.404ValIle: 4.404 ± 0.349
4.9ValLys: 4.9 ± 0.358
4.841ValLeu: 4.841 ± 0.34
1.633ValMet: 1.633 ± 0.209
3.004ValAsn: 3.004 ± 0.342
2.741ValPro: 2.741 ± 0.335
2.246ValGln: 2.246 ± 0.316
2.479ValArg: 2.479 ± 0.242
4.637ValSer: 4.637 ± 0.426
3.762ValThr: 3.762 ± 0.364
4.579ValVal: 4.579 ± 0.433
0.612ValTrp: 0.612 ± 0.142
2.508ValTyr: 2.508 ± 0.269
0.0ValXaa: 0.0 ± 0.0
Trp
0.612TrpAla: 0.612 ± 0.135
0.233TrpCys: 0.233 ± 0.082
0.758TrpAsp: 0.758 ± 0.129
1.137TrpGlu: 1.137 ± 0.2
0.554TrpPhe: 0.554 ± 0.137
0.612TrpGly: 0.612 ± 0.13
0.117TrpHis: 0.117 ± 0.064
0.583TrpIle: 0.583 ± 0.154
1.05TrpLys: 1.05 ± 0.161
1.167TrpLeu: 1.167 ± 0.205
0.408TrpMet: 0.408 ± 0.115
0.729TrpAsn: 0.729 ± 0.177
0.204TrpPro: 0.204 ± 0.079
0.554TrpGln: 0.554 ± 0.134
0.583TrpArg: 0.583 ± 0.134
0.729TrpSer: 0.729 ± 0.162
0.525TrpThr: 0.525 ± 0.131
0.817TrpVal: 0.817 ± 0.167
0.175TrpTrp: 0.175 ± 0.084
0.35TrpTyr: 0.35 ± 0.111
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.392TyrAla: 2.392 ± 0.307
0.612TyrCys: 0.612 ± 0.144
2.975TyrAsp: 2.975 ± 0.307
2.158TyrGlu: 2.158 ± 0.253
1.575TyrPhe: 1.575 ± 0.253
2.508TyrGly: 2.508 ± 0.236
1.05TyrHis: 1.05 ± 0.18
2.858TyrIle: 2.858 ± 0.318
3.208TyrLys: 3.208 ± 0.326
2.887TyrLeu: 2.887 ± 0.302
1.021TyrMet: 1.021 ± 0.174
2.683TyrAsn: 2.683 ± 0.245
1.429TyrPro: 1.429 ± 0.208
1.517TyrGln: 1.517 ± 0.201
2.012TyrArg: 2.012 ± 0.229
2.421TyrSer: 2.421 ± 0.312
2.246TyrThr: 2.246 ± 0.267
2.246TyrVal: 2.246 ± 0.269
0.554TyrTrp: 0.554 ± 0.132
1.546TyrTyr: 1.546 ± 0.237
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 195 proteins (34289 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski