Porcine associated porprismacovirus 4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076VJK7|A0A076VJK7_9VIRU Rep protein OS=Porcine associated porprismacovirus 4 OX=2170120 PE=4 SV=1
MM1 pKa = 8.17VMVRR5 pKa = 11.84VSEE8 pKa = 4.34TYY10 pKa = 10.68DD11 pKa = 3.26LSTKK15 pKa = 8.72VGKK18 pKa = 9.42MGIVGIHH25 pKa = 4.95TPKK28 pKa = 10.68GNLITRR34 pKa = 11.84HH35 pKa = 5.39YY36 pKa = 10.55GGLIQNFKK44 pKa = 8.66YY45 pKa = 10.82AKK47 pKa = 9.94FVSCDD52 pKa = 3.15VALACASMLPADD64 pKa = 4.61PLQIGQEE71 pKa = 3.93AGAIAPQDD79 pKa = 3.54MFNPILYY86 pKa = 9.41RR87 pKa = 11.84AVSNEE92 pKa = 3.38SMNNFLAFLQSGAILNGAVDD112 pKa = 4.06KK113 pKa = 11.65NSVIDD118 pKa = 4.16LNNTSFTFNEE128 pKa = 4.12AEE130 pKa = 3.8VDD132 pKa = 3.77QFQLYY137 pKa = 10.4YY138 pKa = 11.11GLLSNSDD145 pKa = 3.32GWKK148 pKa = 10.17KK149 pKa = 11.0AMPQAGLEE157 pKa = 4.04MRR159 pKa = 11.84GLYY162 pKa = 9.98PLVFSVNVNTAMPSANGIGKK182 pKa = 8.88QFDD185 pKa = 3.82SEE187 pKa = 4.49FTGLSNTEE195 pKa = 3.63ASPAKK200 pKa = 10.35VGTVQWMRR208 pKa = 11.84GGAVRR213 pKa = 11.84MPRR216 pKa = 11.84FPTIRR221 pKa = 11.84FGTNEE226 pKa = 3.87QYY228 pKa = 11.73YY229 pKa = 10.67LIGDD233 pKa = 3.79VAGKK237 pKa = 10.4SDD239 pKa = 4.44LSDD242 pKa = 3.01MSMNAYY248 pKa = 9.86VAMIVLPPAKK258 pKa = 10.05LNQPYY263 pKa = 10.08YY264 pKa = 9.57RR265 pKa = 11.84LKK267 pKa = 9.62VTWTVEE273 pKa = 3.82FSEE276 pKa = 4.69IRR278 pKa = 11.84SQADD282 pKa = 2.9ILNWHH287 pKa = 6.44SLAAIGDD294 pKa = 4.01VSCGTDD300 pKa = 3.12YY301 pKa = 11.37AAQSKK306 pKa = 10.56AMARR310 pKa = 11.84TEE312 pKa = 4.3GMVDD316 pKa = 3.17TNGADD321 pKa = 3.38VTKK324 pKa = 11.01VMDD327 pKa = 5.29GII329 pKa = 4.31

Molecular weight: 35.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076VJK7|A0A076VJK7_9VIRU Rep protein OS=Porcine associated porprismacovirus 4 OX=2170120 PE=4 SV=1
MM1 pKa = 6.19QTYY4 pKa = 10.75VMTIPRR10 pKa = 11.84TVSKK14 pKa = 10.35RR15 pKa = 11.84ALRR18 pKa = 11.84IMIEE22 pKa = 4.22KK23 pKa = 9.91NDD25 pKa = 3.67CKK27 pKa = 10.62KK28 pKa = 9.84WVIGKK33 pKa = 9.52EE34 pKa = 3.99EE35 pKa = 3.99GKK37 pKa = 10.26NGYY40 pKa = 8.24KK41 pKa = 9.71HH42 pKa = 4.18WQIRR46 pKa = 11.84IEE48 pKa = 4.08TSNDD52 pKa = 3.27DD53 pKa = 3.58FFEE56 pKa = 3.99WMQDD60 pKa = 3.7HH61 pKa = 7.07IPTAHH66 pKa = 6.05VEE68 pKa = 3.97KK69 pKa = 10.9SEE71 pKa = 4.2NGVDD75 pKa = 3.03ACRR78 pKa = 11.84YY79 pKa = 5.01EE80 pKa = 4.44TKK82 pKa = 10.22EE83 pKa = 3.92GQYY86 pKa = 11.43VMYY89 pKa = 10.2SDD91 pKa = 5.14RR92 pKa = 11.84VQNLMQRR99 pKa = 11.84YY100 pKa = 8.21GAFRR104 pKa = 11.84PNQKK108 pKa = 9.94RR109 pKa = 11.84AMQALQATNDD119 pKa = 3.61RR120 pKa = 11.84QVVVWYY126 pKa = 10.21DD127 pKa = 3.2EE128 pKa = 4.14TGNVGKK134 pKa = 10.24SWFTGALWEE143 pKa = 4.56RR144 pKa = 11.84GLAYY148 pKa = 7.93VTPPTIDD155 pKa = 3.16TVKK158 pKa = 11.09GLIQWVASCYY168 pKa = 9.78IDD170 pKa = 4.44GGWRR174 pKa = 11.84PYY176 pKa = 11.0VIIDD180 pKa = 4.19VPRR183 pKa = 11.84SWKK186 pKa = 9.71WSEE189 pKa = 3.8QLYY192 pKa = 10.74SAIEE196 pKa = 4.22SIKK199 pKa = 11.0DD200 pKa = 3.09GLIYY204 pKa = 9.1DD205 pKa = 4.01TRR207 pKa = 11.84YY208 pKa = 8.56HH209 pKa = 6.86SRR211 pKa = 11.84MINIRR216 pKa = 11.84GVKK219 pKa = 9.03VLVMTNTMPKK229 pKa = 9.86LDD231 pKa = 4.6KK232 pKa = 10.62LSKK235 pKa = 10.6DD236 pKa = 3.0RR237 pKa = 11.84WCICTFF243 pKa = 3.64

Molecular weight: 28.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

572

243

329

286.0

32.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.517 ± 1.974

1.399 ± 0.435

5.769 ± 0.266

4.371 ± 0.646

3.322 ± 0.835

7.517 ± 0.888

1.224 ± 0.279

6.119 ± 0.851

5.769 ± 1.081

6.294 ± 1.166

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.72 ± 0.399

5.42 ± 0.861

3.846 ± 0.366

4.545 ± 0.259

4.895 ± 1.387

6.294 ± 0.895

5.944 ± 0.423

7.867 ± 0.032

2.622 ± 1.257

4.545 ± 0.531
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski