Methylobacterium sp. Leaf99

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium; unclassified Methylobacterium

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4XWP5|A0A0Q4XWP5_9RHIZ ATP-dependent Clp protease proteolytic subunit OS=Methylobacterium sp. Leaf99 OX=1736251 GN=ASF28_08965 PE=3 SV=1
MM1 pKa = 7.57LEE3 pKa = 4.76SIPAGWPCALLPAEE17 pKa = 4.84PPSVADD23 pKa = 3.98RR24 pKa = 11.84QSNPSADD31 pKa = 3.93EE32 pKa = 3.82ILPQVLALTPRR43 pKa = 11.84GAAWGADD50 pKa = 3.31EE51 pKa = 5.96AGDD54 pKa = 3.9GKK56 pKa = 10.89GASPVQRR63 pKa = 11.84SFWRR67 pKa = 11.84GIAAWVADD75 pKa = 4.12LNIRR79 pKa = 11.84DD80 pKa = 3.81WTIATQTFPSAATVSLPDD98 pKa = 3.35WEE100 pKa = 5.85AEE102 pKa = 4.03LGLPDD107 pKa = 3.83PCMPAGRR114 pKa = 11.84TAEE117 pKa = 3.9QRR119 pKa = 11.84VSRR122 pKa = 11.84VRR124 pKa = 11.84ARR126 pKa = 11.84FGAQGGASPAYY137 pKa = 8.3FVCVAASLGFTMRR150 pKa = 11.84VEE152 pKa = 4.23EE153 pKa = 4.28PTQFLCDD160 pKa = 3.47VSEE163 pKa = 4.53CVAFDD168 pKa = 3.59TLDD171 pKa = 3.29TYY173 pKa = 7.52FTCDD177 pKa = 3.09EE178 pKa = 4.68AEE180 pKa = 4.4CGEE183 pKa = 4.88DD184 pKa = 3.52GDD186 pKa = 4.92PIEE189 pKa = 5.93GYY191 pKa = 10.49AFPSAADD198 pKa = 3.97PGDD201 pKa = 3.88EE202 pKa = 4.33VAGGALNEE210 pKa = 4.52TYY212 pKa = 10.13FACDD216 pKa = 3.03EE217 pKa = 4.66GEE219 pKa = 4.26CGEE222 pKa = 4.76DD223 pKa = 3.48GDD225 pKa = 4.9RR226 pKa = 11.84IEE228 pKa = 6.07GFDD231 pKa = 4.17PNDD234 pKa = 3.95PGAVAWRR241 pKa = 11.84FWVADD246 pKa = 3.7VVTAPATYY254 pKa = 9.91FRR256 pKa = 11.84CDD258 pKa = 3.22EE259 pKa = 4.49AEE261 pKa = 4.42CGAEE265 pKa = 3.86GDD267 pKa = 4.2RR268 pKa = 11.84IEE270 pKa = 5.66GYY272 pKa = 8.66TPAPDD277 pKa = 4.17LEE279 pKa = 4.6CVLARR284 pKa = 11.84LSPPHH289 pKa = 5.31TTLVFRR295 pKa = 11.84YY296 pKa = 9.24PDD298 pKa = 3.03

Molecular weight: 31.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4XPX2|A0A0Q4XPX2_9RHIZ General stress protein CsbD OS=Methylobacterium sp. Leaf99 OX=1736251 GN=ASF28_02825 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.07VIAARR34 pKa = 11.84RR35 pKa = 11.84SHH37 pKa = 5.5GRR39 pKa = 11.84KK40 pKa = 9.35KK41 pKa = 10.69LSAA44 pKa = 3.99

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4109

0

4109

1270044

29

3736

309.1

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.375 ± 0.056

0.774 ± 0.013

5.741 ± 0.028

5.255 ± 0.04

3.388 ± 0.026

9.236 ± 0.046

1.968 ± 0.021

4.405 ± 0.026

2.542 ± 0.032

10.393 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.04 ± 0.018

2.1 ± 0.024

5.796 ± 0.032

2.636 ± 0.025

7.983 ± 0.045

4.74 ± 0.027

5.671 ± 0.04

7.761 ± 0.031

1.204 ± 0.017

1.991 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski