Micromonospora sp. 4G51

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; unclassified Micromonospora

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6887 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A317DN82|A0A317DN82_9ACTN Uncharacterized protein (Fragment) OS=Micromonospora sp. 4G51 OX=2202420 GN=DKT69_10200 PE=4 SV=1
MM1 pKa = 6.97TALAFTVSAPAQANDD16 pKa = 3.58HH17 pKa = 6.55EE18 pKa = 4.66NLPGEE23 pKa = 4.2PTITLPINDD32 pKa = 3.78SAAGGTYY39 pKa = 10.09NQDD42 pKa = 3.11FTRR45 pKa = 11.84VYY47 pKa = 11.41ANTTPDD53 pKa = 3.31GTPVYY58 pKa = 10.08IYY60 pKa = 10.52VPKK63 pKa = 9.73GTPLDD68 pKa = 3.67GTAPAGVASLDD79 pKa = 3.44PQFDD83 pKa = 4.12HH84 pKa = 7.02NPADD88 pKa = 3.74GFTPCATATAAQFTLTQAEE107 pKa = 4.2INYY110 pKa = 10.07VGDD113 pKa = 3.67KK114 pKa = 10.85LADD117 pKa = 3.45QIVAVDD123 pKa = 3.83EE124 pKa = 4.23EE125 pKa = 5.47HH126 pKa = 6.64YY127 pKa = 11.36GPMDD131 pKa = 3.9AADD134 pKa = 3.98PAEE137 pKa = 4.4PASDD141 pKa = 3.6SLVMVLYY148 pKa = 10.64NIQDD152 pKa = 3.5DD153 pKa = 4.51AYY155 pKa = 9.53YY156 pKa = 10.96DD157 pKa = 3.96CAQTSYY163 pKa = 9.62TAGYY167 pKa = 7.63FAPDD171 pKa = 4.9FIDD174 pKa = 3.53SVGMNVIVVDD184 pKa = 3.98ALDD187 pKa = 3.24WANRR191 pKa = 11.84VGPNDD196 pKa = 4.06SPWRR200 pKa = 11.84DD201 pKa = 3.26ANPANDD207 pKa = 3.77RR208 pKa = 11.84PEE210 pKa = 4.76LYY212 pKa = 9.95EE213 pKa = 3.8GTVAHH218 pKa = 6.86EE219 pKa = 4.26LQHH222 pKa = 6.7LLHH225 pKa = 6.77NYY227 pKa = 9.69SDD229 pKa = 4.0PGEE232 pKa = 4.2LSWVDD237 pKa = 3.2EE238 pKa = 4.25GLADD242 pKa = 4.89FAIFLNGLDD251 pKa = 3.78AGGSHH256 pKa = 7.78LSNQQVFYY264 pKa = 11.03EE265 pKa = 4.46EE266 pKa = 4.2TSLTRR271 pKa = 11.84WGGTLANYY279 pKa = 7.71GASFTYY285 pKa = 9.52FQYY288 pKa = 11.02LWEE291 pKa = 4.13QAGGNGDD298 pKa = 3.71GTYY301 pKa = 10.08TPDD304 pKa = 3.62KK305 pKa = 10.99QYY307 pKa = 11.45DD308 pKa = 3.93DD309 pKa = 3.9KK310 pKa = 11.99AGDD313 pKa = 4.09LLIKK317 pKa = 9.82TIFEE321 pKa = 4.07EE322 pKa = 4.52QADD325 pKa = 3.96GMTGVQNAIDD335 pKa = 4.61DD336 pKa = 4.32FNAATGSHH344 pKa = 6.09LRR346 pKa = 11.84SAADD350 pKa = 4.09LFRR353 pKa = 11.84DD354 pKa = 3.46WSVAVYY360 pKa = 10.72LDD362 pKa = 4.64DD363 pKa = 5.03EE364 pKa = 4.56NSPTYY369 pKa = 10.08DD370 pKa = 2.4IKK372 pKa = 11.3AFNFGDD378 pKa = 3.91PADD381 pKa = 4.16TAWTIDD387 pKa = 3.2IADD390 pKa = 3.78DD391 pKa = 4.0VFWSGRR397 pKa = 11.84GSYY400 pKa = 9.81QGATPEE406 pKa = 4.3AKK408 pKa = 9.29WARR411 pKa = 11.84LKK413 pKa = 10.72NRR415 pKa = 11.84PDD417 pKa = 3.11TTAVPFGMSVEE428 pKa = 4.27RR429 pKa = 11.84FRR431 pKa = 11.84NPGPTVAVAFDD442 pKa = 4.51GDD444 pKa = 4.4DD445 pKa = 3.37QTVIAPHH452 pKa = 6.61TGTTHH457 pKa = 6.59WYY459 pKa = 10.12AGYY462 pKa = 10.32EE463 pKa = 4.16SQSDD467 pKa = 3.84NILNVDD473 pKa = 3.34TAGPVSSLDD482 pKa = 3.27FWSWNFIEE490 pKa = 5.16EE491 pKa = 4.13GWDD494 pKa = 3.28YY495 pKa = 11.62GFAEE499 pKa = 4.64ALVGGDD505 pKa = 2.98WVTVPLRR512 pKa = 11.84NDD514 pKa = 3.02AGQVVTTNDD523 pKa = 3.47NPHH526 pKa = 6.32GNNTEE531 pKa = 4.3GNGLTGTSGGEE542 pKa = 4.02YY543 pKa = 10.35FVDD546 pKa = 3.79DD547 pKa = 4.16PVYY550 pKa = 11.1VHH552 pKa = 6.54LTANLPAGATDD563 pKa = 3.03VRR565 pKa = 11.84FRR567 pKa = 11.84YY568 pKa = 8.58STDD571 pKa = 2.83AAYY574 pKa = 10.92LDD576 pKa = 4.21TGWFVDD582 pKa = 3.9DD583 pKa = 4.01VRR585 pKa = 11.84VNGADD590 pKa = 3.3ATLSSPAGDD599 pKa = 3.13WFSTTGTQDD608 pKa = 3.32NNWTVQVVSHH618 pKa = 7.15CDD620 pKa = 3.41LTPGTTSAGEE630 pKa = 4.06ITDD633 pKa = 4.08GAGNWVYY640 pKa = 11.01RR641 pKa = 11.84FTGDD645 pKa = 2.7QFTRR649 pKa = 11.84SGLQTKK655 pKa = 10.03CAGGNQDD662 pKa = 4.97DD663 pKa = 4.27FAVLISNLPTGDD675 pKa = 3.92LTFLDD680 pKa = 3.43ADD682 pKa = 3.68YY683 pKa = 10.41TFRR686 pKa = 11.84VTNTGNKK693 pKa = 9.04KK694 pKa = 10.25

Molecular weight: 75.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A317DJ32|A0A317DJ32_9ACTN Aquaporin OS=Micromonospora sp. 4G51 OX=2202420 GN=DKT69_14625 PE=3 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.49KK7 pKa = 9.45RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6887

0

6887

2147859

21

3225

311.9

33.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.97 ± 0.044

0.761 ± 0.009

6.058 ± 0.023

5.056 ± 0.028

2.683 ± 0.017

9.294 ± 0.032

2.177 ± 0.017

3.2 ± 0.02

1.804 ± 0.022

10.528 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.608 ± 0.013

1.824 ± 0.021

6.269 ± 0.027

2.812 ± 0.018

8.564 ± 0.041

4.643 ± 0.023

6.059 ± 0.03

8.941 ± 0.03

1.651 ± 0.013

2.098 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski